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学科主题: 临床医学
题名:
TGF-beta insensitive dendritic cells: an efficient vaccine for murine prostate cancer
作者: Wang, Fu-Li; Qin, Wei-Jun; Wen, Wei-Hong; Tian, Feng; Song, Bin; Zhang, Qiang; Lee, Chung; Zhong, Wei-de; Guo, Ying-Lu; Wang, He
关键词: dendritic cell ; immuno-gene therapy ; prostate cancer ; receptor ; TGF-beta ; vaccination
刊名: CANCER IMMUNOLOGY IMMUNOTHERAPY
发表日期: 2007-11-01
DOI: 10.1007/s00262-007-0322-3
卷: 56, 期:11, 页:1785-1793
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Immunology
研究领域[WOS]: Oncology ; Immunology
关键词[WOS]: GROWTH-FACTOR-BETA ; TRANSFORMING GROWTH-FACTOR-BETA-1 ; FUSION VACCINE ; CLINICAL-TRIAL ; TUMOR-CELLS ; T-CELLS ; CARCINOMA ; MATURATION ; IMMUNITY ; ANGIOGENESIS
英文摘要:

Dendritic cells (DCs) are highly potent initiators of the immune response, but DC effector functions are often inhibited by immunosuppressants such as transforming growth factor beta (TGF-beta). The present study was conducted to develop a treatment strategy for prostate cancer using a TGF-beta-insensitive DC vaccine. Tumor lysate-pulsed DCs were rendered TGF-beta insensitive by dominant-negative TGF-beta type II receptor (T beta RIIDN), leading to the blockade of TGF-beta signals to members of the Smad family, which are the principal cytoplasmic intermediates involved in the transduction of signals from TGF-beta receptors to the nucleus. Expression of T beta RIIDN did not affect the phenotype of transduced DCs. Phosphorylated Smad-2 was undetectable and expression of surface co-stimulatory molecules (CD80/CD86) were upregulated in T beta RIIDN DCs after antigen and TGF-beta 1 stimulation. Vaccination of C57BL/6 tumor-bearing mice with the T beta RIIDN DC vaccine induced potent tumor-specific cytotoxic T lymphocyte responses against TRAMP-C2 tumors, increased serum IFN-gamma and IL-12 level, inhibited tumor growth and increased mouse survival. Furthermore, complete tumor regression occurred in two vaccinated mice. These results demonstrate that blocking TGF-beta signals in DC enhances the efficacy of DC-based vaccines.

语种: 英语
WOS记录号: WOS:000249017400010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61602
Appears in Collections:北京大学第一临床医学院_泌尿外科_期刊论文

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作者单位: 1.Peoples Hosp Guangzhou 1, Dept Urol, Guangzhou 510180, Peoples R China
2.Fourth Mil Med Univ, Xijing Hosp, Dept Urol, Xian 710032, Shaanxi, Peoples R China
3.Peking Univ, Peking Univ Hosp 1, Dept Urol, Inst Urol, Beijing 100043, Peoples R China
4.Fourth Mil Med Univ, Dept Immunol, Xian 710032, Peoples R China
5.Northwestern Univ, Feinberg Sch Med, Dept Urol, Chicago, IL 60611 USA

Recommended Citation:
Wang, Fu-Li,Qin, Wei-Jun,Wen, Wei-Hong,et al. TGF-beta insensitive dendritic cells: an efficient vaccine for murine prostate cancer[J]. CANCER IMMUNOLOGY IMMUNOTHERAPY,2007,56(11):1785-1793.
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