IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科
学科主题临床医学
CCL2 and CXCL1 trigger calcitonin gene-related peptide release by exciting primary nociceptive neurons
Qin, XM; Wan, Y; Wang, X
关键词CCL2 CXCL1 dorsal root ganglion calcitonin gene-related peptide
刊名JOURNAL OF NEUROSCIENCE RESEARCH
2005-10-01
DOI10.1002/jnr.20612
82期:1页:51-62
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Neurosciences
研究领域[WOS]Neurosciences & Neurology
关键词[WOS]PROTEIN-KINASE-C ; CHEMOKINE RECEPTOR CXCR2 ; DORSAL-ROOT GANGLION ; SIGNAL-TRANSDUCTION ; ALZHEIMERS-DISEASE ; OPIOID RECEPTORS ; NEUROPATHIC PAIN ; CALCIUM-IONS ; SUBSTANCE-P ; RAT SKIN
英文摘要

Chemokines are important mediators in immune responses and inflammatory processes. Calcitonin gene-related peptide (CGRP) is produced in dorsal root ganglion (DRG) neurons. In this study, CGRP radioimmunoassay was used to investigate whether the chemokines CCL2 and CXCL1 could trigger CGRP release from cultured DRG neurons of neonatal rats and, if so, which cellular signaling pathway was involved. The results showed that CCL2 and CXCL1 (similar to 5-100 ng/ml) evoked CGRP release and intracellular calcium elevation in a pertussis toxin (PTX)-sensitive manner. The CGRP release by CCL2 and CXCL1 was significantly inhibited by EGTA, omega-conotoxin GVIA (an N-type calcium channel blocker), thapsigargin, and ryanodine. Pretreatment of DRG neurons for 30 min with the inhibitors of phospholipase C (PLC) and protein kinase C (PKC) but not mitogen-activated protein kinases (MAPKs) significantly reduced CCL2- or CXCL1-induced CGRP release and intracellular calcium elevation. Intraplantar injection of CCL2 or CXCL1 produced hyperalgesia to thermal and mechanical stimulation in rats. These data suggest that CCL2 and CXCL1 can stimulate CGRP release and intracellular calcium elevation in DRG neurons. PLC-, PKC-, and calcium-induced calcium release from ryanodine-sensitive calcium stores signaling pathways are involved in CCL2- and CXCL1-induced CGRP release from primary nociceptive neurons, in which chemokines produce painful effects via direct actions on chemokine receptors expressed by nociceptive neurons. (C) 2005 Wiley-Liss, Inc.

语种英语
WOS记录号WOS:000232431300006
Citation statistics
Cited Times:88[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61612
Collection北京大学第三临床医学院_心血管内科
作者单位1.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China
2.Peking Univ, Basic Med Coll, Neurosci Res Inst, Beijing 100871, Peoples R China
3.Peking Univ, Basic Med Coll, Dept Physiol, Beijing 100871, Peoples R China
4.Peking Univ, Basic Med Coll, Educ Minist, Key Lab Mol Cardiovasc Sci, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Qin, XM,Wan, Y,Wang, X. CCL2 and CXCL1 trigger calcitonin gene-related peptide release by exciting primary nociceptive neurons[J]. JOURNAL OF NEUROSCIENCE RESEARCH,2005,82(1):51-62.
APA Qin, XM,Wan, Y,&Wang, X.(2005).CCL2 and CXCL1 trigger calcitonin gene-related peptide release by exciting primary nociceptive neurons.JOURNAL OF NEUROSCIENCE RESEARCH,82(1),51-62.
MLA Qin, XM,et al."CCL2 and CXCL1 trigger calcitonin gene-related peptide release by exciting primary nociceptive neurons".JOURNAL OF NEUROSCIENCE RESEARCH 82.1(2005):51-62.
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