IR@PKUHSC  > 北京大学口腔医学院
学科主题口腔医学
Molecular characteristics of homologous salivary adenoid cystic carcinoma cell lines with different lung metastasis ability
Wang, Lin1; Wang, Yixiang2; Bian, Huan1; Pu, Yinfei1; Guo, Chuanbin1
关键词salivary adenoid cystic carcinoma cell line molecular characteristics short tandem repeat
刊名ONCOLOGY REPORTS
2013-07-01
DOI10.3892/or.2013.2460
30期:1页:207-212
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Oncology
关键词[WOS]PROMOTES MIGRATION ; INVASION ; AUTHENTICATION ; CONTAMINATION ; ACTIVATION ; EXPRESSION ; END
英文摘要

Although the homologous salivary adenoid cystic carcinoma (SACC) cell lines SACC-83 and SACC-LM have already been used as SACC models to investigate the underlying mechanisms of metastasis, the molecular features of these SACC cell lines remain unclear. We screened 136 genes related to metastasis in order to investigate the biological and molecular properties of these two cell lines by short tandem repeat (STR) profiling, immunostaining, transwell invasion assay, real-time PCR and western blotting. STR and immunostaining results showed that SACC-83 and SACC-LM are homologous cancer cell lines, derived from adenoepithelial cells and, to date, are not contaminated by each other or other cancer cell lines. Transwell invasion assay results showed that SACC-LM had increased invasion ability compared to SACC-83. 29 of the 136 differentially expressed genes including EREG, S100P, cyclooxygenase (COX)-2, phospho-Akt (p-Akt), matrix metalloproteinase (MMP)-1, MMP-2, MMP-3, MMP-9, MMP-13 and MMP-14 were found following gene screening in SACC-83 and SACC-LM cells. Compared with SACC-83, SACC-LM presents higher expression of COX-2, S100P and lower expression of MMP-2, p-Akt, which could be candidates for identifying the homologous pair cell lines.

语种英语
所属项目编号81172556
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000321077500029
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61684
Collection北京大学口腔医学院
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China
Recommended Citation
GB/T 7714
Wang, Lin,Wang, Yixiang,Bian, Huan,et al. Molecular characteristics of homologous salivary adenoid cystic carcinoma cell lines with different lung metastasis ability[J]. ONCOLOGY REPORTS,2013,30(1):207-212.
APA Wang, Lin,Wang, Yixiang,Bian, Huan,Pu, Yinfei,&Guo, Chuanbin.(2013).Molecular characteristics of homologous salivary adenoid cystic carcinoma cell lines with different lung metastasis ability.ONCOLOGY REPORTS,30(1),207-212.
MLA Wang, Lin,et al."Molecular characteristics of homologous salivary adenoid cystic carcinoma cell lines with different lung metastasis ability".ONCOLOGY REPORTS 30.1(2013):207-212.
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