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学科主题: 药学
题名:
Identification of chemicals and their metabolites from PHY906, a Chinese medicine formulation, in the plasma of a patient treated with irinotecan and PHY906 using liquid chromatography/tandem mass spectrometry (LC/MS/MS)
作者: Zhang, Wei1,2; Saif, Muhammad W.2,3; Dutschman, Ginger E.1,2; Li, Xin2; Lam, Wing1; Bussom, Scott1,2; Jiang, Zaoli1; Ye, Min1,4; Chu, Edward2,3; Cheng, Yung-Chi1,2
关键词: LC/MS/MS ; Pharmacokinetics ; Traditional Chinese Medicine ; Metabolites
刊名: JOURNAL OF CHROMATOGRAPHY A
发表日期: 2010-09-10
DOI: 10.1016/j.chroma.2010.07.045
卷: 1217, 期:37, 页:5785-5793
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Chemistry, Analytical
研究领域[WOS]: Biochemistry & Molecular Biology ; Chemistry
关键词[WOS]: OCTANOL/WATER PARTITION-COEFFICIENTS ; RAT PLASMA ; BAICALIN ; EXTRACT ; PREDICTION
英文摘要:

Traditional Chinese Medicine (TCM) is increasingly being used in combination with Western medicine. In general, TCM is comprised of multiple components in sharp contrast to Western medicine, where a single active chemical is used. Presently, there are no well-established standards for most of the chemical compounds of TCM and their respective metabolites. Moreover, there are no formal analytical methods for the identification of these chemicals, especially in trace amounts. The ability to measure the pharmacokinetic behaviors of chemicals and their metabolites from these herbal formulations are critical in understanding of the action of TCM. This paper describes the use of LC/MS/MS along with enzyme treatments and n-octanol/water partition coefficient, to investigate the chemical components of PHY906 and their metabolites in the plasma of a patient with metastatic colorectal cancer (mCRC) treated with irinotecan and PHY906. The chemicals from an aqueous extract of PHY906 and the plasma from a patient was prepared and separated on an Agilent ZORBAX-SB C(18) column, and eluted with acetonitrile/0.05% (v/v) formic acid. From the PHY906 aqueous extract, a total of 57 compounds and 27 metabolites were identified and tentatively assigned structures based on their identified mass spectrometry, enzyme digestion and n-octanol/water partition coefficient. In contrast, analysis of patient plasma identified only 33 chemicals and new metabolites. These findings demonstrated that LC/MS/MS was and effective and reliable method for studying the parent chemicals of the Chinese herbal medicine PHY906 and their metabolites in a patient with metastatic colorectal cancer. (C) 2010 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: U01CA63477 ; P30CA16359
项目资助者: NIH
WOS记录号: WOS:000282147400007
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61634
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Yale Univ, Sch Med, Dept Pharmacol, New Haven, CT 06520 USA
2.Yale Univ, Sch Med, Yale Canc Ctr, Dev Therapeut Program, New Haven, CT 06520 USA
3.Yale Univ, Sch Med, Dept Med, Sect Med Oncol, New Haven, CT 06520 USA
4.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100871, Peoples R China

Recommended Citation:
Zhang, Wei,Saif, Muhammad W.,Dutschman, Ginger E.,et al. Identification of chemicals and their metabolites from PHY906, a Chinese medicine formulation, in the plasma of a patient treated with irinotecan and PHY906 using liquid chromatography/tandem mass spectrometry (LC/MS/MS)[J]. JOURNAL OF CHROMATOGRAPHY A,2010,1217(37):5785-5793.
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