北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学药学院  > 期刊论文
学科主题: 药学
题名:
A rapid and sensitive HPLC-MS/MS analysis and preliminary pharmacokinetic characterization of sibiricaxanthone F in rats
作者: Yang, Xiaojuan; Zhou, Guanshen; Zou, Pingping; Ning, Ying; Zan, Ke; Tu, Pengfei; Jiang, Yong
关键词: Sibiricaxanthone F ; Xanthone ; Pharmacokinetic ; Bioavailability ; LC-MS/MS ; Polygala
刊名: JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
发表日期: 2011-09-01
DOI: 10.1016/j.jchromb.2011.07.002
卷: 879, 期:25, 页:2513-2518
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: CHROMATOGRAPHY-MASS SPECTROMETRY ; POLYGALA-TENUIFOLIA ; XANTHONE GLYCOSIDES ; PLASMA ; ROOTS ; EXTRACT ; BT-11
英文摘要:

A simple, rapid and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and validated for quantifying sibiricaxanthone F (SF) in rat plasma following oral and intravenous dosings. After addition of the internal standard (IS) kaempferol and the antioxidant, 20% ascorbic acid, plasma samples were precipitated with acetonitrile and separated on an Aglient Zorbax XDB-C(18) column (50 mm x 4.6 mm ID., 2.1 mu m) with gradient acetonitrile and water (both containing 0.01% formic acid) as the mobile phase. The detection was performed on a Sciex API 4000 LC-MS/MS with electrospray ionization (ESI) inlet in the negative multiple reaction monitoring (MRM) mode. Good linearity was achieved over the concentration range of 0.5-500.0 ng/mL (r > 0.996). Intra- and inter-day precisions were less than 7.60%, and accuracy ranged from 97.18% to 99.84%. The lower limit of quantification for SF was 0.5 ng/mL, and analytes were stable under various conditions (during freeze-thaw, at room temperature and under deep-freeze conditions). This validated method was successfully applied to the preliminary pharmacokinetic study of SF in rats for the first time, and the absolute bioavailability of SF was found to be only 0.22 +/- 0.15%. (C) 2011 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 985-2-102-113 ; 30600778 ; 2009ZX09311-004
项目资助者: Ministry of Education of China ; National Natural Science Foundation of China ; National Key Technology R&amp ; D Program "New Drug Innovation" of China
WOS记录号: WOS:000294982600006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61593
Appears in Collections:北京大学药学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, State Key Lab Nat & Biomimet Drugs, Sch Pharmaceut Sci, Beijing 100191, Peoples R China

Recommended Citation:
Yang, Xiaojuan,Zhou, Guanshen,Zou, Pingping,et al. A rapid and sensitive HPLC-MS/MS analysis and preliminary pharmacokinetic characterization of sibiricaxanthone F in rats[J]. JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES,2011,879(25):2513-2518.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Yang, Xiaojuan]'s Articles
[Zhou, Guanshen]'s Articles
[Zou, Pingping]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Yang, Xiaojuan]‘s Articles
[Zhou, Guanshen]‘s Articles
[Zou, Pingping]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace