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学科主题: 公共卫生
题名:
Automobile exhaust particle-induced apoptosis and necrosis in MRC-5 cells
作者: Zhao, XH; Wang, XL; Li, XY
关键词: automobile exhaust pollution ; particle extracts (PE) ; MRC-5 cell ; apoptosis ; necrosis
刊名: TOXICOLOGY LETTERS
发表日期: 2001-05-31
卷: 122, 期:1, 页:103-110
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Toxicology
研究领域[WOS]: Toxicology
英文摘要:

To study the effect of particulate extracts (PE) collected from a heavy traffic road in Lanzhou City, on MRC-5 cell apoptosis, and to explore the toxicity action of PE and its mechanism. Cultured MRC-5 cells were incubated in the extracts of different concentrations. Inhibition of proliferation was measured with a colorimetric 3-[4,5-dimethyl thiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Morphological assessment of apoptosis was performed with fluorescence microscopy and electronic microscopy. Extracted DNA from the cells was electrophoresed on agarose gel in order to observe DNA fragmentation. The amount of apoptotic cells was measured by flow cytometry. The results indicated that exposure of exponentially growing MRC-5 cells exposed to PE 5-160 mug 1(-1) for 24-96 h resulted in dose- and time-dependent reduction of survival of MRC-5 cells. After treatment with PE, markedly morphological changes of MRC-5 cells including ′apoptotic bodies, were observed with a fluorescence microscope. Agarose gel electrophoresis of DNA from the cells treated with PE for 48 and 72 h revealed a ′ladder′ pattern. PE induced apoptosis in low doses but necrosis in high doses. Apoptotic rates were 12.95, 17.40 and 29.80% after treatment with PE 5, 10, and 20 mug 1(-1), respectively. A typical sub-diploid apoptosis peak was demonstrated in MRC-5 cells treated with PE. A significant dose-effect response and time-effect correlation could be found between apoptosis rates and PE. All results confirmed that the PE could induce and accelerate apoptosis in low doses but necrosis in high does. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

语种: 英语
WOS记录号: WOS:000169541200011
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61694
Appears in Collections:北京大学公共卫生学院_期刊论文

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作者单位: 1.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China
2.Peking Univ, Sch Publ Hlth, Dept Environm & Occupat Hlth, Beijing 100083, Peoples R China
3.Lanzhou Med Coll, Inst Haematol, Lanzhou 730000, Peoples R China

Recommended Citation:
Zhao, XH,Wang, XL,Li, XY. Automobile exhaust particle-induced apoptosis and necrosis in MRC-5 cells[J]. TOXICOLOGY LETTERS,2001,122(1):103-110.
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