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Infection of Mongolian gerbils with Chinese Helicobacter pylori strains
Wang, JT; Court, M; Jeremy, AHT; Aboshkiwa, MA; Robinson, PA; Crabtree, JE
关键词Helicobacter pylori Mongolian gerbil gastritis Helicobacter pylori vaccine vacA allelic variation
刊名FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY
2003-05-25
DOI10.1016/S0928-8244(02)00464-9
36期:3页:207-213
收录类别ISTP ; SCI
文章类型Proceedings Paper
WOS标题词Science & Technology
类目[WOS]Immunology ; Infectious Diseases ; Microbiology
研究领域[WOS]Immunology ; Infectious Diseases ; Microbiology
关键词[WOS]INFLAMMATORY RESPONSES ; GASTRIC-CANCER ; HOST ; CAGA ; MICE ; CYTOTOXIN ; DISEASE ; MODEL ; CELLS ; VACA
英文摘要

To date only a few Helicobacter pylori strains have been demonstrated to colonise Mongolian gerbils successfully. The aim of this study was to establish stable colonisation of Chinese strains of H. pylori in gerbils. Fresh clinical isolates from Chinese patients were inoculated into gerbils. At 4-6 weeks post inoculation, infection status was evaluated by culture, biopsy urease test and pathology. Sequencing of glmM and random amplified polymorphic DNA (RAPD) fingerprinting of DNA from cultured H. pylori were used to evaluate the genetic identity of pre-inoculated and post-inoculated strains. The ability of pre- and post-inoculated strains to stimulate interleukin-8 transcription in L5F11 gastric epithelial cells was analysed. Three of five clinical isolates colonised gerbils. The three pre- and post-inoculation strains had identical glmM sequences and RAPD profiles, and stimulated luciferase secretion from L5F11 epithelial cells. The strain that caused severe pathological changes was selected for repeat infection to prove reproducible and stable colonisation. The cagA+ strain 42GX gave stable colonisation in the gerbil and induced severe gastritis. (C) 2003 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

语种英语
WOS记录号WOS:000182940400015
引用统计
被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61688
专题北京大学第二临床医学院_消化科
作者单位1.St James Univ Hosp, Mol Med Unit, Leeds LS9 7TF, W Yorkshire, England
2.Beijing Univ, Dept Gastroenterol, Peoples Hosp, Beijing, Peoples R China
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GB/T 7714
Wang, JT,Court, M,Jeremy, AHT,et al. Infection of Mongolian gerbils with Chinese Helicobacter pylori strains[J]. FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY,2003,36(3):207-213.
APA Wang, JT,Court, M,Jeremy, AHT,Aboshkiwa, MA,Robinson, PA,&Crabtree, JE.(2003).Infection of Mongolian gerbils with Chinese Helicobacter pylori strains.FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY,36(3),207-213.
MLA Wang, JT,et al."Infection of Mongolian gerbils with Chinese Helicobacter pylori strains".FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY 36.3(2003):207-213.
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