|Differential Deposition of C4d and MBL in Glomeruli of Patients with ANCA-Negative Pauci-Immune Crescentic Glomerulonephritis|
|Xing, Guang-qun1,2; Chen, Min1; Liu, Gang1; Zheng, Xin1; E, Jie1; Zhao, Ming-hui1|
|关键词||ANCA-negative complement histopathology pauci-immune crescentic glomerulonephritis|
|刊名||JOURNAL OF CLINICAL IMMUNOLOGY|
|WOS标题词||Science & Technology|
|关键词[WOS]||LECTIN COMPLEMENT PATHWAY ; ANTINEUTROPHIL CYTOPLASMIC AUTOANTIBODIES ; RAPIDLY PROGRESSIVE GLOMERULONEPHRITIS ; RENAL INVOLVEMENT ; ACTIVATION ; VASCULITIS ; NEPHRITIS ; DISEASE ; PROTEIN ; MYELOPEROXIDASE|
Our previous study suggested involvement of alternative pathway activation of complement in ANCA-positive pauci-immune crescentic glomerulonephritis (CrGN). This study was to investigate the evidence of complement activation in renal biopsy specimens of patients with ANCA-negative pauci-immune CrGN.
Renal biopsy specimens from 12 patients with ANCA-negative pauci-immune CrGN were used to detect the staining of membrane attack complex (MAC), C3d, C4d, mannose-binding lectin (MBL), factor B by immunohistochemistry, and/or immunofluorescence. Renal tissue from eight patients with minimal change disease (MCD) and renal tissue obtained from a normal kidney and the normal parts of a nephrectomized kidney due to renal carcinoma was used as disease and normal controls, respectively.
MAC and C3d could be detected in the active renal lesions of cellular crescents in each of the 12 ANCA-negative CrGN patients but not or scarcely detected in patients with MCD and in normal renal tissue. The deposition of other complement components in the 12 patients was heterogeneous. Although none of them had C1q staining, eight of the 12 were C4d positive and six out of the eight were MBL positive. The remaining four only had evidence of the alternative pathway activation by positive staining of C3d, factor B, and MAC. The staining intensity of C4d and MBL was much higher in dialysis-dependent patients than that in dialysis-independent patients.
Complement activation was involved in renal damage of human ANCA-negative pauci-immune CrGN but with heterogeneous activation pathways. Positive staining of C4d and MBL might be associated with poor renal outcome.
|项目编号||30725034 ; 30972733|
|资助机构||National Science Fund for Distinguished Young Scholars ; National Natural Science Fund|
|作者单位||1.Peking Univ, Inst Nephrol, Div Renal, Dept Med,Key Lab Renal Dis,Minst Hlth China,Hosp, Beijing 100034, Peoples R China|
2.Qingdao Univ, Affiliated Hosp, Div Renal, Dept Med,Med Coll, Qingdao 266003, Peoples R China
|Xing, Guang-qun,Chen, Min,Liu, Gang,et al. Differential Deposition of C4d and MBL in Glomeruli of Patients with ANCA-Negative Pauci-Immune Crescentic Glomerulonephritis[J]. JOURNAL OF CLINICAL IMMUNOLOGY,2010,30(1):144-156.|
|APA||Xing, Guang-qun,Chen, Min,Liu, Gang,Zheng, Xin,E, Jie,&Zhao, Ming-hui.(2010).Differential Deposition of C4d and MBL in Glomeruli of Patients with ANCA-Negative Pauci-Immune Crescentic Glomerulonephritis.JOURNAL OF CLINICAL IMMUNOLOGY,30(1),144-156.|
|MLA||Xing, Guang-qun,et al."Differential Deposition of C4d and MBL in Glomeruli of Patients with ANCA-Negative Pauci-Immune Crescentic Glomerulonephritis".JOURNAL OF CLINICAL IMMUNOLOGY 30.1(2010):144-156.|