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学科主题: 公共卫生
题名:
Connexin43 Hemichannels Contribute to Cadmium-Induced Oxidative Stress and Cell Injury
作者: Fang, Xin2; Huang, Tao; Zhu, Ying; Yan, Qiaojing; Chi, Yuan; Jiang, Jean X.3; Wang, Peiyu4; Matsue, Hiroyuki5; Kitamura, Masanori; Yao, Jian1
刊名: ANTIOXIDANTS & REDOX SIGNALING
发表日期: 2011-06-01
DOI: 10.1089/ars.2010.3150
卷: 14, 期:12, 页:2427-2439
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Endocrinology & Metabolism
研究领域[WOS]: Biochemistry & Molecular Biology ; Endocrinology & Metabolism
关键词[WOS]: JUNCTIONAL INTERCELLULAR COMMUNICATION ; UNFOLDED PROTEIN RESPONSE ; GAP-JUNCTION ; INDUCED APOPTOSIS ; OXIDANT STRESS ; LLC-PK1 CELLS ; ATP RELEASE ; E-CADHERIN ; ACTIVATION ; ASTROCYTES
英文摘要:

We investigated the potential involvement of connexin hemichannels in cadmium ions (Cd2+)-elicited cell injury. Transfection of LLC-PK1 cells with a wild-type connexin43 (Cx43) sensitized them to Cd2+-elicited cell injury. The cell susceptibility to Cd2+ was increased by depletion of glutathione (GSH) with DL-buthionine-[S, R]sulfoximine, and decreased by N-acetyl-cysteine or glutathione reduced ethyl ester. Fibroblasts derived from Cx43 wild-type (Cx43+/+) and knockout (Cx43-/-) fetal littermates displayed different susceptibility to Cd2+. Cd2+ induced a higher concentration of reactive oxygen species, a stronger activation c-Jun N-terminal kinase, and significantly more severe cell injury in Cx43+/+ fibroblasts, as compared with Cx43-/-fibroblasts. Cd2+ caused a reduction in intracellular GSH, whereas it elevated extracellular GSH. This effect of Cd2+ was more dramatic in Cx43+/+ than Cx43-/- fibroblasts. Treatment of Cx43+/+ fibroblasts with Cd2+ caused a Cx43 hemichannel-dependent influx of Lucifer Yellow and efflux of ATP. Collectively, our study demonstrates that Cx43 sensitizes cells to Cd2+-initiated cytotoxicity, possibly through hemichannel-mediated effects on intracellular oxidative status. Antioxid. Redox Signal. 14, 2427-2439.

语种: 英语
所属项目编号: 17659255 ; 20590953 ; B21390324 ; AR 46798 ; AQ-1507
项目资助者: Ministry of Education, Culture, Sports, Science and Technology, Japan ; Takeda Science Foundation ; Japan-China Medical Association ; University of Yamanashi ; National Institute of Health ; Welch Foundation
WOS记录号: WOS:000290783700009
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61631
Appears in Collections:北京大学公共卫生学院_期刊论文

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作者单位: 1.Chiba Univ, Sch Med, Dept Dermatol, Chiba 280, Japan
2.Univ Yamanashi, Interdisciplinary Grad Sch Med & Engn, Dept Mol Signaling, Chuo Ku, Yamanashi 4093898, Japan
3.Inner Mongolia Med Coll, Sch Basic Med Sci, Hohhot, Peoples R China
4.Univ Texas Hlth Sci Ctr San Antonio, Dept Biochem, San Antonio, TX 78229 USA
5.Peking Univ, Sch Publ Hlth, Dept Social Med & Hlth Educ, Beijing 100871, Peoples R China

Recommended Citation:
Fang, Xin,Huang, Tao,Zhu, Ying,et al. Connexin43 Hemichannels Contribute to Cadmium-Induced Oxidative Stress and Cell Injury[J]. ANTIOXIDANTS & REDOX SIGNALING,2011,14(12):2427-2439.
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