IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科
学科主题临床医学
Profiling of microRNA-mRNA reveals roles of microRNAs in cervical cancer
Ma Ding1; Zhang You-yi2,3; Guo Yan-li1; Li Zi-jian2,3; Geng Li1
关键词cervical cancer microRNA gene expression microarray
刊名CHINESE MEDICAL JOURNAL
2012-12-05
DOI10.3760/cma.j.issn.0366-6999.2012.23.020
125期:23页:4270-4276
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]EXPRESSION ; GENES ; TARGET ; CELLS ; MODEL ; IDENTIFICATION ; CARCINOMA ; PATHWAY ; GENOME ; GROWTH
英文摘要

Background Cervical cancer is one of the most common malignant tumors in women. This study was designed to explore the expression profiles of microRNAs (miRNAs) and mRNAs and the gene regulation network in cervical tumorigenesis and to find candidate molecular markers and key tumorigenic genes in cervical cancer.

Methods miRNAs and mRNAs expression microarrays were used to detect the expression of miRNAs and mRNAs in normal and cancer cervical tissues. Target Scan 5.0 database (UK) was used to predict the target genes of the miRNAs, analyze their intersection with differentially expressed mRNAs and negatively correlate the intersection with miRNAs. Bioinformatic approaches were used to analyze functions and pathways of the target genes and establish miRNA-gene network.

Results Twenty-nine miRNAs and 2036 mRNAs were differentially expressed in normal and cervical tumor tissues. Among them, 13 miRNAs and 754 mRNAs were up-regulated in cervical tumor tissues and 16 miRNAs and 1282 RNA were down-regulated. The 327 target genes negatively related to miRNAs in the intersection were involved in functions and signal pathways. Down-regulated miRNAs targeted genes and up-regulated miRNAs targeted genes were involved in 415 and 163 functions, respectively, and in 37 and 17 significant pathways, respectively (P <0.05, false discovery rate (FDR) <0.05). We constructed the miRNAs-gene network and found that hsa-miR-15a, hsa-miR-106b and hsa-miR-20b were key nodes in the network.

Conclusions The differentially expressed miRNAs and mRNAs in cervical cancer and related miRNA-gene network have been identified. They play important roles in cervical tumorigenesis and are involved in many important biological functions and signal transduction pathways. These findings lay a foundation for research on the molecular mechanism of miRNAs in the pathogenesis of cervical cancer. Chin Med J 2012;125(23):4270-4276

语种英语
WOS记录号WOS:000312748200020
引用统计
被引频次:25[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61585
专题北京大学第三临床医学院_心血管内科
北京大学第三临床医学院_妇产科
作者单位1.Peking Univ Third Hosp, Dept Gynecol & Obstet, Beijing 100191, Peoples R China
2.Peking Univ Third Hosp, Inst Vasc Med, Beijing 100191, Peoples R China
3.Minist Hlth, Key Lab Cardiovasc Mol Biol & Regulatory Peptide, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Ma Ding,Zhang You-yi,Guo Yan-li,et al. Profiling of microRNA-mRNA reveals roles of microRNAs in cervical cancer[J]. CHINESE MEDICAL JOURNAL,2012,125(23):4270-4276.
APA Ma Ding,Zhang You-yi,Guo Yan-li,Li Zi-jian,&Geng Li.(2012).Profiling of microRNA-mRNA reveals roles of microRNAs in cervical cancer.CHINESE MEDICAL JOURNAL,125(23),4270-4276.
MLA Ma Ding,et al."Profiling of microRNA-mRNA reveals roles of microRNAs in cervical cancer".CHINESE MEDICAL JOURNAL 125.23(2012):4270-4276.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Ma Ding]的文章
[Zhang You-yi]的文章
[Guo Yan-li]的文章
百度学术
百度学术中相似的文章
[Ma Ding]的文章
[Zhang You-yi]的文章
[Guo Yan-li]的文章
必应学术
必应学术中相似的文章
[Ma Ding]的文章
[Zhang You-yi]的文章
[Guo Yan-li]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。