IR@PKUHSC  > 北京大学药学院
学科主题药学
Photocaging Strategy for Functionalisation of Oligonucleotides and Its Applications for Oligonucleotide Labelling and Cyclisation
Su, Meng; Wang, Jie; Tang, XinJing
关键词caged compounds oligonucleotide modifiers oligonucleotides photoactivation photolabile phosphoramidites
刊名CHEMISTRY-A EUROPEAN JOURNAL
2012-07-01
DOI10.1002/chem.201103833
18期:31页:9628-9637
收录类别SCI ; IC
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Multidisciplinary
资助者National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; Program for New Century Excellent Talents in University ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; Program for New Century Excellent Talents in University
研究领域[WOS]Chemistry
关键词[WOS]PHASE SYNTHESIS SUPPORTS ; PROTECTED OLIGONUCLEOTIDES ; CARBOXYLIC-ACIDS ; SOLID SUPPORT ; DNA ; OLIGODEOXYNUCLEOTIDES ; AMINES ; PROBES ; HYBRIDIZATION ; CONVERGENT
英文摘要

We have used a photocaging strategy to develop novel phosphoramidites and expand the repertoire of protecting groups for modification of oligonucleotides by solid-phase synthesis. We synthesised five photolabile phosphoramidites and four new photolabile controlled pore glasses (CPGs). By using these photolabile phosphoramidites and CPGs, modified oligodeoxynucleotides (ODNs) with phosphate, amine, acid, thiol and carbonyl moieties at 5′ and/or 3′ ends were readily synthesised. To the best of our knowledge, this is the first report of introducing a carbonyl at the 5′ end and thiol groups at both ends of ODNs with photolabile modifiers. Terminal labelling was also easily realised in solution or by on-column solid-phase synthesis. By using the photolabile amine modifier and the photolabile acid CPG, cyclisation of an oligodeoxynucleotide was achieved with good yields. This study provides an alternative way to introduce functional groups into oligonucleotides and expand the scope of oligonucleotide bio-orthogonal labelling.

语种英语
所属项目编号21072015 ; 2012CB720600 ; NCET-10-0203
资助者National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; Program for New Century Excellent Talents in University ; National Natural Science Foundation of China ; National Basic Research Program of China (973 Program) ; Program for New Century Excellent Talents in University
WOS记录号WOS:000306670200025
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61475
专题北京大学药学院
作者单位Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Su, Meng,Wang, Jie,Tang, XinJing. Photocaging Strategy for Functionalisation of Oligonucleotides and Its Applications for Oligonucleotide Labelling and Cyclisation[J]. CHEMISTRY-A EUROPEAN JOURNAL,2012,18(31):9628-9637.
APA Su, Meng,Wang, Jie,&Tang, XinJing.(2012).Photocaging Strategy for Functionalisation of Oligonucleotides and Its Applications for Oligonucleotide Labelling and Cyclisation.CHEMISTRY-A EUROPEAN JOURNAL,18(31),9628-9637.
MLA Su, Meng,et al."Photocaging Strategy for Functionalisation of Oligonucleotides and Its Applications for Oligonucleotide Labelling and Cyclisation".CHEMISTRY-A EUROPEAN JOURNAL 18.31(2012):9628-9637.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Su, Meng]的文章
[Wang, Jie]的文章
[Tang, XinJing]的文章
百度学术
百度学术中相似的文章
[Su, Meng]的文章
[Wang, Jie]的文章
[Tang, XinJing]的文章
必应学术
必应学术中相似的文章
[Su, Meng]的文章
[Wang, Jie]的文章
[Tang, XinJing]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。