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学科主题: 临床医学
题名:
Biological characteristics of human adipose-derived stem cells and their response to periostin in vitro
作者: Li Ying1; Yang Xin1; Nie Fang-fei1; Zhao Xia1; Qin Ze-lian2; Li Jian-ning1
关键词: adipose-derived stem cell ; differentiation ; periostin ; function
刊名: CHINESE MEDICAL JOURNAL
发表日期: 2013-04-20
DOI: 10.3760/cma.j.issn.0366-6999.20122674
卷: 126, 期:8, 页:1491-1497
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, General & Internal
研究领域[WOS]: General & Internal Medicine
关键词[WOS]: GENE-EXPRESSION ; STROMAL CELLS ; BONE-MARROW ; TISSUE ; CANCER ; ADHESION ; INVASION ; PROTEIN
英文摘要:

Background Many studies on periostin have focused on its role in tumors and vascular reconstruction. However, the effect of periostin on stem cell function remains unclear. The aim of this study was to enhance vitality in adipose-derived stem cells (ADSCs), the effect of periostin on the function of ADSCs was observed.

Methods Human ADSCs (hADSCs) were isolated from human adipose tissue by collagenase I digestion and collected in multi-periods for in vitro culture. CD29, CD34, CD44, CD45 and CD105 were detected by flow cytometry. In addition, directed differentiation of hADSCs was induced using adipogenic, osteogenic and chondrogenic induction mediums. The induced morphological changes were observed using oil red 0, Alizarin red and alcian blue staining. Periostin was administered to hADSCs in an acidic environment. The treatments of cells were divided into three groups: a periostin group (P); an acidic control group (A); a normal group (N). Then the resulting cell proliferation and migration were detected using a Cell Counting Kit-8 (CCK-8) and a transwell chamber assay, respectively.

Results The detection rates of CD29, CD44, CD105, CD34 and CD45 were 98.89%, 93.73%, 86.99%, 0.19% and 0.16%. The specific staining of cells was positive after induction culture. The mean absorbance of the cells in group P and A at 12 hours were 16.67% and 22.22% greater than group N, respectively (P <0.01). The mean absorbance of cells from group P was 20.00% greater than that of group A at 48 hours (P <0.05). The mean number of migratory cells per visual field in group A was 50.38% lower than that in group N (P <0.05). The migratory cell number in group P was 119.98% greater than that in group A (P <0.05).

Conclusions The acidic environment impacted hADSC proliferation and inhibited cell migration. However, periostin was able to promote the proliferation and migration of hADSCs despite the acidic environment.

语种: 英语
所属项目编号: 30672186 ; 30973126
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000319529000017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61527
Appears in Collections:北京大学第三临床医学院_成形外科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Dept Plast Surg, Beijing 100191, Peoples R China
2.Peking Univ, Hosp 3, Med Res Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Li Ying,Yang Xin,Nie Fang-fei,et al. Biological characteristics of human adipose-derived stem cells and their response to periostin in vitro[J]. CHINESE MEDICAL JOURNAL,2013,126(8):1491-1497.
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