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WHAMM is required for meiotic spindle migration and asymmetric cytokinesis in mouse oocytes
Huang, Xin1; Ding, Lu1; Pan, Rui1; Ma, Peng-Fei1; Cheng, Pan-Pan1; Zhang, Chun-Hui2; Shen, Yu-Ting3; Xu, Lin1; Liu, Yu1; He, Xiao-Qin3; Qi, Zhong-Quan1; Wang, Hai-Long1
关键词WHAMM Immunofluorescence Meiosis Spindle migration Asymmetric cytokinesis Mouse oocyte
刊名HISTOCHEMISTRY AND CELL BIOLOGY
2013-04-01
DOI10.1007/s00418-012-1051-z
139期:4页:525-534
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Microscopy
资助者National Natural Science Foundation of China ; Major State Scientific Research Program of China ; National Natural Science Foundation of China ; Major State Scientific Research Program of China
研究领域[WOS]Cell Biology ; Microscopy
关键词[WOS]ARP2/3 COMPLEX ; CORTICAL POLARITY ; CELL-DIVISION ; ACTIN ; MATURATION ; FERTILIZATION ; DYNAMICS ; EGGS ; MICROFILAMENTS ; CENTROSOME
英文摘要

WASP homolog associated with actin, membranes and microtubules (WHAMM) is a newly discovered nucleation-promoting factor that links actin and microtubule cytoskeleton and regulates transport from the endoplasmic reticulum to the Golgi apparatus. However, knowledge of WHAMM is limited to interphase somatic cells. In this study, we examined its localization and function in mouse oocytes during meiosis. Immunostaining showed that in the germinal vesicle (GV) stage, there was no WHAMM signal; after meiosis resumption, WHAMM was associated with the spindle at prometaphase I (Pro MI), metaphase I (MI), telophase I (TI) and metaphase II (MII) stages. Nocodazole and taxol treatments showed that WHAMM was localized around the MI spindle. Depletion of WHAMM by microinjection of specific short interfering (si)RNA into the oocyte cytoplasm resulted in failure of spindle migration, disruption of asymmetric cytokinesis and a decrease in the first polar body extrusion rate during meiotic maturation. Moreover, actin cap formation was also disrupted after WHAMM depletion, confirming the failure of spindle migration. Taken together, our data suggest that WHAMM is required for peripheral spindle migration and asymmetric cytokinesis during mouse oocyte maturation.

语种英语
所属项目编号31201695 ; 2012CBA01303
资助者National Natural Science Foundation of China ; Major State Scientific Research Program of China ; National Natural Science Foundation of China ; Major State Scientific Research Program of China
WOS记录号WOS:000316484400003
引用统计
被引频次:10[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61608
专题北京大学深圳医院
作者单位1.Xiamen Univ, Organ Transplantat Inst, Xiamen, Fujian, Peoples R China
2.Peking Univ, Med Ctr, Shenzhen Hosp, Dept Reprod Med, Shenzhen, Guangdong, Peoples R China
3.Xiamen Univ, Zhongshan Hosp, Dept Gynecol & Obstet, Xiamen, Fujian, Peoples R China
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GB/T 7714
Huang, Xin,Ding, Lu,Pan, Rui,et al. WHAMM is required for meiotic spindle migration and asymmetric cytokinesis in mouse oocytes[J]. HISTOCHEMISTRY AND CELL BIOLOGY,2013,139(4):525-534.
APA Huang, Xin.,Ding, Lu.,Pan, Rui.,Ma, Peng-Fei.,Cheng, Pan-Pan.,...&Wang, Hai-Long.(2013).WHAMM is required for meiotic spindle migration and asymmetric cytokinesis in mouse oocytes.HISTOCHEMISTRY AND CELL BIOLOGY,139(4),525-534.
MLA Huang, Xin,et al."WHAMM is required for meiotic spindle migration and asymmetric cytokinesis in mouse oocytes".HISTOCHEMISTRY AND CELL BIOLOGY 139.4(2013):525-534.
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