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学科主题: 临床医学
题名:
TSLP Expression Induced via Toll-Like Receptor Pathways in Human Keratinocytes
作者: Takai, Toshiro1; Chen, Xue1,2; Xie, Yang1,3; Anh Tuan Vu1,4,5; Tuan Anh Le1,4,6; Kinoshita, Hirokazu1,4; Kawasaki, Junko1,4; Kamijo, Seiji1; Hara, Mutsuko1; Ushio, Hiroko1; Baba, Tadashi7; Hiramatsu, Keiichi7; Ikeda, Shigaku1,4; Ogawa, Hideoki1,4; Okumura, Ko1; Conn, PM
刊名: ENDOSOME SIGNALING, PT B
发表日期: 2014
DOI: 10.1016/B978-0-12-397925-4.00021-3
卷: 535, 页:371-387
收录类别: BSCI ; SCI
文章类型: Book Chapter
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: THYMIC STROMAL LYMPHOPOIETIN ; PATTERN-RECOGNITION RECEPTORS ; INNATE IMMUNE-RESPONSE ; NF-KAPPA-B ; INFLAMMATION ; FLAGELLIN ; GLUCOCORTICOIDS ; INDUCTION ; CYTOKINES ; RELEASE
英文摘要:

The skin epidermis and mucosal epithelia (airway, ocular tissues, gut, and so on) are located at the interface between the body and environment and have critical roles in the response to various stimuli. Thymic stromal lymphopoietin (TSLP), a cytokine expressed mainly by epidermal keratinocytes (KCs) and mucosa] epithelial cells, is a critical factor linking the innate response at barrier surfaces to Th2-skewed acquired immune response. TSLP is highly expressed in skin lesions of atopic dermatitis patients. Here, we describe on Toll-like receptor (TLR)-mediated induction of TSLP expression in primary cultured human KCs, placing emphasis on experimental methods used in our studies. Double-stranded RNA (TLR3 ligand), flagellin (TLR5 ligand), and diacylated lipopeptide (TLR2-TLR6 ligand) stimulated human KCs to express TSLP and Staphylococcus aureus membranes did so via the TLR2-TLR6 pathway. Atopic cytokine milieu upregulated the TLR-mediated induction of TSLP. Culturing in the absence of glucocorticoid before stimulation enhanced the TSLP expression. Extracellular double-stranded RNA induced TSLP via endosomal acidification- and NF-kappa B-dependent pathway. Specific measurement of the long TSLP transcript, which contributes to the production of the TSLP protein, rather than total or the short transcript is useful for accurate detection of functional human TSLP gene expression. The results suggest that environment-, infection-, and/or self-derived TLR ligands contribute to the initiation and/or amplification of Th2-type skin inflammation including atopic dermatitis and atopic march through the induction of TSLP expression in KCs and include information helpful for understanding the role of the gene environment interaction relevant in allergic diseases.

语种: 英语
WOS记录号: WOS:000331018400021
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61717
Appears in Collections:北京大学第二临床医学院_皮科_期刊论文

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作者单位: 1.Juntendo Univ, Grad Sch Med, Atopy Allergy Res Ctr, Tokyo, Japan
2.Peking Univ, Peoples Hosp, Dept Dermatol, Beijing 100871, Peoples R China
3.Sun Yat Sen Univ, Affiliated Hosp 3, Dept Dermatol, Guangzhou 510275, Guangdong, Peoples R China
4.Juntendo Univ, Grad Sch Med, Dept Dermatol & Allergol, Tokyo, Japan
5.Quyhoa Natl Leprosy Dermatol Hosp, Quynhon, Vietnam
6.Inst Clin Med & Pharmaceut Sci 108, Dept Dermatol & Allergol, Hanoi, Vietnam
7.Juntendo Univ, Grad Sch Med, Dept Microbiol & Infect Control Sci, Tokyo, Japan

Recommended Citation:
Takai, Toshiro,Chen, Xue,Xie, Yang,et al. TSLP Expression Induced via Toll-Like Receptor Pathways in Human Keratinocytes[J]. ENDOSOME SIGNALING, PT B,2014,535:371-387.
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