IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Expression of programmed cell death 5 gene involves in regulation of apoptosis in gastric tumor cells
Yang, Y. -H.; Zhao, M.; Li, W. -M.; Chen, Y. -Y.; Kang, B.; Lu, Y. -Y.
关键词apoptosis gastric cancer nuclear translocation programmed cell death 5
刊名APOPTOSIS
2006-06-01
DOI10.1007/s10495-006-6714-6
11期:6页:993-1001
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]CANCER ; TFAR19 ; LIVER ; CARCINOMA ; PROFILES ; PROTEIN
英文摘要

The protein of programmed cell death 5 (PDCD5) is believed to participate in regulation of apoptosis. Although PDCD5 is reducibly expressed in various human tumors, it is not clear which expression level of PDCD5 is in gastric cancer (GC). In this study, we have systematically employed the approaches of RT-PCR, Real- time PCR, Immunohistochemistry (IHC), Immunofluorescence staining (IFS) and Western blot to determine the PDCD5 expression in GC cells and primary tumors, at mRNA and protein level, respectively. Our data revealed that the positive rate of PDCD5 expression in the gastric tumor tissues was significantly less than that of the normal tissues (14 out of 102 vs 36 out of 51), whereas, the decreased expression of PDCD5 protein was well correlated with the up-regulated expression of Bcl-2 in these tissues, and the up-regulated expression and nuclear translocation of PDCD5 protein were verified in the apoptotic GC cells induced by Diallyl trisulfide (DATS). Furthermore, the survival curve has suggested that the more PDCD5 expressions were found in the patients, the longer the survival periods were. Therefore, our observations lay down a reasonable postulation that PDCD5 may play a key role to regulate the apoptotic processes in the GC cells and gastric tumors.

语种英语
WOS记录号WOS:000238154300011
Citation statistics
Cited Times:53[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61469
Collection北京大学临床肿瘤学院
作者单位1.Peking Univ, Sch Oncol, Beijing Inst Canc Res, Beijing Mol Oncol Lab, Beijing 100036, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Lab Med Immunol, Beijing 100083, Peoples R China
3.Beijing Airport Ind Zone B 6, Chinese Acad Sci, Beijing Genom Inst, Beijing 101300, Peoples R China
Recommended Citation
GB/T 7714
Yang, Y. -H.,Zhao, M.,Li, W. -M.,et al. Expression of programmed cell death 5 gene involves in regulation of apoptosis in gastric tumor cells[J]. APOPTOSIS,2006,11(6):993-1001.
APA Yang, Y. -H.,Zhao, M.,Li, W. -M.,Chen, Y. -Y.,Kang, B.,&Lu, Y. -Y..(2006).Expression of programmed cell death 5 gene involves in regulation of apoptosis in gastric tumor cells.APOPTOSIS,11(6),993-1001.
MLA Yang, Y. -H.,et al."Expression of programmed cell death 5 gene involves in regulation of apoptosis in gastric tumor cells".APOPTOSIS 11.6(2006):993-1001.
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