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学科主题: 临床医学
题名:
Infection of Mongolian gerbils with Chinese Helicobacter pylori strains
作者: Wang, JT; Court, M; Jeremy, AHT; Aboshkiwa, MA; Robinson, PA; Crabtree, JE
关键词: Helicobacter pylori ; Mongolian gerbil ; gastritis ; Helicobacter pylori vaccine ; vacA allelic variation
刊名: FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
发表日期: 2003-05-25
DOI: 10.1016/S0928-8244(02)00464-9
卷: 36, 期:3, 页:207-213
收录类别: ISTP ; SCI
文章类型: Proceedings Paper
WOS标题词: Science & Technology
类目[WOS]: Immunology ; Infectious Diseases ; Microbiology
研究领域[WOS]: Immunology ; Infectious Diseases ; Microbiology
关键词[WOS]: INFLAMMATORY RESPONSES ; GASTRIC-CANCER ; HOST ; CAGA ; MICE ; CYTOTOXIN ; DISEASE ; MODEL ; CELLS ; VACA
英文摘要:

To date only a few Helicobacter pylori strains have been demonstrated to colonise Mongolian gerbils successfully. The aim of this study was to establish stable colonisation of Chinese strains of H. pylori in gerbils. Fresh clinical isolates from Chinese patients were inoculated into gerbils. At 4-6 weeks post inoculation, infection status was evaluated by culture, biopsy urease test and pathology. Sequencing of glmM and random amplified polymorphic DNA (RAPD) fingerprinting of DNA from cultured H. pylori were used to evaluate the genetic identity of pre-inoculated and post-inoculated strains. The ability of pre- and post-inoculated strains to stimulate interleukin-8 transcription in L5F11 gastric epithelial cells was analysed. Three of five clinical isolates colonised gerbils. The three pre- and post-inoculation strains had identical glmM sequences and RAPD profiles, and stimulated luciferase secretion from L5F11 epithelial cells. The strain that caused severe pathological changes was selected for repeat infection to prove reproducible and stable colonisation. The cagA+ strain 42GX gave stable colonisation in the gerbil and induced severe gastritis. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

语种: 英语
WOS记录号: WOS:000182940400015
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61688
Appears in Collections:北京大学第二临床医学院_消化内科_期刊论文

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作者单位: 1.St James Univ Hosp, Mol Med Unit, Leeds LS9 7TF, W Yorkshire, England
2.Beijing Univ, Dept Gastroenterol, Peoples Hosp, Beijing, Peoples R China

Recommended Citation:
Wang, JT,Court, M,Jeremy, AHT,et al. Infection of Mongolian gerbils with Chinese Helicobacter pylori strains[J]. FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY,2003,36(3):207-213.
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