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学科主题: 口腔医学
题名:
ZO-1 and-2 Are Required for TRPVI-Modulated Paracellular Permeability
作者: Li, J.1; Cong, X.2,3; Zhang, Y.2,3; Xiang, R. L.2,3; Mei, M.1; Yang, N. Y.4; Su, Y. C.2,3; Choi, S.5; Park, K.5; Zhang, L. W.6; Wu, L. L.2,3; Yu, G. Y.1
关键词: zonula occludens ; tight junction ; capsaicin ; transient receptor potential vanilloid subtype 1 ; salivary cells ; salivation
刊名: JOURNAL OF DENTAL RESEARCH
发表日期: 2015-12-01
DOI: 10.1177/0022034515609268
卷: 94, 期:12, 页:1748-1756
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Dentistry, Oral Surgery & Medicine
研究领域[WOS]: Dentistry, Oral Surgery & Medicine
关键词[WOS]: TIGHT JUNCTION PROTEINS ; ZONULA OCCLUDENS-1 AND-2 ; SUBMANDIBULAR-GLAND ; SALIVARY-GLANDS ; BARRIER INTEGRITY ; EXPRESSION ; CELLS ; CYTOSKELETON ; RECEPTOR ; OCCLUDIN
英文摘要:

The tight junction based paracellular pathway plays an important role in saliva secretion. Zonula occludens (ZO) proteins are submembranous proteins of tight junction complex; however, their function in salivary epithelium is poorly understood. Here, we found that activation of transient receptor potential vanilloid subtype 1 (TRPV I) by capsaicin increased rat saliva secretion both in vivo and ex vivo. Meanwhile, TRPV I activation enlarged the width of tight junctions between neighboring acinar cells, increased the paracellular flux of 4-kDa fluorescein isothiocyanate (FITC)-dextran in submandibular gland (SMG) tissues, and decreased transepithelial electric resistance (TER) in SMG-C6 cells. ZO-1, -2, and -3 were distributed principally to the apical lateral region of acinar cells in SMG tissues and continuously encircled the peripheries of SMG-C6 cells in the untreated condition. TRPV I activation obviously diminished ZO-1 and -2 staining, but not ZO-3 or f3-catenin, at the cell-cell contacts ex vivo and in vitro. Moreover, in untreated SMG-C6 cells, ZO-1 and -2 single or double knockdown by small interfering RNA (siRNA) increased the paracellular flux of 4-kDa FITC-dextran. In capsaicintreated cells, ZO-1 and -2 single or double knockdown abolished, whereas their re-expression restored, the capsaicin-induced increase in paracellular permeability. Furthermore, TRPV I activation increased RhoA activity, and inhibition of either RhoA or Rho kinase (ROCK) abolished the capsaicin-induced TER decrease as well as ZO-1 and -2 redistribution. These results indicate that ZO-1 and -2 play crucial roles in both basal salivary epithelial barrier function and TRPV I -modulated paracellular transport. RhoA-ROCK signaling pathway is responsible for TRPVI -modulated paracellular permeability as well as ZO-1 and -2 redistribution.

语种: 英语
所属项目编号: 81271161 ; 81470756 ; 81311140269 ; 81170974 ; 20120001110045 ; 7132201
项目资助者: National Natural Science Foundation of China ; Ministry of Education of China ; Beijing Natural Science Foundation
WOS记录号: WOS:000365334400017
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61555
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Key Lab Mol Cardiovasc Sci,Minist Educ, Beijing 100191, Peoples R China
3.Beijing Key Lab Cardiovasc Receptors Res, Beijing, Peoples R China
4.Capital Med Univ, Beijing Stomatol Hosp, Dept Pediat Dent, Beijing, Peoples R China
5.Seoul Natl Univ, Sch Dent, Dept Physiol, Seoul, South Korea
6.Peking Univ, Sch & Hosp Stomatol, Dept Oral Med, Beijing 100191, Peoples R China

Recommended Citation:
Li, J.,Cong, X.,Zhang, Y.,et al. ZO-1 and-2 Are Required for TRPVI-Modulated Paracellular Permeability[J]. JOURNAL OF DENTAL RESEARCH,2015,94(12):1748-1756.
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