IR@PKUHSC  > 北京大学第三临床医学院  > 心血管内科
学科主题临床医学
Internalization of the TGF-beta type I receptor into caveolin-1 and EEA1 double-positive early endosomes
He, Kangmin1,2,3,4; Yan, Xiaohua5; Li, Nan1; Dang, Song1; Xu, Li1; Zhao, Bing5; Li, Zijian2,3,4; Lv, Zhizhen2,3,4; Fang, Xiaohong1; Zhang, Youyi2,3,4; Chen, Ye-Guang5
关键词clathrin caveolae TGF-beta receptor caveolin-1-positive early endosomes endocytic trafficking
刊名CELL RESEARCH
2015-06-01
DOI10.1038/cr.2015.60
25期:6页:738-752
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology
资助者National Basic Research Program of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; National Basic Research Program of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences
研究领域[WOS]Cell Biology
关键词[WOS]CLATHRIN-INDEPENDENT ENDOCYTOSIS ; MEDIATED ENDOCYTOSIS ; COATED PITS ; MEMBRANE ; SMAD2 ; PATHWAY ; CELLS ; PROTEIN ; SARA ; PHOSPHORYLATION
英文摘要

Endocytosis and intracellular sorting of transforming growth factor-beta (TGF-beta) receptors play an important regulatory role in TGF-beta signaling. Two major endocytic pathways, clathrin-and caveolae-mediated endocytosis, have been reported to independently mediate the internalization of TGF-beta receptors. In this study, we demonstrate that the clathrin-and caveolae-mediated endocytic pathways can converge during TGF-beta receptor endocytic trafficking. By tracking the intracellular dynamics of fluorescently-labeled TGF-beta type I receptor (T beta RI), we found that after mediating T beta RI internalization, certain clathrin-coated vesicles and caveolar vesicles are fused underneath the plasma membrane, forming a novel type of caveolin-1 and clathrin double-positive vesicles. Under the regulation of Rab5, the fused vesicles are targeted to early endosomes and thus deliver the internalized T beta RI to the caveolin-1 and EEA1 double-positive early endosomes (caveolin-1-positive early endosomes). We further showed that the caveolin-1-positive early endosomes are positive for Smad3/SARA, Rab11 and Smad7/Smurf2, and may act as a multifunctional device for TGF-beta signaling and TGF-beta receptor recycling and degradation. Therefore, these findings uncover a novel scenario of endocytosis, the direct fusion of clathrin-coated and caveolae vesicles during TGF-beta receptor endocytic trafficking, which leads to the formation of the multifunctional sorting device, caveolin-1-positive early endosomes, for TGF-beta receptors.

语种英语
所属项目编号2013CB933701 ; 91413119 ; 91213305 ; 81270159 ; 31221064
资助者National Basic Research Program of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences ; National Basic Research Program of China ; National Natural Science Foundation of China ; Chinese Academy of Sciences
WOS记录号WOS:000355554800010
Citation statistics
Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61665
Collection北京大学第三临床医学院_心血管内科
作者单位1.Chinese Acad Sci, Nanotechnol Inst Chem, Key Lab Mol Nanostruct & Nanotechnol, Beijing Natl Lab Mol Sci, Beijing 100190, Peoples R China
2.Peking Univ, Hosp 3, Inst Vasc Med, Beijing 100191, Peoples R China
3.Peking Univ, Acad Adv Interdisciplinary Studies, Key Lab Cardiovascular Mol Biol & Regulatory Pept, Minist Hlth,Key Lab Mol Cardiovasc Sci,Minist Edu, Beijing 100191, Peoples R China
4.Beijing Key Lab Cardiovasc Receptors Res, Beijing 100191, Peoples R China
5.Tsinghua Univ, Sch Life Sci, Tsinghua Peking Ctr Life Sci, State Key Lab Biomembrane & Membrane Biotechnol, Beijing 100084, Peoples R China
Recommended Citation
GB/T 7714
He, Kangmin,Yan, Xiaohua,Li, Nan,et al. Internalization of the TGF-beta type I receptor into caveolin-1 and EEA1 double-positive early endosomes[J]. CELL RESEARCH,2015,25(6):738-752.
APA He, Kangmin.,Yan, Xiaohua.,Li, Nan.,Dang, Song.,Xu, Li.,...&Chen, Ye-Guang.(2015).Internalization of the TGF-beta type I receptor into caveolin-1 and EEA1 double-positive early endosomes.CELL RESEARCH,25(6),738-752.
MLA He, Kangmin,et al."Internalization of the TGF-beta type I receptor into caveolin-1 and EEA1 double-positive early endosomes".CELL RESEARCH 25.6(2015):738-752.
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