|Formaldehyde increases intracellular calcium concentration in primary cultured hippocampal neurons partly through NMDA receptors and T-type calcium channels|
|Chi, Ye-Nan1,2; Zhang, Xu1,2; Cai, Jie1,2; Liu, Feng-Yu1,2; Xing, Guo-Gang3; Wan, You1,2,3|
|关键词||formaldehyde intracellular calcium neuronal activation NMDA receptors T-type calcium channels|
|WOS标题词||Science & Technology|
|研究领域[WOS]||Neurosciences & Neurology|
|关键词[WOS]||THERAPEUTIC TARGETS ; PARKINSONS-DISEASE ; FORMALIN ; MODEL ; PAIN ; TAU|
Formaldehyde at high concentrations is a contributor to air pollution. It is also an endogenous metabolic product in cells, and when beyond physiological concentrations, has pathological effects on neurons. Formaldehyde induces mis-folding and aggregation of neuronal tau protein, hippocampal neuronal apoptosis, cognitive impairment and loss of memory functions, as well as excitation of peripheral nociceptive neurons in cancer pain models. Intracellular calcium ([Ca2+](i)) is an important intracellular messenger, and plays a key role in many pathological processes. The present study aimed to investigate the effect of formaldehyde on [Ca2+](i) and the possible involvement of N-methyl-D-aspartate receptors (NMDARs) and T-type Ca2+ channels on the cell membrane.
Using primary cultured hippocampal neurons as a model, changes of [Ca2+](i) in the presence of formaldehyde at a low concentration were detected by confocal laser scanning microscopy.
Formaldehyde at 1 mmol/L approximately doubled [Ca2+](i). (2R)-amino-5-phosphonopentanoate (AP5, 25 mu mol/L, an NMDAR antagonist) and mibefradil (MIB, 1 mu mol/L, a T-type Ca2+ channel blocker), given 5 min after formaldehyde perfusion, each partly inhibited the formaldehyde-induced increase of [Ca2+](i), and this inhibitory effect was reinforced by combined application of AP5 and MIB. When applied 3 min before formaldehyde perfusion, AP5 (even at 50 mu mol/L) did not inhibit the formaldehyde-induced increase of [Ca2+](i), but MIB (1 mu mol/L) significantly inhibited this increase by 70%.
These results suggest that formaldehyde at a low concentration increases [Ca2+](i) in cultured hippocampal neurons; NMDARs and T-type Ca2+ channels may be involved in this process.
|项目编号||81171042 ; 81070893 ; 81221002 ; 20110001110058|
|资助机构||National Natural Science Foundation of China ; Beijing Outstanding Ph.D. Program Mentor Grant ; Specialized Research Fund for Doctoral Programs of Higher Education, China|
|作者单位||1.Peking Univ, Neurosci Res Inst, Beijing 100191, Peoples R China|
2.Peking Univ, Dept Neurobiol, Beijing 100191, Peoples R China
3.Peking Univ, Minist Hlth, Minist Educ, Key Lab Neurosci, Beijing 100191, Peoples R China
|Chi, Ye-Nan,Zhang, Xu,Cai, Jie,et al. Formaldehyde increases intracellular calcium concentration in primary cultured hippocampal neurons partly through NMDA receptors and T-type calcium channels[J]. NEUROSCIENCE BULLETIN,2012,28(6):715-722.|
|APA||Chi, Ye-Nan,Zhang, Xu,Cai, Jie,Liu, Feng-Yu,Xing, Guo-Gang,&Wan, You.(2012).Formaldehyde increases intracellular calcium concentration in primary cultured hippocampal neurons partly through NMDA receptors and T-type calcium channels.NEUROSCIENCE BULLETIN,28(6),715-722.|
|MLA||Chi, Ye-Nan,et al."Formaldehyde increases intracellular calcium concentration in primary cultured hippocampal neurons partly through NMDA receptors and T-type calcium channels".NEUROSCIENCE BULLETIN 28.6(2012):715-722.|
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