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学科主题: 临床医学
题名:
CCL2 and CXCL1 trigger calcitonin gene-related peptide release by exciting primary nociceptive neurons
作者: Qin, XM; Wan, Y; Wang, X
关键词: CCL2 ; CXCL1 ; dorsal root ganglion ; calcitonin gene-related peptide
刊名: JOURNAL OF NEUROSCIENCE RESEARCH
发表日期: 2005-10-01
DOI: 10.1002/jnr.20612
卷: 82, 期:1, 页:51-62
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Neurosciences
研究领域[WOS]: Neurosciences & Neurology
关键词[WOS]: PROTEIN-KINASE-C ; CHEMOKINE RECEPTOR CXCR2 ; DORSAL-ROOT GANGLION ; SIGNAL-TRANSDUCTION ; ALZHEIMERS-DISEASE ; OPIOID RECEPTORS ; NEUROPATHIC PAIN ; CALCIUM-IONS ; SUBSTANCE-P ; RAT SKIN
英文摘要:

Chemokines are important mediators in immune responses and inflammatory processes. Calcitonin gene-related peptide (CGRP) is produced in dorsal root ganglion (DRG) neurons. In this study, CGRP radioimmunoassay was used to investigate whether the chemokines CCL2 and CXCL1 could trigger CGRP release from cultured DRG neurons of neonatal rats and, if so, which cellular signaling pathway was involved. The results showed that CCL2 and CXCL1 (similar to 5-100 ng/ml) evoked CGRP release and intracellular calcium elevation in a pertussis toxin (PTX)-sensitive manner. The CGRP release by CCL2 and CXCL1 was significantly inhibited by EGTA, omega-conotoxin GVIA (an N-type calcium channel blocker), thapsigargin, and ryanodine. Pretreatment of DRG neurons for 30 min with the inhibitors of phospholipase C (PLC) and protein kinase C (PKC) but not mitogen-activated protein kinases (MAPKs) significantly reduced CCL2- or CXCL1-induced CGRP release and intracellular calcium elevation. Intraplantar injection of CCL2 or CXCL1 produced hyperalgesia to thermal and mechanical stimulation in rats. These data suggest that CCL2 and CXCL1 can stimulate CGRP release and intracellular calcium elevation in DRG neurons. PLC-, PKC-, and calcium-induced calcium release from ryanodine-sensitive calcium stores signaling pathways are involved in CCL2- and CXCL1-induced CGRP release from primary nociceptive neurons, in which chemokines produce painful effects via direct actions on chemokine receptors expressed by nociceptive neurons. (C) 2005 Wiley-Liss, Inc.

语种: 英语
WOS记录号: WOS:000232431300006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61612
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

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作者单位: 1.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100083, Peoples R China
2.Peking Univ, Basic Med Coll, Neurosci Res Inst, Beijing 100871, Peoples R China
3.Peking Univ, Basic Med Coll, Dept Physiol, Beijing 100871, Peoples R China
4.Peking Univ, Basic Med Coll, Educ Minist, Key Lab Mol Cardiovasc Sci, Beijing 100871, Peoples R China

Recommended Citation:
Qin, XM,Wan, Y,Wang, X. CCL2 and CXCL1 trigger calcitonin gene-related peptide release by exciting primary nociceptive neurons[J]. JOURNAL OF NEUROSCIENCE RESEARCH,2005,82(1):51-62.
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