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A high-sensitivity LC-MS/MS method for the determination of 4-methyl-piperazine-1-carbodithioc acid 3-cyano-3, 3-diphenylpropyl ester hydrochloride in rat plasma and its application to a pharmacokinetics study
Ji, Xiwei; Chen, Ye; Li, Runtao; Zhou, Tianyan; Lu, Wei
关键词LC-MS MS TM208 plasma concentration pharmacokinetics
刊名BIOMEDICAL CHROMATOGRAPHY
2012-10-01
DOI10.1002/bmc.2678
26期:10页:1196-1201
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology ; Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry ; Pharmacology & Pharmacy
关键词[WOS]TANDEM MASS-SPECTROMETRY ; DITHIOCARBAMATE ; METABOLITES ; DERIVATIVES ; TM208 ; HPLC
英文摘要

4-Methyl-piperazine-1-carbodithioc acid 3-cyano-3, 3-diphenylpropyl ester hydrochloride (TM208), a newly synthesized anticancer compound, was quantified using liquid chromatographytandem mass spectrometry (LC-MS/MS) for the first time. A simple, rapid and sensitive assay method using propranolol as internal standard (IS) after one-step precipitation with acetonitrile was developed and validated to determine TM208 in rat plasma. Separation was achieved on a reverse-phase C18 column with a mobile phase composed of methanolwater (pH4.0) containing 5?m m ammonium acetate in gradient elution mode. A triple quadrupole tandem mass spectrometer with electrospray ionization source was used as detector and operated by multiple reaction monitoring in the positive ion mode. Calibration curves were linear (r?>?0.99) between 0.2 and 500?ng/mL. The quantitative limit was 0.2?ng/mL; reliable precision and accuracy were validated by relative standard deviation values in the range 3.4413.15% and relative error values between -0.58 and -9.78%. The method was successfully applied to preclinical pharmacokinetic studies of TM208. Copyright (c) 2011 John Wiley & Sons, Ltd.

语种英语
WOS记录号WOS:000308289200011
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61571
专题北京大学药学院
北京大学药学院_药剂学系
作者单位Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Hlth Sci Ctr, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Ji, Xiwei,Chen, Ye,Li, Runtao,et al. A high-sensitivity LC-MS/MS method for the determination of 4-methyl-piperazine-1-carbodithioc acid 3-cyano-3, 3-diphenylpropyl ester hydrochloride in rat plasma and its application to a pharmacokinetics study[J]. BIOMEDICAL CHROMATOGRAPHY,2012,26(10):1196-1201.
APA Ji, Xiwei,Chen, Ye,Li, Runtao,Zhou, Tianyan,&Lu, Wei.(2012).A high-sensitivity LC-MS/MS method for the determination of 4-methyl-piperazine-1-carbodithioc acid 3-cyano-3, 3-diphenylpropyl ester hydrochloride in rat plasma and its application to a pharmacokinetics study.BIOMEDICAL CHROMATOGRAPHY,26(10),1196-1201.
MLA Ji, Xiwei,et al."A high-sensitivity LC-MS/MS method for the determination of 4-methyl-piperazine-1-carbodithioc acid 3-cyano-3, 3-diphenylpropyl ester hydrochloride in rat plasma and its application to a pharmacokinetics study".BIOMEDICAL CHROMATOGRAPHY 26.10(2012):1196-1201.
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