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学科主题临床医学
Gene Expression Profile Determination in Rat Retinal Tissue after Ocular Hypertension through Oligo Microarray
Cui, Xin1; Xu, Ning3; Liao, Wenqiang4; Xu, Jiantao1; Ge, Chengguo2; Ye, Jian1; Liu, Xiangyuan3; He, Xiangge1
关键词Glaucoma Ocular hypertension Oligomicroarray Gene expression profile
刊名OPHTHALMIC RESEARCH
2011
DOI10.1159/000315619
45期:2页:57-64
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Ophthalmology
研究领域[WOS]Ophthalmology
关键词[WOS]VISUAL IMPAIRMENT ; ANGIOGENESIS ; PLEIOTROPHIN ; GLAUCOMA ; PCR
英文摘要

Increased intraocular pressure is the main cause of glaucoma development. However, the systemic information of genes related to ocular hypertension has not yet been clarified. In the present study, oligomicroarray determined the profile of gene expression in the retina after ocular hypertension. A rat ocular hypertension model was constructed through photocoagulation by diode lasers. On postoperative days 7, 35, 60, 90, 180 and 360, the intraocular pressure and the gene expression profile were determined using an ophthalmotonometer and an Oligochip containing 35,000 oligonucleotides, respectively. Oligochip reliability was verified by real-time PCR, and the Oligochip data were analyzed through functional distribution analysis. In our study, we found that the intraocular pressure was significantly increased in a time-dependent manner but returned to the normal level on postoperative day 360. We also found that 1,692 genes were differentially expressed, including 719 upregulated and 973 downregulated genes. The chi(2) value of gene clusters related to transport function is significantly higher than that of other gene clusters as determined through function distribution analysis, suggesting that this group of genes plays an important role in the repair process of the optical nerve. In conclusion, the gene expression pattern at different time points of ocular hypertension was determined, which may contribute to clarify the molecular mechanism of glaucoma and to establish better therapeutic strategies to treat glaucoma. Copyright (C) 2010 S. Karger AG, Basel

语种英语
WOS记录号WOS:000281237300001
项目编号30801273
资助机构National Natural Science Foundation of China
引用统计
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61562
专题北京大学第三临床医学院
作者单位1.Third Mil Med Univ, Daping Hosp, Dept Ophthalmol, Inst Surg Res, Chongqing 400042, Peoples R China
2.Third Mil Med Univ, Daping Hosp, Dept Urinary Surg, Inst Surg Res, Chongqing 400042, Peoples R China
3.Peking Univ, Hosp 3, Dept Rheumatol & Immunol, Beijing 100871, Peoples R China
4.China Japan Friendship Hosp, Natl Integrat Med Ctr Cardiovasc Dis, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Cui, Xin,Xu, Ning,Liao, Wenqiang,et al. Gene Expression Profile Determination in Rat Retinal Tissue after Ocular Hypertension through Oligo Microarray[J]. OPHTHALMIC RESEARCH,2011,45(2):57-64.
APA Cui, Xin.,Xu, Ning.,Liao, Wenqiang.,Xu, Jiantao.,Ge, Chengguo.,...&He, Xiangge.(2011).Gene Expression Profile Determination in Rat Retinal Tissue after Ocular Hypertension through Oligo Microarray.OPHTHALMIC RESEARCH,45(2),57-64.
MLA Cui, Xin,et al."Gene Expression Profile Determination in Rat Retinal Tissue after Ocular Hypertension through Oligo Microarray".OPHTHALMIC RESEARCH 45.2(2011):57-64.
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