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A rapid and sensitive HPLC-MS/MS analysis and preliminary pharmacokinetic characterization of sibiricaxanthone F in rats
Yang, Xiaojuan; Zhou, Guanshen; Zou, Pingping; Ning, Ying; Zan, Ke; Tu, Pengfei; Jiang, Yong
关键词Sibiricaxanthone F Xanthone Pharmacokinetic Bioavailability LC-MS/MS Polygala
刊名JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
2011-09-01
DOI10.1016/j.jchromb.2011.07.002
879期:25页:2513-2518
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]CHROMATOGRAPHY-MASS SPECTROMETRY ; POLYGALA-TENUIFOLIA ; XANTHONE GLYCOSIDES ; PLASMA ; ROOTS ; EXTRACT ; BT-11
英文摘要

A simple, rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for quantifying sibiricaxanthone F (SF) in rat plasma following oral and intravenous dosings. After addition of the internal standard (IS) kaempferol and the antioxidant, 20% ascorbic acid, plasma samples were precipitated with acetonitrile and separated on an Aglient Zorbax XDB-C(18) column (50 mm x 4.6 mm ID., 2.1 mu m) with gradient acetonitrile and water (both containing 0.01% formic acid) as the mobile phase. The detection was performed on a Sciex API 4000 LC-MS/MS with electrospray ionization (ESI) inlet in the negative multiple reaction monitoring (MRM) mode. Good linearity was achieved over the concentration range of 0.5-500.0 ng/mL (r > 0.996). Intra- and inter-day precisions were less than 7.60%, and accuracy ranged from 97.18% to 99.84%. The lower limit of quantification for SF was 0.5 ng/mL, and analytes were stable under various conditions (during freeze-thaw, at room temperature and under deep-freeze conditions). This validated method was successfully applied to the preliminary pharmacokinetic study of SF in rats for the first time, and the absolute bioavailability of SF was found to be only 0.22 +/- 0.15%. (C) 2011 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000294982600006
项目编号985-2-102-113 ; 30600778 ; 2009ZX09311-004
资助机构Ministry of Education of China ; National Natural Science Foundation of China ; National Key Technology R&amp ; D Program "New Drug Innovation" of China
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61593
专题北京大学药学院
北京大学药学院_天然药物学系
通讯作者Jiang, Yong
作者单位Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Yang, Xiaojuan,Zhou, Guanshen,Zou, Pingping,et al. A rapid and sensitive HPLC-MS/MS analysis and preliminary pharmacokinetic characterization of sibiricaxanthone F in rats[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(25):2513-2518.
APA Yang, Xiaojuan.,Zhou, Guanshen.,Zou, Pingping.,Ning, Ying.,Zan, Ke.,...&Jiang, Yong.(2011).A rapid and sensitive HPLC-MS/MS analysis and preliminary pharmacokinetic characterization of sibiricaxanthone F in rats.JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,879(25),2513-2518.
MLA Yang, Xiaojuan,et al."A rapid and sensitive HPLC-MS/MS analysis and preliminary pharmacokinetic characterization of sibiricaxanthone F in rats".JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES 879.25(2011):2513-2518.
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