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学科主题: 临床医学
题名:
Electrical Stimulation Promotes Regeneration of Defective Peripheral Nerves after Delayed Repair Intervals Lasting under One Month
作者: Xu, Chungui; Kou, Yuhui; Zhang, Peixun; Han, Na; Yin, Xiaofeng; Deng, Jiuxu; Chen, Bo; Jiang, Baoguo
刊名: PLOS ONE
发表日期: 2014-09-02
DOI: 10.1371/journal.pone.0105045
卷: 9, 期:9
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: SCHWANN-CELL DENERVATION ; RAT FEMORAL MOTONEURONS ; NEUROTROPHIC FACTOR ; MUSCLE REINNERVATION ; AXONAL REGENERATION ; SPINAL MOTONEURONS ; GENE-EXPRESSION ; MOTOR-NEURONS ; ADULT RATS ; IN-VIVO
英文摘要:

Background: Electrical stimulation (ES) has been proven to be an effective means of enhancing the speed and accuracy of nerve regeneration. However, these results were recorded when the procedure was performed almost immediately after nerve injury. In clinical settings, most patients cannot be treated immediately. Some patients with serious trauma or contaminated wounds need to wait for nerve repair surgery. Delays in nerve repair have been shown to be associated with poorer results than immediate surgery. It is not clear whether electrical stimulation still has any effect on nerve regeneration after enough time has elapsed.

Methods: A delayed nerve repair model in which the rats received delayed nerve repair after 1 day, 1 week, 1 month, and 2 months was designed. At each point in time, the nerve stumps of half the rats were bridged with an absorbable conduit and the rats were given 1 h of weak electrical stimulation. The other half was not treated. In order to analyzethe morphological and molecular differences among these groups, 6 ES rats and 6 sham ES rats per point in time were killed 5 days after surgery. The other rats in each group were allowed to recover for 6 weeks before the final functional test and tissue observation.

Results: The amounts of myelinated fibers in the distal nerve stumps decreased as the delay in repair increased for both ES rats and sham ES rats. In the 1-day-delay and 1-week-delay groups, there were more fibers in ES rats than in sham ES rats. And the compound muscle action potential (CMAP) and motor nerve conduction velocity (MNCV) results were better for ES rats in these two groups. In order to analyze the mechanisms underlying these differences, Masson staining was performed on the distal nerves and quantitative PCR on the spinal cords. Results showed that, after delays in repair of 1 month and 2 months, there was more collagen tissue hyperplasia in the distal nerve in all rats. The brain-derived neurotrophic factor (BDNF) and trkB expression levels in the spinal cords of ES rats were higher than in sham ES rats. However, these differences decreased as the delay in repair increased.

Conclusions: Electrical stimulation does not continue to promote nerve regeneration after long delays in nerve repair. The effective interval for nerve regeneration after delayed repair was found to be less than 1 month. The mechanism seemed to be related to the expression of nerve growth factors and regeneration environment in the distal nerves.

语种: 英语
所属项目编号: 2014CB542200 ; 31271284 ; 31171150 ; 81171146 ; 30971526 ; 31100860 ; 31040043 ; BMU20110270 ; A-2008-10
项目资助者: Chinese National Ministry of Science and Technology 973 Project Planning ; National Natural Science Fund ; Educational Ministry New Century Excellent Talents Support Project ; Beijing City Science and Technology New Star Classification
WOS记录号: WOS:000341231500016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61480
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: Peking Univ, Dept Trauma & Orthopaed, Peoples Hosp, Beijing 100871, Peoples R China

Recommended Citation:
Xu, Chungui,Kou, Yuhui,Zhang, Peixun,et al. Electrical Stimulation Promotes Regeneration of Defective Peripheral Nerves after Delayed Repair Intervals Lasting under One Month[J]. PLOS ONE,2014,9(9).
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