|Profiling of microRNA-mRNA reveals roles of microRNAs in cervical cancer|
|Ma Ding1; Zhang You-yi2,3; Guo Yan-li1; Li Zi-jian2,3; Geng Li1|
|关键词||cervical cancer microRNA gene expression microarray|
|刊名||CHINESE MEDICAL JOURNAL|
|WOS标题词||Science & Technology|
|类目[WOS]||Medicine, General & Internal|
|研究领域[WOS]||General & Internal Medicine|
|关键词[WOS]||EXPRESSION ; GENES ; TARGET ; CELLS ; MODEL ; IDENTIFICATION ; CARCINOMA ; PATHWAY ; GENOME ; GROWTH|
Background Cervical cancer is one of the most common malignant tumors in women. This study was designed to explore the expression profiles of microRNAs (miRNAs) and mRNAs and the gene regulation network in cervical tumorigenesis and to find candidate molecular markers and key tumorigenic genes in cervical cancer.
Methods miRNAs and mRNAs expression microarrays were used to detect the expression of miRNAs and mRNAs in normal and cancer cervical tissues. Target Scan 5.0 database (UK) was used to predict the target genes of the miRNAs, analyze their intersection with differentially expressed mRNAs and negatively correlate the intersection with miRNAs. Bioinformatic approaches were used to analyze functions and pathways of the target genes and establish miRNA-gene network.
Results Twenty-nine miRNAs and 2036 mRNAs were differentially expressed in normal and cervical tumor tissues. Among them, 13 miRNAs and 754 mRNAs were up-regulated in cervical tumor tissues and 16 miRNAs and 1282 RNA were down-regulated. The 327 target genes negatively related to miRNAs in the intersection were involved in functions and signal pathways. Down-regulated miRNAs targeted genes and up-regulated miRNAs targeted genes were involved in 415 and 163 functions, respectively, and in 37 and 17 significant pathways, respectively (P <0.05, false discovery rate (FDR) <0.05). We constructed the miRNAs-gene network and found that hsa-miR-15a, hsa-miR-106b and hsa-miR-20b were key nodes in the network.
Conclusions The differentially expressed miRNAs and mRNAs in cervical cancer and related miRNA-gene network have been identified. They play important roles in cervical tumorigenesis and are involved in many important biological functions and signal transduction pathways. These findings lay a foundation for research on the molecular mechanism of miRNAs in the pathogenesis of cervical cancer. Chin Med J 2012;125(23):4270-4276
|项目编号||11072006 ; 81030001 ; 81070078|
|资助机构||National Natural Science Foundation of China|
|作者单位||1.Peking Univ Third Hosp, Dept Gynecol & Obstet, Beijing 100191, Peoples R China|
2.Peking Univ Third Hosp, Inst Vasc Med, Beijing 100191, Peoples R China
3.Minist Hlth, Key Lab Cardiovasc Mol Biol & Regulatory Peptide, Beijing 100191, Peoples R China
|Ma Ding,Zhang You-yi,Guo Yan-li,et al. Profiling of microRNA-mRNA reveals roles of microRNAs in cervical cancer[J]. CHINESE MEDICAL JOURNAL,2012,125(23):4270-4276.|
|APA||Ma Ding,Zhang You-yi,Guo Yan-li,Li Zi-jian,&Geng Li.(2012).Profiling of microRNA-mRNA reveals roles of microRNAs in cervical cancer.CHINESE MEDICAL JOURNAL,125(23),4270-4276.|
|MLA||Ma Ding,et al."Profiling of microRNA-mRNA reveals roles of microRNAs in cervical cancer".CHINESE MEDICAL JOURNAL 125.23(2012):4270-4276.|