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Automobile exhaust particle-induced apoptosis and necrosis in MRC-5 cells
Zhao, XH; Wang, XL; Li, XY
关键词automobile exhaust pollution particle extracts (PE) MRC-5 cell apoptosis necrosis
刊名TOXICOLOGY LETTERS
2001-05-31
122期:1页:103-110
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Toxicology
研究领域[WOS]Toxicology
英文摘要

To study the effect of particulate extracts (PE) collected from a heavy traffic road in Lanzhou City, on MRC-5 cell apoptosis, and to explore the toxicity action of PE and its mechanism. Cultured MRC-5 cells were incubated in the extracts of different concentrations. Inhibition of proliferation was measured with a colorimetric 3-[4,5-dimethyl thiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Morphological assessment of apoptosis was performed with fluorescence microscopy and electronic microscopy. Extracted DNA from the cells was electrophoresed on agarose gel in order to observe DNA fragmentation. The amount of apoptotic cells was measured by flow cytometry. The results indicated that exposure of exponentially growing MRC-5 cells exposed to PE 5-160 mug 1(-1) for 24-96 h resulted in dose- and time-dependent reduction of survival of MRC-5 cells. After treatment with PE, markedly morphological changes of MRC-5 cells including ′apoptotic bodies, were observed with a fluorescence microscope. Agarose gel electrophoresis of DNA from the cells treated with PE for 48 and 72 h revealed a ′ladder′ pattern. PE induced apoptosis in low doses but necrosis in high doses. Apoptotic rates were 12.95, 17.40 and 29.80% after treatment with PE 5, 10, and 20 mug 1(-1), respectively. A typical sub-diploid apoptosis peak was demonstrated in MRC-5 cells treated with PE. A significant dose-effect response and time-effect correlation could be found between apoptosis rates and PE. All results confirmed that the PE could induce and accelerate apoptosis in low doses but necrosis in high does. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.

语种英语
WOS记录号WOS:000169541200011
引用统计
被引频次:2[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61694
专题北京大学公共卫生学院
作者单位1.Lanzhou Univ, Sch Life Sci, Lanzhou 730000, Peoples R China
2.Peking Univ, Sch Publ Hlth, Dept Environm & Occupat Hlth, Beijing 100083, Peoples R China
3.Lanzhou Med Coll, Inst Haematol, Lanzhou 730000, Peoples R China
推荐引用方式
GB/T 7714
Zhao, XH,Wang, XL,Li, XY. Automobile exhaust particle-induced apoptosis and necrosis in MRC-5 cells[J]. TOXICOLOGY LETTERS,2001,122(1):103-110.
APA Zhao, XH,Wang, XL,&Li, XY.(2001).Automobile exhaust particle-induced apoptosis and necrosis in MRC-5 cells.TOXICOLOGY LETTERS,122(1),103-110.
MLA Zhao, XH,et al."Automobile exhaust particle-induced apoptosis and necrosis in MRC-5 cells".TOXICOLOGY LETTERS 122.1(2001):103-110.
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