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学科主题: 口腔医学
题名:
Molecular characteristics of homologous salivary adenoid cystic carcinoma cell lines with different lung metastasis ability
作者: Wang, Lin1; Wang, Yixiang2; Bian, Huan1; Pu, Yinfei1; Guo, Chuanbin1
关键词: salivary adenoid cystic carcinoma cell line ; molecular characteristics ; short tandem repeat
刊名: ONCOLOGY REPORTS
发表日期: 2013-07-01
DOI: 10.3892/or.2013.2460
卷: 30, 期:1, 页:207-212
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: PROMOTES MIGRATION ; INVASION ; AUTHENTICATION ; CONTAMINATION ; ACTIVATION ; EXPRESSION ; END
英文摘要:

Although the homologous salivary adenoid cystic carcinoma (SACC) cell lines SACC-83 and SACC-LM have already been used as SACC models to investigate the underlying mechanisms of metastasis, the molecular features of these SACC cell lines remain unclear. We screened 136 genes related to metastasis in order to investigate the biological and molecular properties of these two cell lines by short tandem repeat (STR) profiling, immunostaining, transwell invasion assay, real-time PCR and western blotting. STR and immunostaining results showed that SACC-83 and SACC-LM are homologous cancer cell lines, derived from adenoepithelial cells and, to date, are not contaminated by each other or other cancer cell lines. Transwell invasion assay results showed that SACC-LM had increased invasion ability compared to SACC-83. 29 of the 136 differentially expressed genes including EREG, S100P, cyclooxygenase (COX)-2, phospho-Akt (p-Akt), matrix metalloproteinase (MMP)-1, MMP-2, MMP-3, MMP-9, MMP-13 and MMP-14 were found following gene screening in SACC-83 and SACC-LM cells. Compared with SACC-83, SACC-LM presents higher expression of COX-2, S100P and lower expression of MMP-2, p-Akt, which could be candidates for identifying the homologous pair cell lines.

语种: 英语
所属项目编号: 81172556
项目资助者: National Natural Science Foundation of China
WOS记录号: WOS:000321077500029
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61684
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China

Recommended Citation:
Wang, Lin,Wang, Yixiang,Bian, Huan,et al. Molecular characteristics of homologous salivary adenoid cystic carcinoma cell lines with different lung metastasis ability[J]. ONCOLOGY REPORTS,2013,30(1):207-212.
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