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学科主题: 临床医学
题名:
Gene expression patterns in the histopathological classification of epithelial ovarian cancer
作者: Zhu, Honglan1,2,3; Yu, Jing Jie1,2
关键词: gene signature ; histopathological types ; epithelial ovarian cancer ; real-time quantitative PCR
刊名: EXPERIMENTAL AND THERAPEUTIC MEDICINE
发表日期: 2010
DOI: 10.3892/etm_00000030
卷: 1, 期:1, 页:187-192
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental
研究领域[WOS]: Research & Experimental Medicine
关键词[WOS]: MESSENGER-RNA LEVELS ; SURFACE EPITHELIUM ; CLEAR-CELL ; TUMORIGENESIS ; TUMORS ; PATHWAYS ; FAMILY ; ALTERS ; ERCC1 ; UBC9
英文摘要:

The purpose of this study was to screen cancerrelated genes and to identify histopathological gene expression I patterns as potential btomarkers in human epithelial ovarian 1 cancer (EOC) Fifty genes were screened by reversetranscription polymerase chain reaction assay with cDNA from 83 EOC tissues and 48 normal ovarian specimens of ovarian cancer patients and evaluated by gel electrophoresis analysis Twenty expressed genes were assessed by real-time relativequantity (RQ)-PCR in 30 EOC specimens for gene signature study Four genes, TAL2, EGF, ILF3 and UBE2I, were I investigated for gene expression patterns in histopathological classification of EOC RQ-value (Ct, Delta Ct, Delta Delta Ct, RQ and gene expression plots) was generated by ABI 7500 Fast System SDS Software (version 1 4) SPSS 15 0 software was used for statistical analysis Using real-time RQ-PCR, we found that iAL2,EGF ILF3 and UBE2I demonstrated distinct expression 1 patterns in histological types of epithelial ovarian cancer The ekpression of ILF3 and UBE2I in tumors was significantly higher than in normal tissue, with extremely high expression in serous carcinomas compared to mucinous, endometrium an, d clear cell carcinomas In addition ILF3 and UBE2I were Overexpressed in advanced stage and advanced grade ovarian 1 cancer, compared to early stage or well-differentiated ovarian cancer This is the first report of TAL2 and /LF3 expression 1 in the normal human ovary and epithelial ovarian cancer Our results indicate that overexpression of ILF3 and UBE2I in advanced stage and advanced grade suggest lhat these two genes may play an important role in tumongenesis/tumor progression and pathological differentiation of the disease Notably, ILF3 plays a role in DNA binding activity and transcriptional and post-transcriptional regulation, UBE2I is required in ubiquitination and sumoylation and is involved in DNA repair and apoptosis of cells Further investigations to reveal the molecular mechanisms related to the activation of ILF3 and UBE2I in the development of EOC are warranted

语种: 英语
所属项目编号: R03 CA107979-01
项目资助者: NIH ; R03 CA107979-01 and the WVU Mary Babb Randolph Cancer Center,
WOS记录号: WOS:000284562100030
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61548
Appears in Collections:北京大学第二临床医学院_期刊论文

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作者单位: 1.W Virginia Univ, Dept Biochem, Dept Basic Pharmaceut Sci, Sch Med,Sch Pharm,Robert C Byrd Hlth Sci Ctr, Morgantown, WV 26506 USA
2.W Virginia Univ, Mary Babb Randolph Canc Ctr, Robert C Byrd Hlth Sci Ctr, Morgantown, WV 26506 USA
3.Peking Univ, Peoples Hosp, Dept Obstet & Gynecol, Beijing 100044, Peoples R China

Recommended Citation:
Zhu, Honglan,Yu, Jing Jie. Gene expression patterns in the histopathological classification of epithelial ovarian cancer[J]. EXPERIMENTAL AND THERAPEUTIC MEDICINE,2010,1(1):187-192.
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