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学科主题: 药学
题名:
Rapid characterization of chemical constituents and rats metabolites of the traditional Chinese patent medicine Gegen-Qinlian-Wan by UHPLC/DAD/qTOF-MS
作者: Miao, Wen-juan1; Wang, Qing1; Bo, Tao2; Ye, Min1; Qiao, Xue1; Yang, Wen-zhi1; Xiang, Cheng1; Guan, Xiang-yu1; Guo, De-an1
关键词: Gegen-Qinlian-Wan ; UHPLC-DAD-qTOF-MS ; Chemical constituents ; Metabolites identification ; Traditional Chinese patent medicine
刊名: JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
发表日期: 2013-01-18
DOI: 10.1016/j.jpba.2012.09.015
卷: 72, 页:99-108
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]: Chemistry ; Pharmacology & Pharmacy
关键词[WOS]: PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; COUNTER-CURRENT CHROMATOGRAPHY ; DIODE-ARRAY DETECTION ; SCUTELLARIA-BAICALENSIS ; DISPENSING GRANULE ; ACTIVE COMPONENTS ; COPTIDIS RHIZOMA ; IN-VIVO ; IDENTIFICATION
英文摘要:

Gegen-Qinlian-Wan (GQW) is a popular traditional Chinese patent medicine for the treatment of diarrhea. It is composed of four herbal medicines, Puerariae Radix, Scutellariae Radix, Coptidis Rhizoma, and Glycyrrhizae Radix. In this study, a rapid and sensitive method based on ultra high-performance liquid chromatography coupled with diode-array detection and quadrupole time-of-flight mass spectrometry (UHPLC-DAD-qTOF-MS) was established to characterize the chemical constituents and rats metabolites of GQW. Samples were separated on an Agilent Zorbax Eclipse Plus-C-18 column (100 mm x 2.1 mm, 1.8 mu m) by gradient elution using acetonitrile and water containing 0.1% formic acid as the mobile phase. On the basis of UV and qTOF high-accuracy mass spectral analysis, a total of 62 compounds were identified or tentatively characterized from GQW, including 42 flavonoids, 8 alkaloids, 6 triterpenoids, 3 phenylethanoid glycosides, and 3 other types. Among them, 27 compounds were confirmed by comparing with reference standards. Furthermore, metabolites in rats plasma and urine after oral administration of GQW were also analyzed. A total of 42 compounds were identified, including 29 prototypes and 13 metabolites through metabolic pathways of demethylation, methylation, hydrolysis, sulfate conjugation, and glucuronide conjugation. Glucuronidated flavonoids were the main constituents in the plasma, and were then transformed into aglycones and excreted from urine. This is the first systematic study on the chemical constituents and metabolic profiling of GQW. (C) 2012 Elsevier B.V. All rights reserved.

语种: 英语
所属项目编号: 81173644 ; 81222054 ; BMU20110269
项目资助者: National Natural Science Foundation of China ; Program for New Century Excellent Talents in University from China Ministry of Education
WOS记录号: WOS:000311819100013
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61643
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Agilent Technol, Beijing 100102, Peoples R China

Recommended Citation:
Miao, Wen-juan,Wang, Qing,Bo, Tao,et al. Rapid characterization of chemical constituents and rats metabolites of the traditional Chinese patent medicine Gegen-Qinlian-Wan by UHPLC/DAD/qTOF-MS[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2013,72:99-108.
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