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学科主题: 基础医学
题名:
Formaldehyde increases intracellular calcium concentration in primary cultured hippocampal neurons partly through NMDA receptors and T-type calcium channels
作者: Chi, Ye-Nan1,2; Zhang, Xu1,2; Cai, Jie1,2; Liu, Feng-Yu1,2; Xing, Guo-Gang3; Wan, You1,2,3
关键词: formaldehyde ; intracellular calcium ; neuronal activation ; NMDA receptors ; T-type calcium channels
刊名: NEUROSCIENCE BULLETIN
发表日期: 2012-12-01
DOI: 10.1007/s12264-012-1284-9
卷: 28, 期:6, 页:715-722
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: THERAPEUTIC TARGETS ; PARKINSONS-DISEASE ; FORMALIN ; MODEL ; PAIN ; TAU
英文摘要:

Formaldehyde at high concentrations is a contributor to air pollution. It is also an endogenous metabolic product in cells, and when beyond physiological concentrations, has pathological effects on neurons. Formaldehyde induces mis-folding and aggregation of neuronal tau protein, hippocampal neuronal apoptosis, cognitive impairment and loss of memory functions, as well as excitation of peripheral nociceptive neurons in cancer pain models. Intracellular calcium ([Ca2+](i)) is an important intracellular messenger, and plays a key role in many pathological processes. The present study aimed to investigate the effect of formaldehyde on [Ca2+](i) and the possible involvement of N-methyl-D-aspartate receptors (NMDARs) and T-type Ca2+ channels on the cell membrane.

Using primary cultured hippocampal neurons as a model, changes of [Ca2+](i) in the presence of formaldehyde at a low concentration were detected by confocal laser scanning microscopy.

Formaldehyde at 1 mmol/L approximately doubled [Ca2+](i). (2R)-amino-5-phosphonopentanoate (AP5, 25 mu mol/L, an NMDAR antagonist) and mibefradil (MIB, 1 mu mol/L, a T-type Ca2+ channel blocker), given 5 min after formaldehyde perfusion, each partly inhibited the formaldehyde-induced increase of [Ca2+](i), and this inhibitory effect was reinforced by combined application of AP5 and MIB. When applied 3 min before formaldehyde perfusion, AP5 (even at 50 mu mol/L) did not inhibit the formaldehyde-induced increase of [Ca2+](i), but MIB (1 mu mol/L) significantly inhibited this increase by 70%.

These results suggest that formaldehyde at a low concentration increases [Ca2+](i) in cultured hippocampal neurons; NMDARs and T-type Ca2+ channels may be involved in this process.

语种: 英语
所属项目编号: 81171042 ; 81070893 ; 81221002 ; 20110001110058
项目资助者: National Natural Science Foundation of China ; Beijing Outstanding Ph.D. Program Mentor Grant ; Specialized Research Fund for Doctoral Programs of Higher Education, China
WOS记录号: WOS:000312343200006
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61472
Appears in Collections:基础医学院_神经生物学系_期刊论文

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作者单位: 1.Peking Univ, Neurosci Res Inst, Beijing 100191, Peoples R China
2.Peking Univ, Dept Neurobiol, Beijing 100191, Peoples R China
3.Peking Univ, Minist Hlth, Minist Educ, Key Lab Neurosci, Beijing 100191, Peoples R China

Recommended Citation:
Chi, Ye-Nan,Zhang, Xu,Cai, Jie,et al. Formaldehyde increases intracellular calcium concentration in primary cultured hippocampal neurons partly through NMDA receptors and T-type calcium channels[J]. NEUROSCIENCE BULLETIN,2012,28(6):715-722.
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