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学科主题: 口腔医学
题名:
Activation of transient receptor potential vanilloid subtype 1 increases secretion of the hypofunctional, transplanted submandibular gland
作者: Zhang, Y.1,2; Cong, X.1,2; Shi, L.3,4,5; Xiang, B.3,4,6; Li, Y. M.1,2,7; Ding, Q. W.3,4; Ding, C.1,2; Wu, L. L.1,2; Yu, G. Y.3,4
关键词: capsaicin ; aquaporin 5 ; transient receptor potential vanilloid subtype 1 ; secretory mechanism
刊名: AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY
发表日期: 2010-07-01
DOI: 10.1152/ajpgi.00528.2009
卷: 299, 期:1, 页:G54-G62
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology ; Physiology
研究领域[WOS]: Gastroenterology & Hepatology ; Physiology
关键词[WOS]: KERATOCONJUNCTIVITIS SICCA ; PROTEIN-KINASE ; AQUAPORIN-5 EXPRESSION ; SJOGRENS-SYNDROME ; EPITHELIAL-CELLS ; CAPSAICIN CREAM ; PATHWAY ; CHANNEL ; TRAFFICKING ; MECHANISMS
英文摘要:

Zhang Y, Cong X, Shi L, Ding QW, Ding C, Wu LL, Yu GY. Activation of transient receptor potential vanilloid subtype 1 increases secretion of the hypofunctional, transplanted submandibular gland. Am J Physiol Gastrointest Liver Physiol 299: G54-G62, 2010. First published April 1, 2010; doi:10.1152/ajpgi.00528.2009.-Hyposecretion occurs in most patients early after submandibular gland autotransplantation for severe keratoconjunctivitis sicca. Endogenous transient receptor potential vanilloid subtype 1 (TRPV1) has been recently demonstrated in rabbit submandibular glands, and activation of TRPV1 by capsaicin increases secretion in isolated glands, but the TRPV1-mediated secretory mechanism remains to be elucidated. The purpose of this study was to verify whether activation of TRPV1 by capsaicin could improve the secretion of transplanted gland and its underlying mechanism. The salivary flow of the transplanted glands was significantly decreased, and the mRNA and protein levels of TRPV1 and aquaporin 5 (AQP5) were downregulated in the transplanted glands. Topical capsaicin cream increased secretion and upregulated levels of TRPV1 and AQP5 in transplanted glands. Moreover, in cultured submandibular gland cells, capsaicin increased the mRNA expression of AQP5 and led to redistribution of AQP5 from the cytoplasm to the plasma membrane via TRPV1 activation. Capsaicin enhanced the phosphorylation of extracellular signal-regulated kinase (ERK). Preincubation of cells with PD98059, an inhibitor of ERK kinase, suppressed the capsaicin-induced mRNA expression of AQP5. In summary, the capsaicin-induced secretory mechanism involved activation of TRPV1 and upregulation of AQP5 in an ERK-dependent manner and promoted the redistribution of AQP5 in submandibular gland cells. Activation of TRPV1 may provide a new therapeutic strategy to improve submandibular gland hypofunction.

语种: 英语
所属项目编号: 30700949 ; 30730102 ; 2007BAI18B11 ; 2005-2009
项目资助者: National Nature Science Foundation of China ; National Supporting Program for Science and Technology ; Capital Medical Development Foundation
WOS记录号: WOS:000278792600005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61704
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Hlth Sci Ctr, Dept Physiol & Pathophysiol, Beijing 100191, Peoples R China
2.Minist Educ, Key Lab Mol Cardiovasc Sci, Beijing, Peoples R China
3.Dalian Univ, Coll Med, Dept Oral Med, Dalian, Peoples R China
4.Peking Univ, Dept Oral & Maxillofacial Surg, Sch Stomatol, Beijing 100871, Peoples R China
5.Peking Univ, Dept Oral & Maxillofacial Surg, Hosp Stomatol, Beijing 100871, Peoples R China
6.Shandong Univ, Qilu Hosp, Dept Oral & Maxillofacial Surg, Jinan, Shandong, Peoples R China
7.Capital Med Univ, Yanjing Med Coll, Dept Physiol & Pathophysiol, Beijing, Peoples R China

Recommended Citation:
Zhang, Y.,Cong, X.,Shi, L.,et al. Activation of transient receptor potential vanilloid subtype 1 increases secretion of the hypofunctional, transplanted submandibular gland[J]. AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY,2010,299(1):G54-G62.
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