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学科主题: 临床医学
题名:
Identification and characterization of human PCDH10 gene promoter
作者: Li, Zesong1; Xie, Jun1; Li, Wenjie1; Tang, Aifa1; Li, Xianxin1; Jiang, Zhimao1; Han, Yonghua1; Ye, Jiongxian1; Jing, Jie2; Gui, Yaoting1; Cai, Zhiming1,3
关键词: TATA-less promoter ; NF-Y ; Sp1/Sp3 ; CAAT box
刊名: GENE
发表日期: 2011-04-01
DOI: 10.1016/j.gene.2011.01.001
卷: 475, 期:1, 页:49-56
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Genetics & Heredity
研究领域[WOS]: Genetics & Heredity
关键词[WOS]: CANDIDATE TUMOR-SUPPRESSOR ; CADHERIN SUPERFAMILY ; TRANSCRIPTION FACTOR ; NF-Y ; PROTOCADHERIN FAMILY ; SP1 ; EXPRESSION ; BINDING ; METHYLATION ; CANCER
英文摘要:

Recent studies have suggested roles for PCDH10 as a novel tumor suppressor gene. In our previous work, we located the core promoter of PCDH10 to a 462-bp segment of 5′-flanking region characterized by a high GC content. Here we further identified and characterized the promoter for PCDH10. Transient transfection of PC3 and LNCaP cells with a series of deleted promoter constructs indicated that the minimal promoter region was between nucleotides - 144 and -99. This segment contained a CAAT box, a GT box, and a putative transcription factor binding site for AP-4. Mutational analysis identified that the CAAT box and GT box are necessary for promoter activity. Ectopic expression of NF-Ys increased reporter gene activity, whereas expression of a dominant-negative NF-YA decreased reporter gene activity. Co-transfection of Sp1/Sp3 expression plasmids enhanced reporter gene activity in a dose-dependent manner. Mithramycin A. an inhibitor of Sp-DNA interaction, reduced PCDH10 promoter activity. Electrophoretic mobility shift assays and chromatin immunoprecipitation demonstrated binding of transcription factors Sp1/Sp3 to the promoter region in vitro and in vivo. Our data show that Spl/Sp3 and CBF/NF-Y transcription factors play a crucial role in the basal expression of the human PCDH10 gene. (C) 2011 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 30700824 ; 30972992 ; 200800010106 ; A2010548
项目资助者: National Natural Science Foundation of China ; Research Fund for the Doctoral Program of Higher Education of China ; Medical Scientific Research Foundation of Guangdong Province ; Scientific Research Foundation of the State Human Resource Ministry for Returned Chinese Scholars, China
WOS记录号: WOS:000288936100006
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61624
Appears in Collections:北京大学深圳医院_期刊论文

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作者单位: 1.Peking Univ Shenzhen Hosp, Shenzhen PKU HKUST Med Ctr, Guangdong Key Lab Male Reprod Med & Genet, Shenzhen 518036, Guangdong, Peoples R China
2.Peking Univ, Peking Univ Hosp 1, Beijing 100034, Peoples R China
3.Shenzhen Univ, Affiliated Hosp 1, Shenzhen Peoples Hosp 2, Shenzhen 518035, Peoples R China

Recommended Citation:
Li, Zesong,Xie, Jun,Li, Wenjie,et al. Identification and characterization of human PCDH10 gene promoter[J]. GENE,2011,475(1):49-56.
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