北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学临床肿瘤学院  > 期刊论文
学科主题: 临床医学
题名:
A Novel Retinoblastoma Protein (RB) E3 Ubiquitin Ligase (NRBE3) Promotes RB Degradation and Is Transcriptionally Regulated by E2F1 Transcription Factor
作者: Wang, Yingshuang1,2; Zheng, Zongfang1,2; Zhang, Jingyi1; Wang, You1,2; Kong, Ruirui1,2; Liu, Jiangying1,2; Zhang, Ying1,2; Deng, Hongkui1,3; Du, Xiaojuan1,3; Ke, Yang1,2
刊名: JOURNAL OF BIOLOGICAL CHEMISTRY
发表日期: 2015-11-20
DOI: 10.1074/jbc.M115.655597
卷: 290, 期:47, 页:28200-28213
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: SUSCEPTIBILITY GENE-PRODUCT ; CELL-CYCLE CONTROL ; BREAST-CANCER ; TUMOR-SUPPRESSOR ; THERAPEUTIC RESPONSE ; PROTEASOME PATHWAY ; DEPENDENT DEGRADATION ; ONCOPROTEIN MDM2 ; FAMILY PROTEINS ; E7
英文摘要:

Retinoblastoma protein (RB) plays critical roles in tumor suppression and is degraded through the proteasomal pathway. However, E3 ubiquitin ligases responsible for proteasome-mediated degradation of RB are largely unknown. Here we characterize a novel RB E3 ubiquitin ligase (NRBE3) that binds RB and promotes RB degradation. NRBE3 contains an LXCXE motif and bound RB in vitro. NRBE3 interacted with RB in cells when proteasome activity was inhibited. NRBE3 promoted RB ubiquitination and degradation via the ubiquitin-proteasome pathway. Importantly, purified NRBE3 ubiquitinated recombinant RB in vitro, and a U-box was identified as essential for its E3 activity. Surprisingly, NRBE3 was transcriptionally activated by E2F1/DP1. Consequently, NRBE3 affected the cell cycle by promoting G1/S transition. Moreover, NRBE3 was up-regulated in breast cancer tissues. Taken together, we identified NRBE3 as a novel ubiquitin E3 ligase for RB that might play a role as a potential oncoprotein in human cancers.

语种: 英语
所属项目编号: 30571038 ; 81372222 ; IRT13001 ; 7122032
项目资助者: National Natural Science Foundation of China ; Innovation team of the Ministry of Education ; Beijing Municipal Natural Science Foundation
WOS记录号: WOS:000365761900015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61606
Appears in Collections:北京大学临床肿瘤学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Beijing Canc Hosp & Inst, Sch Oncol, Key Lab Carcinogenesis & Translat Res,Minist Educ, Beijing 100142, Peoples R China
2.Peking Univ, Beijing Canc Hosp & Inst, Sch Oncol, Genet Lab, Beijing 100142, Peoples R China
3.Peking Univ, Hlth Sci Ctr, Sch Basic Med Sci, Dept Cell Biol, Beijing 100191, Peoples R China

Recommended Citation:
Wang, Yingshuang,Zheng, Zongfang,Zhang, Jingyi,et al. A Novel Retinoblastoma Protein (RB) E3 Ubiquitin Ligase (NRBE3) Promotes RB Degradation and Is Transcriptionally Regulated by E2F1 Transcription Factor[J]. JOURNAL OF BIOLOGICAL CHEMISTRY,2015,290(47):28200-28213.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Wang, Yingshuang]'s Articles
[Zheng, Zongfang]'s Articles
[Zhang, Jingyi]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Wang, Yingshuang]‘s Articles
[Zheng, Zongfang]‘s Articles
[Zhang, Jingyi]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace