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Identification of chemicals and their metabolites from PHY906, a Chinese medicine formulation, in the plasma of a patient treated with irinotecan and PHY906 using liquid chromatography/tandem mass spectrometry (LC/MS/MS)
Zhang, Wei1,2; Saif, Muhammad W.2,3; Dutschman, Ginger E.1,2; Li, Xin2; Lam, Wing1; Bussom, Scott1,2; Jiang, Zaoli1; Ye, Min1,4; Chu, Edward2,3; Cheng, Yung-Chi1,2
关键词LC/MS/MS Pharmacokinetics Traditional Chinese Medicine Metabolites
刊名JOURNAL OF CHROMATOGRAPHY A
2010-09-10
DOI10.1016/j.chroma.2010.07.045
1217期:37页:5785-5793
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]OCTANOL/WATER PARTITION-COEFFICIENTS ; RAT PLASMA ; BAICALIN ; EXTRACT ; PREDICTION
英文摘要

Traditional Chinese Medicine (TCM) is increasingly being used in combination with Western medicine. In general, TCM is comprised of multiple components in sharp contrast to Western medicine, where a single active chemical is used. Presently, there are no well-established standards for most of the chemical compounds of TCM and their respective metabolites. Moreover, there are no formal analytical methods for the identification of these chemicals, especially in trace amounts. The ability to measure the pharmacokinetic behaviors of chemicals and their metabolites from these herbal formulations are critical in understanding of the action of TCM. This paper describes the use of LC/MS/MS along with enzyme treatments and n-octanol/water partition coefficient, to investigate the chemical components of PHY906 and their metabolites in the plasma of a patient with metastatic colorectal cancer (mCRC) treated with irinotecan and PHY906. The chemicals from an aqueous extract of PHY906 and the plasma from a patient was prepared and separated on an Agilent ZORBAX-SB C(18) column, and eluted with acetonitrile/0.05% (v/v) formic acid. From the PHY906 aqueous extract, a total of 57 compounds and 27 metabolites were identified and tentatively assigned structures based on their identified mass spectrometry, enzyme digestion and n-octanol/water partition coefficient. In contrast, analysis of patient plasma identified only 33 chemicals and new metabolites. These findings demonstrated that LC/MS/MS was and effective and reliable method for studying the parent chemicals of the Chinese herbal medicine PHY906 and their metabolites in a patient with metastatic colorectal cancer. (C) 2010 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000282147400007
项目编号U01CA63477 ; P30CA16359
资助机构NIH
引用统计
被引频次:41[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61634
专题北京大学药学院
北京大学药学院_天然药物学系
作者单位1.Yale Univ, Sch Med, Dept Pharmacol, New Haven, CT 06520 USA
2.Yale Univ, Sch Med, Yale Canc Ctr, Dev Therapeut Program, New Haven, CT 06520 USA
3.Yale Univ, Sch Med, Dept Med, Sect Med Oncol, New Haven, CT 06520 USA
4.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Wei,Saif, Muhammad W.,Dutschman, Ginger E.,et al. Identification of chemicals and their metabolites from PHY906, a Chinese medicine formulation, in the plasma of a patient treated with irinotecan and PHY906 using liquid chromatography/tandem mass spectrometry (LC/MS/MS)[J]. JOURNAL OF CHROMATOGRAPHY A,2010,1217(37):5785-5793.
APA Zhang, Wei.,Saif, Muhammad W..,Dutschman, Ginger E..,Li, Xin.,Lam, Wing.,...&Cheng, Yung-Chi.(2010).Identification of chemicals and their metabolites from PHY906, a Chinese medicine formulation, in the plasma of a patient treated with irinotecan and PHY906 using liquid chromatography/tandem mass spectrometry (LC/MS/MS).JOURNAL OF CHROMATOGRAPHY A,1217(37),5785-5793.
MLA Zhang, Wei,et al."Identification of chemicals and their metabolites from PHY906, a Chinese medicine formulation, in the plasma of a patient treated with irinotecan and PHY906 using liquid chromatography/tandem mass spectrometry (LC/MS/MS)".JOURNAL OF CHROMATOGRAPHY A 1217.37(2010):5785-5793.
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