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学科主题: 临床医学
题名:
Gene Expression Profile Determination in Rat Retinal Tissue after Ocular Hypertension through Oligo Microarray
作者: Cui, Xin1; Xu, Ning3; Liao, Wenqiang4; Xu, Jiantao1; Ge, Chengguo2; Ye, Jian1; Liu, Xiangyuan3; He, Xiangge1
关键词: Glaucoma ; Ocular hypertension ; Oligomicroarray ; Gene expression profile
刊名: OPHTHALMIC RESEARCH
发表日期: 2011
DOI: 10.1159/000315619
卷: 45, 期:2, 页:57-64
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Ophthalmology
研究领域[WOS]: Ophthalmology
关键词[WOS]: VISUAL IMPAIRMENT ; ANGIOGENESIS ; PLEIOTROPHIN ; GLAUCOMA ; PCR
英文摘要:

Increased intraocular pressure is the main cause of glaucoma development. However, the systemic information of genes related to ocular hypertension has not yet been clarified. In the present study, oligomicroarray determined the profile of gene expression in the retina after ocular hypertension. A rat ocular hypertension model was constructed through photocoagulation by diode lasers. On postoperative days 7, 35, 60, 90, 180 and 360, the intraocular pressure and the gene expression profile were determined using an ophthalmotonometer and an Oligochip containing 35,000 oligonucleotides, respectively. Oligochip reliability was verified by real-time PCR, and the Oligochip data were analyzed through functional distribution analysis. In our study, we found that the intraocular pressure was significantly increased in a time-dependent manner but returned to the normal level on postoperative day 360. We also found that 1,692 genes were differentially expressed, including 719 upregulated and 973 downregulated genes. The chi(2) value of gene clusters related to transport function is significantly higher than that of other gene clusters as determined through function distribution analysis, suggesting that this group of genes plays an important role in the repair process of the optical nerve. In conclusion, the gene expression pattern at different time points of ocular hypertension was determined, which may contribute to clarify the molecular mechanism of glaucoma and to establish better therapeutic strategies to treat glaucoma. Copyright (C) 2010 S. Karger AG, Basel

语种: 英语
所属项目编号: 30801273
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000281237300001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61562
Appears in Collections:北京大学第三临床医学院_期刊论文

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作者单位: 1.Third Mil Med Univ, Daping Hosp, Dept Ophthalmol, Inst Surg Res, Chongqing 400042, Peoples R China
2.Third Mil Med Univ, Daping Hosp, Dept Urinary Surg, Inst Surg Res, Chongqing 400042, Peoples R China
3.Peking Univ, Hosp 3, Dept Rheumatol & Immunol, Beijing 100871, Peoples R China
4.China Japan Friendship Hosp, Natl Integrat Med Ctr Cardiovasc Dis, Beijing, Peoples R China

Recommended Citation:
Cui, Xin,Xu, Ning,Liao, Wenqiang,et al. Gene Expression Profile Determination in Rat Retinal Tissue after Ocular Hypertension through Oligo Microarray[J]. OPHTHALMIC RESEARCH,2011,45(2):57-64.
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