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Direct chemiluminescent imaging detection of human serum proteins in two-dimensional polyacrylamide gel electrophoresis
Tao, Oian; Wang, Zhenzhen; Zhao, Heping; Baeyens, Willy R. G.; Delanghe, Joris R.; Huang, Lingyun; Ouyang, Jin; He, Dacheng; Zhang, Xianhua
关键词chemiluminescent imaging cirrhotic patient haptoglobin human serum proteins two-dimensional gel electrophoresis
刊名PROTEOMICS
2007-10-01
DOI10.1002/pmic.200700078
7期:19页:3481-3490
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]HUMAN-PLASMA-PROTEINS ; HEMOPEXIN ; RESOLUTION ; LUMINOL ; HEME ; MAP
英文摘要

A novel chemiluminescence (CL)-based imaging method capable of directly detecting proteins in polyacrylamide gels after electrophoresis is proposed. Human serum proteins are presently detected by a direct CL imaging method after native 2-D PAGE. As a consequence, some proteins, including haptoglobin (Hp), Hp precursor, hemopexin (Hpx) precursor, Ig alpha-1 chain C region, and Complement C3 precursor can be detected and identified by MS and MS/MS techniques. These proteins are all acute phase proteins, which have been defined as biomarkers for certain diseases. Moreover, serum proteins from healthy people and cirrhotic patients were analyzed. A decrease in Hp spots for cirrhotic patients could be confirmed. The CL imaging conditions were optimized, including the concentrations of H2O2 and luminol. The process of CL detection of proteins is simple, and there is no need for specialized equipment. in comparison with the traditional CBB-R250 staining method, the detection sensitivity was improved and the detection period decreased about 70 times. Hence, this technique possesses potentials as a rapid, convenient, and inexpensive analytical technique for protein detection and for the diagnosis of diseases.

语种英语
WOS记录号WOS:000250283500006
引用统计
被引频次:7[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61598
专题北京大学第三临床医学院_药剂科
作者单位1.Univ Hosp, Dept Clin Chem Microbiol & Immunol, Ghent, Belgium
2.Peking Univ, Hosp 3, Dept Pharm, Beijing, Peoples R China
3.Beijing Normal Univ, Coll Chem, Beijing 100875, Peoples R China
4.Beijing Normal Univ, Inst Cell Biol, Beijing 100875, Peoples R China
5.Univ Ghent, Fac Pharmaceut Sci, Dept Pharmaceut Anal, B-9000 Ghent, Belgium
推荐引用方式
GB/T 7714
Tao, Oian,Wang, Zhenzhen,Zhao, Heping,et al. Direct chemiluminescent imaging detection of human serum proteins in two-dimensional polyacrylamide gel electrophoresis[J]. PROTEOMICS,2007,7(19):3481-3490.
APA Tao, Oian.,Wang, Zhenzhen.,Zhao, Heping.,Baeyens, Willy R. G..,Delanghe, Joris R..,...&Zhang, Xianhua.(2007).Direct chemiluminescent imaging detection of human serum proteins in two-dimensional polyacrylamide gel electrophoresis.PROTEOMICS,7(19),3481-3490.
MLA Tao, Oian,et al."Direct chemiluminescent imaging detection of human serum proteins in two-dimensional polyacrylamide gel electrophoresis".PROTEOMICS 7.19(2007):3481-3490.
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