北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 免疫学系  > 期刊论文
学科主题: 基础医学
题名:
Identification of five human novel genes associated with cell proliferation by cell-based screening from an expressed cDNA ORF library
作者: Ma, Xi; Wang, Xinyu; Gao, Xia; Wang, Lan; Lu, Yang; Gao, Peng; Deng, Weiwei; Yu, Peng; Ma, Jinjing; Guo, Jinhai; Cheng, Hualing; Zhang, Chenying; Shi, Taiping; Ma, Dalong
关键词: high-throughput screening ; cell proliferation ; reporter gene ; functional genomics
刊名: LIFE SCIENCES
发表日期: 2007-09-15
DOI: 10.1016/j.1fs.2007.08.006
卷: 81, 期:14, 页:1141-1151
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Medicine, Research & Experimental ; Pharmacology & Pharmacy
研究领域[WOS]: Research & Experimental Medicine ; Pharmacology & Pharmacy
关键词[WOS]: FLOW-CYTOMETRY ; ASSAY ; DATABASE ; PROTEIN ; KINASE ; GROWTH ; CANCER ; DYES
英文摘要:

The development of functional profiling technologies provides opportunity for high-throughput functional genomics studies. We describe a cell-based screening system to identify novel human genes associated with cell proliferation. The method integrates luciferase reporter gene activity, fluorescence stain, automated microscopy and cellular phenotype assays. We successfully used the system to screen 409 novel human genes cloned by our lab and found that 27 genes significantly up-regulated promoter-Renilla luciferase reporter plasmid (pRL) activity. Among them, five genes, TRAF3IP3, ZNF306, ZNF250, SGOL1, and ZNF434, were determined through morphological observation, calcein AM fluorescence stain, MTT assay and cell cycle analysis to be associated with cell proliferation. Furthermore, we showed that the gene TRAF3IP3, which initially was identified to specifically interact with TRAF3, stimulated cell growth by modulating the c-Jun N-terminal kinase (INK) pathway, and RNAi of TRAF3IP3 confirmed that the effect was physiological and necessary. In summary, we integrated a rapid and efficient system for screening novel growth regulatory genes. Using the new screening system we identified five genes associated with cell proliferation for the first time. (C) 2007 Elsevier Inc. All rights reserved.

语种: 英语
WOS记录号: WOS:000250160000004
Citation statistics:
内容类型: 期刊论文
版本: 出版稿
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61713
Appears in Collections:基础医学院_免疫学系_期刊论文

Files in This Item:
File Name/ File Size Content Type Version Access License
1-s2.0-S0024320507005851-main.pdf(1551KB)期刊论文出版稿限制开放 联系获取全文

作者单位: 1.Chinese Natl Human Genome Ctr, Beijing 100176, Peoples R China
2.Peking Univ, Hlth Sci Ctr, Lab Med Immunol, Sch Basic Med Sci, Beijing 100083, Peoples R China
3.Peking Univ, Human Dis Genom Ctr, Beijing 100083, Peoples R China

Recommended Citation:
Ma, Xi,Wang, Xinyu,Gao, Xia,et al. Identification of five human novel genes associated with cell proliferation by cell-based screening from an expressed cDNA ORF library[J]. LIFE SCIENCES,2007,81(14):1141-1151.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Ma, Xi]'s Articles
[Wang, Xinyu]'s Articles
[Gao, Xia]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Ma, Xi]‘s Articles
[Wang, Xinyu]‘s Articles
[Gao, Xia]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace