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学科主题: 药学
题名:
Comprehensive chemical analysis of triterpenoids and polysaccharides in the medicinal mushroom Antrodia cinnamomea
作者: Qiao, Xue1; Song, Wei1; Wang, Qi1; Liu, Ke-di1; Zhang, Zheng-xiang3; Bo, Tao3; Li, Ren-yong4; Liang, Li-na4; Tzeng, Yew-min5; Guo, De-an1,2; Ye, Min1,2
刊名: RSC ADVANCES
发表日期: 2015
DOI: 10.1039/c5ra04327a
卷: 5, 期:58, 页:47040-47052
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Chemistry, Multidisciplinary
研究领域[WOS]: Chemistry
关键词[WOS]: ANION-EXCHANGE CHROMATOGRAPHY ; PULSED AMPEROMETRIC DETECTION ; MASS-SPECTROMETRY ; GANODERMA-LUCIDUM ; FRUITING BODIES ; CAMPHORATA ; ERGOSTANE ; SEPARATION ; ACID
英文摘要:

Antrodia cinnamomea is a precious medicinal mushroom used in adjuvant treatments of cancer. Triterpenoids (25R/S-ergostane epimers and Delta(7,9)/Delta(8)-lanostanes) and polysaccharides are its major bioactive constituents. Quality control of this mushroom is difficult, due to a lack of effective analytical methods. In this study, a series of methods were established to analyze A. cinnamomea. The ergostane- and lanostane-type triterpenoids were extracted by 50% and 100% methanol, respectively, and were then analyzed by ultra-high performance liquid chromatography coupled with diode-array detection and quadrupole time-of-flight mass spectrometry (UHPLC/DAD/qTOF-MS). In total 49 compounds, including 34 ergostanes and 10 lanostanes, were characterized by comparing with 31 reference standards or by analyzing the UV and MS spectral data. Furthermore, the contents of 18 major triterpenoids, including 10 ergostanes and 8 lanostanes, were determined by UPLC/UV or supercritical fluid chromatography coupled with mass spectrometry (SFC/MS, for 25R/S-antcin A) within 16 min. This is the first time that the contents of pure optical forms for 5 pairs of 25R- and 25S-ergostane epimers (antcins A, B, C, H, and K) were determined. On the other hand, the polysaccharides in A. cinnamomea were analyzed by ion chromatography coupled with pulsed amperometric detection (IC/PAD) after acid hydrolysis, and the contents of 7 monosaccharides were determined. Finally, the established methods were applied to the analysis of 15 batches of A. cinnamomea. Samples derived from different cultivation techniques could be distinguished according to the contents of triterpenoids. The UPLC/UV, SFC/MS and IC/PAD methods established in this work provided powerful tools to evaluate the quality of A. cinnamomea.

语种: 英语
所属项目编号: 81222054 ; 81303294 ; NCET-11-0019 ; SIMM1403KF-16
项目资助者: National Natural Science Foundation of China ; Program for New Century Excellent Talents in University from Chinese Ministry of Education ; State Key Laboratory of Drug Research
WOS记录号: WOS:000355702900075
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61692
Appears in Collections:北京大学药学院_期刊论文

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作者单位: 1.Agilent Technol, Beijing 100102, Peoples R China
2.ThermoFisher Sci Ltd, Beijing 100085, Peoples R China
3.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, Beijing 100191, Peoples R China
4.Chinese Acad Sci, Shanghai Inst Mat Med, State Key Lab Drug Res, Shanghai 201203, Peoples R China
5.Chaoyang Univ Technol, Inst Biochem Sci & Technol, Taichung 41349, Taiwan

Recommended Citation:
Qiao, Xue,Song, Wei,Wang, Qi,et al. Comprehensive chemical analysis of triterpenoids and polysaccharides in the medicinal mushroom Antrodia cinnamomea[J]. RSC ADVANCES,2015,5(58):47040-47052.
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