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学科主题: 药学
题名:
A Novel and Facile Approach to Imaging Nanoparticles Transport across Transwell Filter Grown Cell Mono layer in Real-Time and in Situ under Confocal Laser Scanning Microscopy
作者: Zhao, Shanshan2; Yuan, Lan1; Wang, Jiancheng; Zhang, Xuan; He, Zhonggui2; Zhang, Qiang2
关键词: methodology ; cellular transport ; confocal laser scanning microscopy ; Transwell ; real-time ; in situ
刊名: BIOLOGICAL & PHARMACEUTICAL BULLETIN
发表日期: 2012-03-01
卷: 35, 期:3, 页:335-345
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Pharmacology & Pharmacy
研究领域[WOS]: Pharmacology & Pharmacy
关键词[WOS]: POLARIZED EPITHELIAL-CELLS ; MICELLES ; DELIVERY ; ENDOCYTOSIS ; ABSORPTION ; MECHANISMS ; CHLORIDE ; DRUGS ; TUMOR ; MODEL
英文摘要:

Although the cellular endocytosis or uptake research using confocal laser scanning microscopy (CLSM) and Transwell inserts was frequently reported in experimental cell research and pharmaceutical research, there is little report on cellular transport process based on the same techniques. One of main reasons is that fluorescence of most fluorescent reagents could be clearly and definitely seen under CLSM only when they are internalized into and concentrated within cells. Here, Madin-Darby Canine Kidney (MDCK) cells and Coumarin 6 labeled nanoparticles (C6-NPs) was used as models, a new system was developed to image C6-NPs transport across Transwell filter grown cell monolayer by adding free cells into the basolateral medium and making the Transwell insert semi-permeable membrane visible under CLSM. The transport process could be clearly imaged in real-time and in situ, based on the visualization of Transwell membrane indicating the relative position of cells and nanoparticles, and the free cells in the basolateral medium serving as collector and indicator for the transported nanoparticles. The method was also applied in cell migration study. We believe that the novel approach developed here will be certainly useful not only in exploration of nanoparticle cellular transport mechanism, but also in other cell biological sciences based on CLSM and Transwell.

语种: 英语
所属项目编号: 2009CB930300 ; 2009ZX09310-001 ; 2007AA021811
项目资助者: National Basic Research Program of China ; State Key Projects ; 863 Project
WOS记录号: WOS:000300925200009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61576
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Med & Healthy Anal Ctr, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Shenyang Pharmaceut Univ, Dept Pharmaceut, Sch Pharm, Shenyang 110016, Peoples R China

Recommended Citation:
Zhao, Shanshan,Yuan, Lan,Wang, Jiancheng,et al. A Novel and Facile Approach to Imaging Nanoparticles Transport across Transwell Filter Grown Cell Mono layer in Real-Time and in Situ under Confocal Laser Scanning Microscopy[J]. BIOLOGICAL & PHARMACEUTICAL BULLETIN,2012,35(3):335-345.
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