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学科主题: 药学
题名:
A continued study on the stealth liposomal topotecan plus amlodipine: In vitro and in vivo characterization in non-resistant solid tumors
作者: Zhang, Yu-Teng1; Lu, Wei1,2; Li, Ting1; Liang, Gong-Wen1; Sun, Jia-Bei1; Guo, Jia1; Men, Ying1; Du, Ju1; Lu, Wan-Liang1,2
关键词: stealth liposomal topotecan plus amlodipine ; solid tumors ; anti-tumor activity ; murine sarcoma cell line S180 ; human breast cancer cell line MCF-7
刊名: JOURNAL OF HEALTH SCIENCE
发表日期: 2008-08-01
卷: 54, 期:4, 页:450-463
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
关键词[WOS]: BREAST-CANCER CELLS ; LONG-CIRCULATING LIPOSOMES ; CARCINOMA A431 CELLS ; CA2+ CHANNEL BLOCKER ; MULTIDRUG-RESISTANCE ; LIPID NANOPARTICLES ; ANTITUMOR EFFICACY ; DRUG-RESISTANCE ; DELIVERY ; DOXORUBICIN
英文摘要:

We have previously established a type of anti-resistant stealth liposomal topotecan plus amlodipine for over-coming the multi-drug resistance (MDR) in resistant leukemia cells. The objective of the present study was to further characterize it in the diversified non-resistant solid tumors in vitro and in vivo. Stealth liposomal topotecan plus amlodipine was re-prepared and physicochemically characterized. The in vitro drug release assays of topotecan and amlodipine from liposomes were performed using a dialysis method. 3-(4.5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays were performed in murine sarcoma S 180 cells and human breast cancer MCF-7 cells, respectively. Apoptotic percentages of S 180 cells were evaluated using flow cytometry. In vitro anti-tumor activity study and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) analysis were performed in male Institute of Cancer Research (ICR) mice with S180 xenografts. Stealth liposomal topotecan plus amlodipine exhibited high drug encapsulation efficiencies, suitable particle size distribution, negatively charged zeta potential and prolonged release profiles for both topotecan and amlodipine. Amlodipine potentiated the antiproliferative effect and inducing apoptotic effect of topotecan on the tumor cells, exhibiting an additive anti-tumor effect. Stealth liposomal topotecan Plus amlodipine showed the optimal anti-tumor activity and inducing apoptotic effect in the in vivo studies. Stealth liposomal topotecan plus amlodipine demonstrated an overt anti-tumor activity in non-resistant solid tumors, suggesting that it deserves further clinical evaluations.

语种: 英语
所属项目编号: 30430760
项目资助者: National Science Foundation of China
WOS记录号: WOS:000259165800012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61656
Appears in Collections:北京大学药学院_药剂学系_期刊论文

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作者单位: 1.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100083, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China

Recommended Citation:
Zhang, Yu-Teng,Lu, Wei,Li, Ting,et al. A continued study on the stealth liposomal topotecan plus amlodipine: In vitro and in vivo characterization in non-resistant solid tumors[J]. JOURNAL OF HEALTH SCIENCE,2008,54(4):450-463.
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