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Rapid characterization of chemical constituents and rats metabolites of the traditional Chinese patent medicine Gegen-Qinlian-Wan by UHPLC/DAD/qTOF-MS
Miao, Wen-juan1; Wang, Qing1; Bo, Tao2; Ye, Min1; Qiao, Xue1; Yang, Wen-zhi1; Xiang, Cheng1; Guan, Xiang-yu1; Guo, De-an1
关键词Gegen-Qinlian-Wan UHPLC-DAD-qTOF-MS Chemical constituents Metabolites identification Traditional Chinese patent medicine
刊名JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
2013-01-18
DOI10.1016/j.jpba.2012.09.015
72页:99-108
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Chemistry, Analytical ; Pharmacology & Pharmacy
研究领域[WOS]Chemistry ; Pharmacology & Pharmacy
关键词[WOS]PERFORMANCE LIQUID-CHROMATOGRAPHY ; TANDEM MASS-SPECTROMETRY ; COUNTER-CURRENT CHROMATOGRAPHY ; DIODE-ARRAY DETECTION ; SCUTELLARIA-BAICALENSIS ; DISPENSING GRANULE ; ACTIVE COMPONENTS ; COPTIDIS RHIZOMA ; IN-VIVO ; IDENTIFICATION
英文摘要

Gegen-Qinlian-Wan (GQW) is a popular traditional Chinese patent medicine for the treatment of diarrhea. It is composed of four herbal medicines, Puerariae Radix, Scutellariae Radix, Coptidis Rhizoma, and Glycyrrhizae Radix. In this study, a rapid and sensitive method based on ultra high-performance liquid chromatography coupled with diode-array detection and quadrupole time-of-flight mass spectrometry (UHPLC-DAD-qTOF-MS) was established to characterize the chemical constituents and rats metabolites of GQW. Samples were separated on an Agilent Zorbax Eclipse Plus-C-18 column (100 mm x 2.1 mm, 1.8 mu m) by gradient elution using acetonitrile and water containing 0.1% formic acid as the mobile phase. On the basis of UV and qTOF high-accuracy mass spectral analysis, a total of 62 compounds were identified or tentatively characterized from GQW, including 42 flavonoids, 8 alkaloids, 6 triterpenoids, 3 phenylethanoid glycosides, and 3 other types. Among them, 27 compounds were confirmed by comparing with reference standards. Furthermore, metabolites in rats plasma and urine after oral administration of GQW were also analyzed. A total of 42 compounds were identified, including 29 prototypes and 13 metabolites through metabolic pathways of demethylation, methylation, hydrolysis, sulfate conjugation, and glucuronide conjugation. Glucuronidated flavonoids were the main constituents in the plasma, and were then transformed into aglycones and excreted from urine. This is the first systematic study on the chemical constituents and metabolic profiling of GQW. (C) 2012 Elsevier B.V. All rights reserved.

语种英语
WOS记录号WOS:000311819100013
引用统计
被引频次:39[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61643
专题北京大学药学院
北京大学药学院_天然药物学系
北京大学精神卫生研究所_药房
作者单位1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
2.Agilent Technol, Beijing 100102, Peoples R China
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GB/T 7714
Miao, Wen-juan,Wang, Qing,Bo, Tao,et al. Rapid characterization of chemical constituents and rats metabolites of the traditional Chinese patent medicine Gegen-Qinlian-Wan by UHPLC/DAD/qTOF-MS[J]. JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,2013,72:99-108.
APA Miao, Wen-juan.,Wang, Qing.,Bo, Tao.,Ye, Min.,Qiao, Xue.,...&Guo, De-an.(2013).Rapid characterization of chemical constituents and rats metabolites of the traditional Chinese patent medicine Gegen-Qinlian-Wan by UHPLC/DAD/qTOF-MS.JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS,72,99-108.
MLA Miao, Wen-juan,et al."Rapid characterization of chemical constituents and rats metabolites of the traditional Chinese patent medicine Gegen-Qinlian-Wan by UHPLC/DAD/qTOF-MS".JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS 72(2013):99-108.
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