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学科主题: 基础医学
题名:
ISL1 Promotes Pancreatic Islet Cell Proliferation
作者: Guo, Ting; Wang, Weiping; Zhang, Hui; Liu, Yinan; Chen, Ping; Ma, Kangtao; Zhou, Chunyan
刊名: PLOS ONE
发表日期: 2011-08-04
DOI: 10.1371/journal.pone.0022387
卷: 6, 期:8
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Multidisciplinary Sciences
研究领域[WOS]: Science & Technology - Other Topics
关键词[WOS]: HOMEOBOX GENE ISL-1 ; BETA-CELLS ; ENDOCRINE PANCREAS ; BINDING-PROTEIN ; EXPRESSION ; TRANSCRIPTION ; DOMAIN ; ADULT ; HOMEODOMAIN ; P53
英文摘要:

Background: Islet 1 (ISL1), a LIM-homeodomain transcription factor is essential for promoting pancreatic islets proliferation and maintaining endocrine cells survival in embryonic and postnatal pancreatic islets. However, how ISL1 exerts the role in adult islets is, to date, not clear.

Methodology/Principal Findings: Our results show that ISL1 expression was up-regulated at the mRNA level both in cultured pancreatic cells undergoing glucose oxidase stimulation as well in type 1 and type 2 diabetes mouse models. The knockdown of ISL1 expression increased the apoptosis level of HIT-T15 pancreatic islet cells. Using HIT-T15 and primary adult islet cells as cell models, we show that ISL1 promoted adult pancreatic islet cell proliferation with increased c-Myc and CyclinD1 transcription, while knockdown of ISL1 increased the proportion of cells in G(1) phase and decreased the proportion of cells in G(2)/M and S phases. Further investigation shows that ISL1 activated both c-Myc and CyclinD1 transcription through direct binding on their promoters.

Conclusions/Significance: ISL1 promoted adult pancreatic islet cell proliferation and probably by activating c-Myc and CyclinD1 transcription through direct binding on their promoters. Our findings extend the knowledge about the crucial role of ISL1 in maintaining mature islet cells homeostasis. Our results also provide insights into the new regulation relationships between ISL1 and other growth factors.

语种: 英语
所属项目编号: 30871253 ; 90919022 ; 81071675 ; B07001 ; 20060001107 ; 20070001798
项目资助者: National Natural Science Foundation of China ; 111 Project of China ; Specialized Research Fund for the Doctoral Program of Higher Education
WOS记录号: WOS:000293561200017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61549
Appears in Collections:基础医学院_期刊论文

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作者单位: Peking Univ, Minist Educ China, Sch Basic Med Sci, Dept Biochem & Mol Biol,Key Lab Mol Cardiovasc Sc, Beijing 100871, Peoples R China

Recommended Citation:
Guo, Ting,Wang, Weiping,Zhang, Hui,et al. ISL1 Promotes Pancreatic Islet Cell Proliferation[J]. PLOS ONE,2011,6(8).
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