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学科主题: 临床医学
题名:
WHAMM is required for meiotic spindle migration and asymmetric cytokinesis in mouse oocytes
作者: Huang, Xin1; Ding, Lu1; Pan, Rui1; Ma, Peng-Fei1; Cheng, Pan-Pan1; Zhang, Chun-Hui2; Shen, Yu-Ting3; Xu, Lin1; Liu, Yu1; He, Xiao-Qin3; Qi, Zhong-Quan1; Wang, Hai-Long1
关键词: WHAMM ; Immunofluorescence ; Meiosis ; Spindle migration ; Asymmetric cytokinesis ; Mouse oocyte
刊名: HISTOCHEMISTRY AND CELL BIOLOGY
发表日期: 2013-04-01
DOI: 10.1007/s00418-012-1051-z
卷: 139, 期:4, 页:525-534
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Microscopy
研究领域[WOS]: Cell Biology ; Microscopy
关键词[WOS]: ARP2/3 COMPLEX ; CORTICAL POLARITY ; CELL-DIVISION ; ACTIN ; MATURATION ; FERTILIZATION ; DYNAMICS ; EGGS ; MICROFILAMENTS ; CENTROSOME
英文摘要:

WASP homolog associated with actin, membranes and microtubules (WHAMM) is a newly discovered nucleation-promoting factor that links actin and microtubule cytoskeleton and regulates transport from the endoplasmic reticulum to the Golgi apparatus. However, knowledge of WHAMM is limited to interphase somatic cells. In this study, we examined its localization and function in mouse oocytes during meiosis. Immunostaining showed that in the germinal vesicle (GV) stage, there was no WHAMM signal; after meiosis resumption, WHAMM was associated with the spindle at prometaphase I (Pro MI), metaphase I (MI), telophase I (TI) and metaphase II (MII) stages. Nocodazole and taxol treatments showed that WHAMM was localized around the MI spindle. Depletion of WHAMM by microinjection of specific short interfering (si)RNA into the oocyte cytoplasm resulted in failure of spindle migration, disruption of asymmetric cytokinesis and a decrease in the first polar body extrusion rate during meiotic maturation. Moreover, actin cap formation was also disrupted after WHAMM depletion, confirming the failure of spindle migration. Taken together, our data suggest that WHAMM is required for peripheral spindle migration and asymmetric cytokinesis during mouse oocyte maturation.

语种: 英语
所属项目编号: 31201695 ; 2012CBA01303
项目资助者: National Natural Science Foundation of China ; Major State Scientific Research Program of China
WOS记录号: WOS:000316484400003
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61608
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Xiamen Univ, Organ Transplantat Inst, Xiamen, Fujian, Peoples R China
2.Peking Univ, Med Ctr, Shenzhen Hosp, Dept Reprod Med, Shenzhen, Guangdong, Peoples R China
3.Xiamen Univ, Zhongshan Hosp, Dept Gynecol & Obstet, Xiamen, Fujian, Peoples R China

Recommended Citation:
Huang, Xin,Ding, Lu,Pan, Rui,et al. WHAMM is required for meiotic spindle migration and asymmetric cytokinesis in mouse oocytes[J]. HISTOCHEMISTRY AND CELL BIOLOGY,2013,139(4):525-534.
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