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Gene expression patterns in the histopathological classification of epithelial ovarian cancer
Zhu, Honglan1,2,3; Yu, Jing Jie1,2
关键词gene signature histopathological types epithelial ovarian cancer real-time quantitative PCR
刊名EXPERIMENTAL AND THERAPEUTIC MEDICINE
2010
DOI10.3892/etm_00000030
1期:1页:187-192
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, Research & Experimental
研究领域[WOS]Research & Experimental Medicine
关键词[WOS]MESSENGER-RNA LEVELS ; SURFACE EPITHELIUM ; CLEAR-CELL ; TUMORIGENESIS ; TUMORS ; PATHWAYS ; FAMILY ; ALTERS ; ERCC1 ; UBC9
英文摘要

The purpose of this study was to screen cancerrelated genes and to identify histopathological gene expression I patterns as potential btomarkers in human epithelial ovarian 1 cancer (EOC) Fifty genes were screened by reversetranscription polymerase chain reaction assay with cDNA from 83 EOC tissues and 48 normal ovarian specimens of ovarian cancer patients and evaluated by gel electrophoresis analysis Twenty expressed genes were assessed by real-time relativequantity (RQ)-PCR in 30 EOC specimens for gene signature study Four genes, TAL2, EGF, ILF3 and UBE2I, were I investigated for gene expression patterns in histopathological classification of EOC RQ-value (Ct, Delta Ct, Delta Delta Ct, RQ and gene expression plots) was generated by ABI 7500 Fast System SDS Software (version 1 4) SPSS 15 0 software was used for statistical analysis Using real-time RQ-PCR, we found that iAL2,EGF ILF3 and UBE2I demonstrated distinct expression 1 patterns in histological types of epithelial ovarian cancer The ekpression of ILF3 and UBE2I in tumors was significantly higher than in normal tissue, with extremely high expression in serous carcinomas compared to mucinous, endometrium an, d clear cell carcinomas In addition ILF3 and UBE2I were Overexpressed in advanced stage and advanced grade ovarian 1 cancer, compared to early stage or well-differentiated ovarian cancer This is the first report of TAL2 and /LF3 expression 1 in the normal human ovary and epithelial ovarian cancer Our results indicate that overexpression of ILF3 and UBE2I in advanced stage and advanced grade suggest lhat these two genes may play an important role in tumongenesis/tumor progression and pathological differentiation of the disease Notably, ILF3 plays a role in DNA binding activity and transcriptional and post-transcriptional regulation, UBE2I is required in ubiquitination and sumoylation and is involved in DNA repair and apoptosis of cells Further investigations to reveal the molecular mechanisms related to the activation of ILF3 and UBE2I in the development of EOC are warranted

语种英语
WOS记录号WOS:000284562100030
项目编号R03 CA107979-01
资助机构NIH ; R03 CA107979-01 and the WVU Mary Babb Randolph Cancer Center,
引用统计
被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61548
专题北京大学第二临床医学院
北京大学第二临床医学院_妇科
作者单位1.W Virginia Univ, Dept Biochem, Dept Basic Pharmaceut Sci, Sch Med,Sch Pharm,Robert C Byrd Hlth Sci Ctr, Morgantown, WV 26506 USA
2.W Virginia Univ, Mary Babb Randolph Canc Ctr, Robert C Byrd Hlth Sci Ctr, Morgantown, WV 26506 USA
3.Peking Univ, Peoples Hosp, Dept Obstet & Gynecol, Beijing 100044, Peoples R China
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Zhu, Honglan,Yu, Jing Jie. Gene expression patterns in the histopathological classification of epithelial ovarian cancer[J]. EXPERIMENTAL AND THERAPEUTIC MEDICINE,2010,1(1):187-192.
APA Zhu, Honglan,&Yu, Jing Jie.(2010).Gene expression patterns in the histopathological classification of epithelial ovarian cancer.EXPERIMENTAL AND THERAPEUTIC MEDICINE,1(1),187-192.
MLA Zhu, Honglan,et al."Gene expression patterns in the histopathological classification of epithelial ovarian cancer".EXPERIMENTAL AND THERAPEUTIC MEDICINE 1.1(2010):187-192.
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