北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科  > 期刊论文
学科主题: 临床医学
题名:
Internalization of the TGF-beta type I receptor into caveolin-1 and EEA1 double-positive early endosomes
作者: He, Kangmin1,2,3,4; Yan, Xiaohua5; Li, Nan1; Dang, Song1; Xu, Li1; Zhao, Bing5; Li, Zijian2,3,4; Lv, Zhizhen2,3,4; Fang, Xiaohong1; Zhang, Youyi2,3,4; Chen, Ye-Guang5
关键词: clathrin ; caveolae ; TGF-beta receptor ; caveolin-1-positive early endosomes ; endocytic trafficking
刊名: CELL RESEARCH
发表日期: 2015-06-01
DOI: 10.1038/cr.2015.60
卷: 25, 期:6, 页:738-752
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology
研究领域[WOS]: Cell Biology
关键词[WOS]: CLATHRIN-INDEPENDENT ENDOCYTOSIS ; MEDIATED ENDOCYTOSIS ; COATED PITS ; MEMBRANE ; SMAD2 ; PATHWAY ; CELLS ; PROTEIN ; SARA ; PHOSPHORYLATION
英文摘要:

Endocytosis and intracellular sorting of transforming growth factor-beta (TGF-beta) receptors play an important regulatory role in TGF-beta signaling. Two major endocytic pathways, clathrin-and caveolae-mediated endocytosis, have been reported to independently mediate the internalization of TGF-beta receptors. In this study, we demonstrate that the clathrin-and caveolae-mediated endocytic pathways can converge during TGF-beta receptor endocytic trafficking. By tracking the intracellular dynamics of fluorescently-labeled TGF-beta type I receptor (T beta RI), we found that after mediating T beta RI internalization, certain clathrin-coated vesicles and caveolar vesicles are fused underneath the plasma membrane, forming a novel type of caveolin-1 and clathrin double-positive vesicles. Under the regulation of Rab5, the fused vesicles are targeted to early endosomes and thus deliver the internalized T beta RI to the caveolin-1 and EEA1 double-positive early endosomes (caveolin-1-positive early endosomes). We further showed that the caveolin-1-positive early endosomes are positive for Smad3/SARA, Rab11 and Smad7/Smurf2, and may act as a multifunctional device for TGF-beta signaling and TGF-beta receptor recycling and degradation. Therefore, these findings uncover a novel scenario of endocytosis, the direct fusion of clathrin-coated and caveolae vesicles during TGF-beta receptor endocytic trafficking, which leads to the formation of the multifunctional sorting device, caveolin-1-positive early endosomes, for TGF-beta receptors.

语种: 英语
所属项目编号: 2013CB933701 ; 91413119 ; 91213305 ; 81270159 ; 31221064
项目资助者: National Basic Research Program of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences
WOS记录号: WOS:000355554800010
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61665
Appears in Collections:北京大学第三临床医学院_心血管内科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Chinese Acad Sci, Nanotechnol Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
2.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100191, Peoples R China
3.Peking Univ, Acad Adv Interdisciplinary Studies, Key Lab Cardiovascular Mol Biol & Regulatory Pept, Minist Hlth,Key Lab Mol Cardiovasc Sci,Minist Edu, Beijing 100191, Peoples R China
4.Beijing Key Lab Cardiovasc Receptors Res, Beijing 100191, Peoples R China
5.Tsinghua Univ, Sch Life Sci, Tsinghua Peking Ctr Life Sci, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China

Recommended Citation:
He, Kangmin,Yan, Xiaohua,Li, Nan,et al. Internalization of the TGF-beta type I receptor into caveolin-1 and EEA1 double-positive early endosomes[J]. CELL RESEARCH,2015,25(6):738-752.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[He, Kangmin]'s Articles
[Yan, Xiaohua]'s Articles
[Li, Nan]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[He, Kangmin]‘s Articles
[Yan, Xiaohua]‘s Articles
[Li, Nan]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace