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A continued study on the stealth liposomal topotecan plus amlodipine: In vitro and in vivo characterization in non-resistant solid tumors
Zhang, Yu-Teng1; Lu, Wei1,2; Li, Ting1; Liang, Gong-Wen1; Sun, Jia-Bei1; Guo, Jia1; Men, Ying1; Du, Ju1; Lu, Wan-Liang1,2
关键词stealth liposomal topotecan plus amlodipine solid tumors anti-tumor activity murine sarcoma cell line S180 human breast cancer cell line MCF-7
刊名JOURNAL OF HEALTH SCIENCE
2008-08-01
54期:4页:450-463
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Toxicology
资助者National Science Foundation of China ; National Science Foundation of China
研究领域[WOS]Toxicology
关键词[WOS]BREAST-CANCER CELLS ; LONG-CIRCULATING LIPOSOMES ; CARCINOMA A431 CELLS ; CA2+ CHANNEL BLOCKER ; MULTIDRUG-RESISTANCE ; LIPID NANOPARTICLES ; ANTITUMOR EFFICACY ; DRUG-RESISTANCE ; DELIVERY ; DOXORUBICIN
英文摘要

We have previously established a type of anti-resistant stealth liposomal topotecan plus amlodipine for over-coming the multi-drug resistance (MDR) in resistant leukemia cells. The objective of the present study was to further characterize it in the diversified non-resistant solid tumors in vitro and in vivo. Stealth liposomal topotecan plus amlodipine was re-prepared and physicochemically characterized. The in vitro drug release assays of topotecan and amlodipine from liposomes were performed using a dialysis method. 3-(4.5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assays were performed in murine sarcoma S 180 cells and human breast cancer MCF-7 cells, respectively. Apoptotic percentages of S 180 cells were evaluated using flow cytometry. In vitro anti-tumor activity study and terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) analysis were performed in male Institute of Cancer Research (ICR) mice with S180 xenografts. Stealth liposomal topotecan plus amlodipine exhibited high drug encapsulation efficiencies, suitable particle size distribution, negatively charged zeta potential and prolonged release profiles for both topotecan and amlodipine. Amlodipine potentiated the antiproliferative effect and inducing apoptotic effect of topotecan on the tumor cells, exhibiting an additive anti-tumor effect. Stealth liposomal topotecan Plus amlodipine showed the optimal anti-tumor activity and inducing apoptotic effect in the in vivo studies. Stealth liposomal topotecan plus amlodipine demonstrated an overt anti-tumor activity in non-resistant solid tumors, suggesting that it deserves further clinical evaluations.

语种英语
所属项目编号30430760
资助者National Science Foundation of China ; National Science Foundation of China
WOS记录号WOS:000259165800012
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61656
专题北京大学药学院_药剂学系
作者单位1.Peking Univ, Sch Pharmaceut Sci, Dept Pharmaceut, Beijing 100083, Peoples R China
2.Peking Univ, State Key Lab Nat & Biomimet Drugs, Beijing 100083, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Yu-Teng,Lu, Wei,Li, Ting,et al. A continued study on the stealth liposomal topotecan plus amlodipine: In vitro and in vivo characterization in non-resistant solid tumors[J]. JOURNAL OF HEALTH SCIENCE,2008,54(4):450-463.
APA Zhang, Yu-Teng.,Lu, Wei.,Li, Ting.,Liang, Gong-Wen.,Sun, Jia-Bei.,...&Lu, Wan-Liang.(2008).A continued study on the stealth liposomal topotecan plus amlodipine: In vitro and in vivo characterization in non-resistant solid tumors.JOURNAL OF HEALTH SCIENCE,54(4),450-463.
MLA Zhang, Yu-Teng,et al."A continued study on the stealth liposomal topotecan plus amlodipine: In vitro and in vivo characterization in non-resistant solid tumors".JOURNAL OF HEALTH SCIENCE 54.4(2008):450-463.
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