IR@PKUHSC  > 北京大学临床肿瘤学院
学科主题临床医学
Characteristics of mitotic cell death induced by enediyne antibiotic lidamycin in human epithelial tumor cells
He, QY; Liang, YY; Wang, DS; Li, DD
关键词mitotic cell death apoptosis cellular senescence lidamycin hepatoma BEL-7402 cells breast carcinoma MCF-7 cells
刊名INTERNATIONAL JOURNAL OF ONCOLOGY
2002-02-01
20期:2页:261-266
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]TERMINAL PROLIFERATION ARREST ; HELA-CELLS ; DNA-DAMAGE ; APOPTOSIS ; SENESCENCE ; C-1027 ; DRUG ; RADIATION ; CANCER ; C1027
英文摘要

Mitotic cell death, a different cell death mode from apoptosis, has been focused on in tumor therapy. It may involve the mechanism of highly potent cytotoxicities of enediyne antibiotics toward tumor cells. We describe the characteristics of mitotic cell death induced by enediyne antibiotic lidamycin at low concentrations (0.01-1 nM), in the human hepatoma BEL-7402 cells and human breast carcinoma MCF-7 cells. The cells exerting mitotic cell death showed retardation at G(2)+M phase, enlargement of cell volume and multi nucleation, some of which were positive in senescence-associate beta-galactosidase staining. The multinucleated living cells did not show apoptotic features by co-staining with mitochondria-specific dye Mitosensor and DNA-specific dye Hoechst 33342. The DNA polyploidy rather than ′apoptotic sub-G(1) peak′ increased with incubation time for the lidamycin-treated BEL-7402 cells. The proliferation status of BEL-7402 cells was shown by flow cytometry after the cells were labeled with PKH-67, a fluorescent dye for labeling living cells, but the fluorescent intensity of the lidamycin-treated cells was little changed. The smear DNA pattern was detected in the multinucleated cells by agarose gel electrophoresis. The results provide the first evidence for elucidating the potent cytotoxicities of lidamycin toward tumor cells and further describing characteristics of mitotic cell death.

语种英语
WOS记录号WOS:000173462900005
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被引频次:31[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61563
专题北京大学临床肿瘤学院
作者单位1.Chinese Acad Med Sci, Inst Med Biotechnol, Beijing 100050, Peoples R China
2.Beijing Union Med Coll, Beijing, Peoples R China
3.Peking Univ, Sch Oncol, Beijing Inst Canc Res, Beijing 100871, Peoples R China
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He, QY,Liang, YY,Wang, DS,et al. Characteristics of mitotic cell death induced by enediyne antibiotic lidamycin in human epithelial tumor cells[J]. INTERNATIONAL JOURNAL OF ONCOLOGY,2002,20(2):261-266.
APA He, QY,Liang, YY,Wang, DS,&Li, DD.(2002).Characteristics of mitotic cell death induced by enediyne antibiotic lidamycin in human epithelial tumor cells.INTERNATIONAL JOURNAL OF ONCOLOGY,20(2),261-266.
MLA He, QY,et al."Characteristics of mitotic cell death induced by enediyne antibiotic lidamycin in human epithelial tumor cells".INTERNATIONAL JOURNAL OF ONCOLOGY 20.2(2002):261-266.
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