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学科主题口腔医学
Human periodontal ligament cells express osteoblastic phenotypes under intermittent force loading in vitro
Yang, YQ; Li, XT; Rabie, ABM; Fu, MK; Zhang, D
关键词human periodontal ligament cell mechanical force osteoblast
刊名FRONTIERS IN BIOSCIENCE
2006
11页:776-U21
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]KAPPA-B LIGAND ; OSTEOCLAST DIFFERENTIATION ; ALKALINE-PHOSPHATASE ; RECEPTOR ACTIVATOR ; TOOTH MOVEMENT ; OSTEOPROTEGERIN ; FIBROBLASTS ; MODULATION
英文摘要

Mechanical strain applied to bone leads to bone remodeling. In the oral cavity, it is unclear how such mechanical force applied to move teeth orthodontically induces alveolar bone remodeling. It is known that osteoclasts are the only cells that are responsible for bone resorption, while the formation and activity of osteoclasts are regulated by osteoblasts. So it is believed that osteoblasts play an important role not only in bone formation but in bone remodeling as well. Therefore, the purpose of this study was to examine the effect of mechanical force on human periodontal ligament (PDL) cells and whether they express osteoblastic characters in vitro. Methods: Human PDL cells cultured in vitro were loaded with intermittently stretching force for 24 hours. The expression of alkaline phosphatase ( ALP), osteocalcin (OCN) and osteoprotegerin (OPG) were detected at mRNA and protein levels at 0, 2nd, 4th, 6th, 12th, 24th hours after intermittent force loading. Results: Without any stimulation, ALP and OPG mRNA expressions were detected in human PDL cells by in-situ hybridization, but not that of OCN mRNA. ALP mRNA signal was up-regulated and that of OPG was down-regulated by mechanical force within 24 hours. OCN mRNA expression was induced by mechanical force in the late phase of the 24-hours loading cycle. The changes in secreted proteins showed similar results with those seen at the mRNA level. Conclusion: Human PDL cells express osteoblastic phenotypes under intermittent force loading and play a role in alveolar bone remodeling.

语种英语
WOS记录号WOS:000232528000061
引用统计
被引频次:28[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61663
专题北京大学口腔医学院_牙周科
作者单位1.Peking Univ, Sch Stomatol, Dept Orthodont, Beijing 100081, Peoples R China
2.Univ Hong Kong, Fac Dent, Hong Kong, Hong Kong, Peoples R China
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GB/T 7714
Yang, YQ,Li, XT,Rabie, ABM,et al. Human periodontal ligament cells express osteoblastic phenotypes under intermittent force loading in vitro[J]. FRONTIERS IN BIOSCIENCE,2006,11:776-U21.
APA Yang, YQ,Li, XT,Rabie, ABM,Fu, MK,&Zhang, D.(2006).Human periodontal ligament cells express osteoblastic phenotypes under intermittent force loading in vitro.FRONTIERS IN BIOSCIENCE,11,776-U21.
MLA Yang, YQ,et al."Human periodontal ligament cells express osteoblastic phenotypes under intermittent force loading in vitro".FRONTIERS IN BIOSCIENCE 11(2006):776-U21.
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