IR@PKUHSC  > 北京大学深圳医院
学科主题临床医学
WHAMM is required for meiotic spindle migration and asymmetric cytokinesis in mouse oocytes
Huang, Xin1; Ding, Lu1; Pan, Rui1; Ma, Peng-Fei1; Cheng, Pan-Pan1; Zhang, Chun-Hui2; Shen, Yu-Ting3; Xu, Lin1; Liu, Yu1; He, Xiao-Qin3; Qi, Zhong-Quan1; Wang, Hai-Long1
关键词WHAMM Immunofluorescence Meiosis Spindle migration Asymmetric cytokinesis Mouse oocyte
刊名HISTOCHEMISTRY AND CELL BIOLOGY
2013-04-01
DOI10.1007/s00418-012-1051-z
139期:4页:525-534
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Microscopy
研究领域[WOS]Cell Biology ; Microscopy
关键词[WOS]ARP2/3 COMPLEX ; CORTICAL POLARITY ; CELL-DIVISION ; ACTIN ; MATURATION ; FERTILIZATION ; DYNAMICS ; EGGS ; MICROFILAMENTS ; CENTROSOME
英文摘要

WASP homolog associated with actin, membranes and microtubules (WHAMM) is a newly discovered nucleation-promoting factor that links actin and microtubule cytoskeleton and regulates transport from the endoplasmic reticulum to the Golgi apparatus. However, knowledge of WHAMM is limited to interphase somatic cells. In this study, we examined its localization and function in mouse oocytes during meiosis. Immunostaining showed that in the germinal vesicle (GV) stage, there was no WHAMM signal; after meiosis resumption, WHAMM was associated with the spindle at prometaphase I (Pro MI), metaphase I (MI), telophase I (TI) and metaphase II (MII) stages. Nocodazole and taxol treatments showed that WHAMM was localized around the MI spindle. Depletion of WHAMM by microinjection of specific short interfering (si)RNA into the oocyte cytoplasm resulted in failure of spindle migration, disruption of asymmetric cytokinesis and a decrease in the first polar body extrusion rate during meiotic maturation. Moreover, actin cap formation was also disrupted after WHAMM depletion, confirming the failure of spindle migration. Taken together, our data suggest that WHAMM is required for peripheral spindle migration and asymmetric cytokinesis during mouse oocyte maturation.

语种英语
WOS记录号WOS:000316484400003
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61608
Collection北京大学深圳医院
作者单位1.Xiamen Univ, Organ Transplantat Inst, Xiamen, Fujian, Peoples R China
2.Peking Univ, Med Ctr, Shenzhen Hosp, Dept Reprod Med, Shenzhen, Guangdong, Peoples R China
3.Xiamen Univ, Zhongshan Hosp, Dept Gynecol & Obstet, Xiamen, Fujian, Peoples R China
Recommended Citation
GB/T 7714
Huang, Xin,Ding, Lu,Pan, Rui,et al. WHAMM is required for meiotic spindle migration and asymmetric cytokinesis in mouse oocytes[J]. HISTOCHEMISTRY AND CELL BIOLOGY,2013,139(4):525-534.
APA Huang, Xin.,Ding, Lu.,Pan, Rui.,Ma, Peng-Fei.,Cheng, Pan-Pan.,...&Wang, Hai-Long.(2013).WHAMM is required for meiotic spindle migration and asymmetric cytokinesis in mouse oocytes.HISTOCHEMISTRY AND CELL BIOLOGY,139(4),525-534.
MLA Huang, Xin,et al."WHAMM is required for meiotic spindle migration and asymmetric cytokinesis in mouse oocytes".HISTOCHEMISTRY AND CELL BIOLOGY 139.4(2013):525-534.
Files in This Item:
There are no files associated with this item.
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
谷歌学术
谷歌学术Similar articles in
[Huang, Xin]'s Articles
[Ding, Lu]'s Articles
[Pan, Rui]'s Articles
百度学术
百度学术Similar articles in
[Huang, Xin]'s Articles
[Ding, Lu]'s Articles
[Pan, Rui]'s Articles
必应学术
必应学术Similar articles in
[Huang, Xin]'s Articles
[Ding, Lu]'s Articles
[Pan, Rui]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.