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学科主题: 口腔医学
题名:
Human periodontal ligament cells express osteoblastic phenotypes under intermittent force loading in vitro
作者: Yang, YQ; Li, XT; Rabie, ABM; Fu, MK; Zhang, D
关键词: human periodontal ligament cell ; mechanical force ; osteoblast
刊名: FRONTIERS IN BIOSCIENCE
发表日期: 2006
卷: 11, 页:776-U21
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Cell Biology
研究领域[WOS]: Biochemistry & Molecular Biology ; Cell Biology
关键词[WOS]: KAPPA-B LIGAND ; OSTEOCLAST DIFFERENTIATION ; ALKALINE-PHOSPHATASE ; RECEPTOR ACTIVATOR ; TOOTH MOVEMENT ; OSTEOPROTEGERIN ; FIBROBLASTS ; MODULATION
英文摘要:

Mechanical strain applied to bone leads to bone remodeling. In the oral cavity, it is unclear how such mechanical force applied to move teeth orthodontically induces alveolar bone remodeling. It is known that osteoclasts are the only cells that are responsible for bone resorption, while the formation and activity of osteoclasts are regulated by osteoblasts. So it is believed that osteoblasts play an important role not only in bone formation but in bone remodeling as well. Therefore, the purpose of this study was to examine the effect of mechanical force on human periodontal ligament (PDL) cells and whether they express osteoblastic characters in vitro. Methods: Human PDL cells cultured in vitro were loaded with intermittently stretching force for 24 hours. The expression of alkaline phosphatase ( ALP), osteocalcin (OCN) and osteoprotegerin (OPG) were detected at mRNA and protein levels at 0, 2nd, 4th, 6th, 12th, 24th hours after intermittent force loading. Results: Without any stimulation, ALP and OPG mRNA expressions were detected in human PDL cells by in-situ hybridization, but not that of OCN mRNA. ALP mRNA signal was up-regulated and that of OPG was down-regulated by mechanical force within 24 hours. OCN mRNA expression was induced by mechanical force in the late phase of the 24-hours loading cycle. The changes in secreted proteins showed similar results with those seen at the mRNA level. Conclusion: Human PDL cells express osteoblastic phenotypes under intermittent force loading and play a role in alveolar bone remodeling.

语种: 英语
WOS记录号: WOS:000232528000061
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61663
Appears in Collections:北京大学口腔医学院_牙周科_期刊论文

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作者单位: 1.Peking Univ, Sch Stomatol, Dept Orthodont, Beijing 100081, Peoples R China
2.Univ Hong Kong, Fac Dent, Hong Kong, Hong Kong, Peoples R China

Recommended Citation:
Yang, YQ,Li, XT,Rabie, ABM,et al. Human periodontal ligament cells express osteoblastic phenotypes under intermittent force loading in vitro[J]. FRONTIERS IN BIOSCIENCE,2006,11:776-U21.
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