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A Novel and Facile Approach to Imaging Nanoparticles Transport across Transwell Filter Grown Cell Mono layer in Real-Time and in Situ under Confocal Laser Scanning Microscopy
Zhao, Shanshan2; Yuan, Lan1; Wang, Jiancheng; Zhang, Xuan; He, Zhonggui2; Zhang, Qiang2
关键词methodology cellular transport confocal laser scanning microscopy Transwell real-time in situ
刊名BIOLOGICAL & PHARMACEUTICAL BULLETIN
2012-03-01
35期:3页:335-345
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Pharmacology & Pharmacy
资助者National Basic Research Program of China ; State Key Projects ; 863 Project ; National Basic Research Program of China ; State Key Projects ; 863 Project
研究领域[WOS]Pharmacology & Pharmacy
关键词[WOS]POLARIZED EPITHELIAL-CELLS ; MICELLES ; DELIVERY ; ENDOCYTOSIS ; ABSORPTION ; MECHANISMS ; CHLORIDE ; DRUGS ; TUMOR ; MODEL
英文摘要

Although the cellular endocytosis or uptake research using confocal laser scanning microscopy (CLSM) and Transwell inserts was frequently reported in experimental cell research and pharmaceutical research, there is little report on cellular transport process based on the same techniques. One of main reasons is that fluorescence of most fluorescent reagents could be clearly and definitely seen under CLSM only when they are internalized into and concentrated within cells. Here, Madin-Darby Canine Kidney (MDCK) cells and Coumarin 6 labeled nanoparticles (C6-NPs) was used as models, a new system was developed to image C6-NPs transport across Transwell filter grown cell monolayer by adding free cells into the basolateral medium and making the Transwell insert semi-permeable membrane visible under CLSM. The transport process could be clearly imaged in real-time and in situ, based on the visualization of Transwell membrane indicating the relative position of cells and nanoparticles, and the free cells in the basolateral medium serving as collector and indicator for the transported nanoparticles. The method was also applied in cell migration study. We believe that the novel approach developed here will be certainly useful not only in exploration of nanoparticle cellular transport mechanism, but also in other cell biological sciences based on CLSM and Transwell.

语种英语
所属项目编号2009CB930300 ; 2009ZX09310-001 ; 2007AA021811
资助者National Basic Research Program of China ; State Key Projects ; 863 Project ; National Basic Research Program of China ; State Key Projects ; 863 Project
WOS记录号WOS:000300925200009
引用统计
被引频次:5[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61576
专题北京大学药学院
作者单位1.Peking Univ, Sch Pharmaceut Sci, Med & Healthy Anal Ctr, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Shenyang Pharmaceut Univ, Dept Pharmaceut, Sch Pharm, Shenyang 110016, Peoples R China
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GB/T 7714
Zhao, Shanshan,Yuan, Lan,Wang, Jiancheng,et al. A Novel and Facile Approach to Imaging Nanoparticles Transport across Transwell Filter Grown Cell Mono layer in Real-Time and in Situ under Confocal Laser Scanning Microscopy[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2012,35(3):335-345.
APA Zhao, Shanshan,Yuan, Lan,Wang, Jiancheng,Zhang, Xuan,He, Zhonggui,&Zhang, Qiang.(2012).A Novel and Facile Approach to Imaging Nanoparticles Transport across Transwell Filter Grown Cell Mono layer in Real-Time and in Situ under Confocal Laser Scanning Microscopy.BIOLOGICAL & PHARMACEUTICAL BULLETIN,35(3),335-345.
MLA Zhao, Shanshan,et al."A Novel and Facile Approach to Imaging Nanoparticles Transport across Transwell Filter Grown Cell Mono layer in Real-Time and in Situ under Confocal Laser Scanning Microscopy".BIOLOGICAL & PHARMACEUTICAL BULLETIN 35.3(2012):335-345.
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