IR@PKUHSC  > 北京大学口腔医学院  > 口腔病理科
学科主题口腔医学
Generation of homologous cell pairs using the oral lymphatic system
Pu, Yin-Fei1; Wang, Lin1; Wu, Huan-Huan1; Bian, Huan1; Hong, Ying-Ying2; Wang, Yi-Xiang3; Guo, Chuan-Bin1
关键词Oral cancer lymphatic metastasis animal model oral lymphatic system homologous cell pair
刊名INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY
2014
7期:4页:1563-1571
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology ; Pathology
研究领域[WOS]Oncology ; Pathology
关键词[WOS]PROMOTES TUMOR-METASTASIS ; GENE DELIVERY ; MOUSE MODEL ; CARCINOMA ; CANCER ; LYMPHANGIOGENESIS ; EXPRESSION ; TONGUE ; INVASION ; GROWTH
英文摘要

The purpose of this study is to establish in vivo and in vitro models for studying lymphatic metastasis of squamous cell carcinoma (SCC). Three cell lines CAL-27, Tca-83, and HeLa were injected into the tongue of nude mice. Forty days after injection, we could isolate cells of 2 homologous cell lines LN-CAL-27 and LN-HeLa from lymph node metastasis lesions. Then, the homologous cell pairs were compared by the CCK-8 assay, wound healing assay, real-time PCR, western blot, and animal experiments. The results showed that all the three cell lines could be used to establish lymphatic metastasis animal models, and the lymphatic metastasis process was observed clearly. In addition, the homologous cell pairs performed differently from parent lines with respect to biological behavior and lymphatic metastasis-related gene and protein expression. In conclusion, CAL-27, Tca-83, and HeLa cells could be used to simulate the lymphatic metastasis process of oral cancer in vivo. Furthermore, the homologous cell pairs (CAL-27 and LN-CAL-27; HeLa and LN-HeLa) are potential tools for in vitro investigation of the mechanisms underlying metastasis.

语种英语
WOS记录号WOS:000335227600029
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61455
专题北京大学口腔医学院_口腔病理科
北京大学口腔医学院_中心实验室
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Dept Pathol, Beijing 100081, Peoples R China
3.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Pu, Yin-Fei,Wang, Lin,Wu, Huan-Huan,et al. Generation of homologous cell pairs using the oral lymphatic system[J]. INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,2014,7(4):1563-1571.
APA Pu, Yin-Fei.,Wang, Lin.,Wu, Huan-Huan.,Bian, Huan.,Hong, Ying-Ying.,...&Guo, Chuan-Bin.(2014).Generation of homologous cell pairs using the oral lymphatic system.INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY,7(4),1563-1571.
MLA Pu, Yin-Fei,et al."Generation of homologous cell pairs using the oral lymphatic system".INTERNATIONAL JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOGY 7.4(2014):1563-1571.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Pu, Yin-Fei]的文章
[Wang, Lin]的文章
[Wu, Huan-Huan]的文章
百度学术
百度学术中相似的文章
[Pu, Yin-Fei]的文章
[Wang, Lin]的文章
[Wu, Huan-Huan]的文章
必应学术
必应学术中相似的文章
[Pu, Yin-Fei]的文章
[Wang, Lin]的文章
[Wu, Huan-Huan]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。