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学科主题: 临床医学
题名:
Soluble fusion expression, characterization and localization of human beta-defensin 6
作者: Xin, Aijie1; Zhao, Yue4; Yu, Heguo3; Shi, Huijuan3; Liu, Haiyan5; Diao, Hua3; Zhang, Yonglian1,2,3
关键词: human beta-defensin 6 ; antimicrobial peptide ; intein ; purification ; binding with heparin and lipopolysaccharide
刊名: MOLECULAR MEDICINE REPORTS
发表日期: 2014
DOI: 10.3892/mmr.2013.1768
卷: 9, 期:1, 页:149-155
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology ; Medicine, Research & Experimental
研究领域[WOS]: Oncology ; Research & Experimental Medicine
关键词[WOS]: ESCHERICHIA-COLI ; 2 HBD2 ; PEPTIDES ; BINDING ; IDENTIFICATION ; EVOLUTION ; MEMBRANES ; INSERTION ; LIGATION ; PROTEINS
英文摘要:

Human -defensin 6 (DEFB106) is an antimicrobial peptide expressed in the epididymis, testis and lung, which indicates that DEFB106 may be involved in innate immunity and fertility. However, as a -defensin, this protein has not been well characterized. Using an intein-mediated fusion expression system, the recombinant DEFB106 was expressed and purified (yield, 3-5 mg/l) under optimized conditions. The purified protein was characterized using mass spectrometry and circular dichroism spectroscopy. The measured molecular weight was consistent with its theoretical value and the predominant secondary structure was -sheet, the common structure of -defensin family members. The purified DEFB106 showed antimicrobial activity against not only Escherichia coli (E. coli) and Candida albicans (C. albicans) SC5314, but also Staphylococcus aureus (S. aureus) CMCC26003. Furthermore, it exhibited a high affinity for heparin and lipopolysaccharide. In addition, it was determined that native DEFB106 was located in the epididymis, bone marrow and skin. These observations may aid in the determination of the physiological and pathological functions of DEFB106.

语种: 英语
所属项目编号: 31101030 ; 81270744 ; 30930053 ; KSCX2-EW-R-07
项目资助者: Natural Science Foundation of China ; Chinese Academy of Sciences
WOS记录号: WOS:000330783700024
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61615
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.E China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China
2.Chinese Acad Sci, Shanghai Inst Biol Sci, Inst Biochem & Cell Biol, Shanghai Key Lab Mol Androl,State Key Lab Mol Bio, Shanghai 200031, Peoples R China
3.Shanghai Inst Planned Parenthood Res, NPFPC Key Lab Contracept & Devices, Shanghai 200032, Peoples R China
4.Peking Univ, Hosp 3, Dept Obstet & Gynecol, Reprod Med Ctr, Beijing 100191, Peoples R China
5.China Pharmaceut Univ, Sch Life Sci & Technol, Nanjing 210009, Peoples R China

Recommended Citation:
Xin, Aijie,Zhao, Yue,Yu, Heguo,et al. Soluble fusion expression, characterization and localization of human beta-defensin 6[J]. MOLECULAR MEDICINE REPORTS,2014,9(1):149-155.
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