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学科主题基础医学
Formaldehyde increases intracellular calcium concentration in primary cultured hippocampal neurons partly through NMDA receptors and T-type calcium channels
Chi, Ye-Nan1,2; Zhang, Xu1,2; Cai, Jie1,2; Liu, Feng-Yu1,2; Xing, Guo-Gang3; Wan, You1,2,3
关键词formaldehyde intracellular calcium neuronal activation NMDA receptors T-type calcium channels
刊名NEUROSCIENCE BULLETIN
2012-12-01
DOI10.1007/s12264-012-1284-9
28期:6页:715-722
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]THERAPEUTIC TARGETS ; PARKINSONS-DISEASE ; FORMALIN ; MODEL ; PAIN ; TAU
英文摘要

Formaldehyde at high concentrations is a contributor to air pollution. It is also an endogenous metabolic product in cells, and when beyond physiological concentrations, has pathological effects on neurons. Formaldehyde induces mis-folding and aggregation of neuronal tau protein, hippocampal neuronal apoptosis, cognitive impairment and loss of memory functions, as well as excitation of peripheral nociceptive neurons in cancer pain models. Intracellular calcium ([Ca2+](i)) is an important intracellular messenger, and plays a key role in many pathological processes. The present study aimed to investigate the effect of formaldehyde on [Ca2+](i) and the possible involvement of N-methyl-D-aspartate receptors (NMDARs) and T-type Ca2+ channels on the cell membrane.

Using primary cultured hippocampal neurons as a model, changes of [Ca2+](i) in the presence of formaldehyde at a low concentration were detected by confocal laser scanning microscopy.

Formaldehyde at 1 mmol/L approximately doubled [Ca2+](i). (2R)-amino-5-phosphonopentanoate (AP5, 25 mu mol/L, an NMDAR antagonist) and mibefradil (MIB, 1 mu mol/L, a T-type Ca2+ channel blocker), given 5 min after formaldehyde perfusion, each partly inhibited the formaldehyde-induced increase of [Ca2+](i), and this inhibitory effect was reinforced by combined application of AP5 and MIB. When applied 3 min before formaldehyde perfusion, AP5 (even at 50 mu mol/L) did not inhibit the formaldehyde-induced increase of [Ca2+](i), but MIB (1 mu mol/L) significantly inhibited this increase by 70%.

These results suggest that formaldehyde at a low concentration increases [Ca2+](i) in cultured hippocampal neurons; NMDARs and T-type Ca2+ channels may be involved in this process.

语种英语
WOS记录号WOS:000312343200006
项目编号81171042 ; 81070893 ; 81221002 ; 20110001110058
资助机构National Natural Science Foundation of China ; Beijing Outstanding Ph.D. Program Mentor Grant ; Specialized Research Fund for Doctoral Programs of Higher Education, China
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61472
专题北京大学基础医学院_神经生物学系
北京大学基础医学院
作者单位1.Peking Univ, Neurosci Res Inst, Beijing 100191, Peoples R China
2.Peking Univ, Dept Neurobiol, Beijing 100191, Peoples R China
3.Peking Univ, Minist Hlth, Minist Educ, Key Lab Neurosci, Beijing 100191, Peoples R China
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GB/T 7714
Chi, Ye-Nan,Zhang, Xu,Cai, Jie,et al. Formaldehyde increases intracellular calcium concentration in primary cultured hippocampal neurons partly through NMDA receptors and T-type calcium channels[J]. NEUROSCIENCE BULLETIN,2012,28(6):715-722.
APA Chi, Ye-Nan,Zhang, Xu,Cai, Jie,Liu, Feng-Yu,Xing, Guo-Gang,&Wan, You.(2012).Formaldehyde increases intracellular calcium concentration in primary cultured hippocampal neurons partly through NMDA receptors and T-type calcium channels.NEUROSCIENCE BULLETIN,28(6),715-722.
MLA Chi, Ye-Nan,et al."Formaldehyde increases intracellular calcium concentration in primary cultured hippocampal neurons partly through NMDA receptors and T-type calcium channels".NEUROSCIENCE BULLETIN 28.6(2012):715-722.
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