北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 期刊论文
学科主题: 临床医学
题名:
FAM3A is a target gene of peroxisome proliferator-activated receptor gamma
作者: Zhou, Yanlan; Jia, Shi; Wang, Chunjiong; Chen, Zhenzhen; Chi, Yujing1; Li, Jing2; Xu, Guoheng3,4; Guan, Youfei3,4; Yang, Jichun3,4
关键词: FAM3A ; Nuclear receptor ; PPAR gamma ; PPRE ; Akt
刊名: BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
发表日期: 2013-08-01
DOI: 10.1016/j.bbagen.2013.03.029
卷: 1830, 期:8, 页:4160-4170
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]: PANCREATIC-DERIVED FACTOR ; FATTY LIVER IMPROVEMENT ; LEPTIN-DEFICIENT MICE ; PPAR-GAMMA ; FACTOR PANDER ; HEPATIC STEATOSIS ; BETA-CELLS ; NONALCOHOLIC STEATOHEPATITIS ; FASTING HYPERGLYCEMIA ; INSULIN-RESISTANCE
英文摘要:

Background: To date, the biological function of FAM3A, the first member of FAM3 gene family, remains unknown. We aimed to investigate whether the expression of FAM3A in liver cells is regulated by peroxisome proliferator-activated receptors (PPARs).

Methods and results: The transcriptional activity of human and mouse FAM3A gene promoters was determined by luciferase reporter assay system. PPAR gamma agonist rosiglitazone induced FAM3A expression in primary cultured mouse hepatocytes and human HepG2 cells. PPAR gamma antagonism blocked rosiglitazone-induced FAM3A expression, whereas PPAR gamma overexpression stimulated FAM3A expression in HepG2 cells. In contrast PPAR alpha agonist fenofibrate or PPAR beta agonist GW0742 failed to affect FAM3A expression in HepG2 cells. The transcriptional activities of human and mouse FAM3A promoters were markedly stimulated by PPAR gamma activation, but not by PPAR alpha and PPAR beta activation. Chromatin immunoprecipitation (ChIP) assay revealed a direct binding of PPAR gamma to the putative peroxisome proliferator response element (PPRE) located at -1258/-1246 in the human FAM3A promoter. Site-directed mutagenesis of this PPRE-like motif abolished PPAR gamma′s stimulatory effect on the transcriptional activity of human FAM3A promoter. In vivo, oral rosiglitazone treatment upregulated FAM3A expression in the livers of C57BL/6 mice and db/db mice. Moreover, upregulation of FAM3A by PPAR gamma activation was correlated with increased level of phosphorylated Akt (pAkt) in liver cells.

Conclusions: FAM3A as a novel target gene of PPAR gamma. Upregulation of FAM3A by PPAR gamma activation is correlated with increased pAkt level in liver cells.

General significance: Upregulation of FAM3A might contribute to PPAR gamma′s metabolic effects in the liver. (c) 2013 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 2012CB517504 ; 2009CB941603 ; 81170791/30870905/2011ZX09102/81200625 ; 7122107
项目资助者: Ministry of Science and Technology ; Natural Science Foundation ; Beijing Natural Science Foundation
WOS记录号: WOS:000320896100016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61494
Appears in Collections:北京大学第二临床医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ Peoples Hosp, Ctr Lab, Beijing, Peoples R China
2.Peking Beijing Univ Diabet Ctr, Beijing, Peoples R China
3.Peking Univ Peoples Hosp, Dept Gastroenterol, Beijing, Peoples R China
4.Peking Beijing Univ Hlth Sci Ctr, Key Lab Mol Cardiovasc Sci, Beijing 100191, Peoples R China

Recommended Citation:
Zhou, Yanlan,Jia, Shi,Wang, Chunjiong,et al. FAM3A is a target gene of peroxisome proliferator-activated receptor gamma[J]. BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS,2013,1830(8):4160-4170.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhou, Yanlan]'s Articles
[Jia, Shi]'s Articles
[Wang, Chunjiong]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhou, Yanlan]‘s Articles
[Jia, Shi]‘s Articles
[Wang, Chunjiong]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace