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学科主题: 药学
题名:
Interactions between thrombin and natural products of Millettia nitita var. hirsutissima using capillary zone electrophoresis
作者: Zhang, Shuyu1; Cheng, Jun2; Chen, Wenjing1; Ling, Xiaomei1; Zhao, Yuying2; Feng, Jie2; Xiang, Cheng2; Liang, Hong2
关键词: Capillary zone electrophoresis ; Millettia nitita var. hirsutissima ; Interactions ; Thrombin
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2009-12-15
DOI: 10.1016/j.jchromb.2009.10.033
卷: 877, 期:32, 页:4107-4114
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: FLAVONOIDS
英文摘要:

A sensitive and selective high-performance analytical method based on capillary zone electrophoresis (CZE) was developed for investigating interactions between natural products isolated from Millettia nitita var. hirsutissima and thrombin qualitatively and quantitatively for the first time. The results showed that, compared with positive and negative control, the compounds ZYY-5 (genistein-8-C-beta-D-apiofuranosyl-(1 -> 6)-O-beta-D-glucopyranoside), ZYY-6 (calycosin). ZYY-8 (isoliquiritigenin), ZYY-9 (formononetin), ZYY-12 (gliricidin), ZYY-13 (8-O-methylretusin), FJ-2 (dihydrokaempferol). FJ-3 (biochanin), FJ-5 (afromosin) and XC-2 (hirsutissimiside F) interacted with thrombin, while ZYY-1 (sphaerobioside), ZYY-2 (formononetin-7-O-beta-D-apiofuranosyl-(1 -> 6)-O-beta-D-glucopyranoside), ZYY-3 (genistein-5-methylether-7-O-alpha-L-rhamnopyranosyl-(1 -> 6)-O-beta-D-glucopyranoside), ZYY-4 (retusin-7,8-O-beta-D-diglucopyranoside), ZYY-7 (symplocoside), ZYY-10 (ononin), ZYY-11 (genistin), ZYY-14 (afromosin-7-O-beta-D-glucopyranoside), ZYY-15(lanceolarin), FJ-1 (liquiritigenin), FJ-4 (7,2-dihydroxy, 4-methoxyisoflavan) and XC-1 (sphaerobioside) had no binding to thrombin. This indicated that the reported CZE method for the determination of compound-thrombin interactions is powerful, sensitive and fast. and requires less amounts of reagents, and further, it can be employed as a reliable alternative to other methods. (C) 2009 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 20432030 ; 985-2-063-112
项目资助者: National Nature Science Foundation of China ; Changjiang Scholar and Innovative Team in University
WOS记录号: WOS:000272881000005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61553
Appears in Collections:北京大学药学院_化学生物学系_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut Anal, Beijing 100191, Peoples R China
2.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China

Recommended Citation:
Zhang, Shuyu,Cheng, Jun,Chen, Wenjing,et al. Interactions between thrombin and natural products of Millettia nitita var. hirsutissima using capillary zone electrophoresis[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2009,877(32):4107-4114.
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