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学科主题: 临床医学
题名:
Characteristics of mitotic cell death induced by enediyne antibiotic lidamycin in human epithelial tumor cells
作者: He, QY; Liang, YY; Wang, DS; Li, DD
关键词: mitotic cell death ; apoptosis ; cellular senescence ; lidamycin ; hepatoma BEL-7402 cells ; breast carcinoma MCF-7 cells
刊名: INTERNATIONAL JOURNAL OF ONCOLOGY
发表日期: 2002-02-01
卷: 20, 期:2, 页:261-266
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: TERMINAL PROLIFERATION ARREST ; HELA-CELLS ; DNA-DAMAGE ; APOPTOSIS ; SENESCENCE ; C-1027 ; DRUG ; RADIATION ; CANCER ; C1027
英文摘要:

Mitotic cell death, a different cell death mode from apoptosis, has been focused on in tumor therapy. It may involve the mechanism of highly potent cytotoxicities of enediyne antibiotics toward tumor cells. We describe the characteristics of mitotic cell death induced by enediyne antibiotic lidamycin at low concentrations (0.01-1 nM), in the human hepatoma BEL-7402 cells and human breast carcinoma MCF-7 cells. The cells exerting mitotic cell death showed retardation at G(2)+M phase, enlargement of cell volume and multi nucleation, some of which were positive in senescence-associate beta-galactosidase staining. The multinucleated living cells did not show apoptotic features by co-staining with mitochondria-specific dye Mitosensor and DNA-specific dye Hoechst 33342. The DNA polyploidy rather than ′apoptotic sub-G(1) peak′ increased with incubation time for the lidamycin-treated BEL-7402 cells. The proliferation status of BEL-7402 cells was shown by flow cytometry after the cells were labeled with PKH-67, a fluorescent dye for labeling living cells, but the fluorescent intensity of the lidamycin-treated cells was little changed. The smear DNA pattern was detected in the multinucleated cells by agarose gel electrophoresis. The results provide the first evidence for elucidating the potent cytotoxicities of lidamycin toward tumor cells and further describing characteristics of mitotic cell death.

语种: 英语
WOS记录号: WOS:000173462900005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61563
Appears in Collections:北京大学临床肿瘤学院_期刊论文

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作者单位: 1.Chinese Acad Med Sci, Inst Med Biotechnol, Beijing 100050, Peoples R China
2.Beijing Union Med Coll, Beijing, Peoples R China
3.Peking Univ, Sch Oncol, Beijing Inst Canc Res, Beijing 100871, Peoples R China

Recommended Citation:
He, QY,Liang, YY,Wang, DS,et al. Characteristics of mitotic cell death induced by enediyne antibiotic lidamycin in human epithelial tumor cells[J]. INTERNATIONAL JOURNAL OF ONCOLOGY,2002,20(2):261-266.
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