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学科主题临床医学
Inositol-1,4,5-trisphosphate and ryanodine-dependent Ca2+ signaling in a chronic dog model of atrial fibrillation
Zhao, Zhi-Hong; Zhang, Hai-Cheng; Xu, Yuan; Zhang, Ping; Li, Xue-Bing; Liu, Yuan-Sheng; Guo, Ji-Hong
关键词atrial fibrillation ryanodine receptor calcium release inositol 1,4,5-triphosphate receptor channel signal transduction
刊名CARDIOLOGY
2007
DOI10.1159/000095517
107期:4页:269-276
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cardiac & Cardiovascular Systems
研究领域[WOS]Cardiovascular System & Cardiology
关键词[WOS]SPONTANEOUS CALCIUM-RELEASE ; SARCOPLASMIC-RETICULUM ; CARDIAC MYOCYTES ; HEART-FAILURE ; CONTRACTION ; RECEPTOR ; ARRHYTHMIAS ; GENERATION ; INSP(3) ; STROKE
英文摘要

Ca2+ signaling regulation plays an important role in triggering and/or maintaining atrial fibrillation (AF). Little is known about the relationship of the inositol-1,4,5-triphosphate receptors (InsP(3)Rs) and ryanodine receptors (RyRs) in left atrium to chronic AF. In this study, we investigated the expression and function of InsP(3)R(1), InsP(3)R(2) and RyR(2) in a chronic dog model of AF. AF was induced in 6 dogs by rapid right atrial pacing for 24 weeks, and a sham procedure was performed in 5 dogs (control group). The intact left atrial myocytes were used to examine the expression and function of InsP(3)Rs, RyRs by BODIPYO,R TR-X ryanodine, heparin-fluorescein conjugate, and were stimulated by caffeine, ATP to release Ca2+ through RyRs, InsP(3)Rs separately. We also assessed the molecular components of left atrial tissue underlying the amount of RyR(2), InsP(3)R(1) and InsP(3)R(2) determined by RT-PCR, immunohistochemistry and Western blot analysis. In the chronic AF group, the Ca2+ released through RyRs is not altered, but the Ca2+ released through InsP(3)Rs increased significantly. RyR(2) distributed in cytosol of myocytes, cellular membrane; its expression significantly decreased in AF group compared to controls. InsP(3)R(1) distributed in cytosol, InsP(3)R(2) distributed not only in cytosol, cellular membrane, but also in nuclear envelope and intercalated discs. The InsP(3)R(1) and InsP(3)R(2) expression significantly increased in chronic AF group compared to controls. These results indicated that in a chronic dog model of AF, the expression and function of RyR(2) down-regulated; on the contrary, the expression and function of InsP(3)R(1), InsP(3)R(2) up-regulated, and InsP(3)R(2) may be the major InsP(3)Rs, which regulate intracellular or even intercellular Ca2+ signal transmission.

语种英语
WOS记录号WOS:000242210400008
引用统计
被引频次:15[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61611
专题北京大学第二临床医学院_心血管内科
作者单位Peking Univ, Peoples Hosp, Dept Cardiol, Electrophysiol Grp, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Zhi-Hong,Zhang, Hai-Cheng,Xu, Yuan,et al. Inositol-1,4,5-trisphosphate and ryanodine-dependent Ca2+ signaling in a chronic dog model of atrial fibrillation[J]. CARDIOLOGY,2007,107(4):269-276.
APA Zhao, Zhi-Hong.,Zhang, Hai-Cheng.,Xu, Yuan.,Zhang, Ping.,Li, Xue-Bing.,...&Guo, Ji-Hong.(2007).Inositol-1,4,5-trisphosphate and ryanodine-dependent Ca2+ signaling in a chronic dog model of atrial fibrillation.CARDIOLOGY,107(4),269-276.
MLA Zhao, Zhi-Hong,et al."Inositol-1,4,5-trisphosphate and ryanodine-dependent Ca2+ signaling in a chronic dog model of atrial fibrillation".CARDIOLOGY 107.4(2007):269-276.
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