北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 基础医学院  > 病原生物学系  > 期刊论文
学科主题: 基础医学
题名:
Quantitative determination of HER2 expression by confocal microscopy assay in CTCs of breast cancer
作者: Cao, Sheng1,2; Li, Yang1,3; Li, Jin4,5; Li, Chang-Fei1; Zhang, Wei6; Yang, Zeng-Qi2; Meng, Song-Dong1
关键词: HER2 ; overexpression ; confocal microscopy assay ; circulating tumor cells ; quantitative measurement
刊名: ONCOLOGY REPORTS
发表日期: 2010-02-01
DOI: 10.3892/or_00000651
卷: 23, 期:2, 页:423-428
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Oncology
研究领域[WOS]: Oncology
关键词[WOS]: CIRCULATING TUMOR-CELLS ; RESISTANT PROSTATE-CANCER ; POLYMERASE-CHAIN-REACTION ; BONE-MARROW ; PERIPHERAL-BLOOD ; GENE STATUS ; IMMUNOFLUORESCENCE ; THERAPY
英文摘要:

HER2 analysis in circulating tumor cells (CTCs) may have clinical significance for HER2-targeted therapy as HER2-positive CTCs and disseminated tumor cells can be detected in patients with HER2-negative primary tumors who currently do not have access to HER2-targeted therapy. In this study, we performed quantitative analysis by confocal microscopy assay for evaluation of HER2 expression in individual tumor cells. HER2 testing by confocal microscopy assay exhibited high concordance with results of real-time PCR, Western blot analysis and FISH analysis. We found that there was a significant positive correlation between HER2 overexpression and gene amplification in individual CTCs, which provided validation of confocal microscopy assay for HER2 expression in CTCs. By using subsets of 10 consecutive cells (bins), we conclude that HER2 expression (3+) in CTCs predicts HER2 overexpression of tumor with high probability in breast cancer patients. These results may provide interpretive guidelines for HER2 status assay in CTCs and raise great opportunities for using CTCs as non-invasive and ′real-time′ biopsy to examine and monitor the status of tumor markers.

语种: 英语
所属项目编号: 2007CB512802 ; 2007DFC30240
项目资助者: Major State Basic Research Development Program of China (973 Program) ; International Science and Technology Cooperation Project
WOS记录号: WOS:000273873500016
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61733
Appears in Collections:基础医学院_病原生物学系_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Beijing Tiantan Biol Prod Co Ltd, Beijing 100024, Peoples R China
2.Chinese Acad Sci, Inst Microbiol, Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China
3.NW A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China
4.Sichuan Normal Univ, Coll Life Sci, Chengdu 610066, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100191, Peoples R China
6.Chinese Acad Med Sci, Canc Inst & Hosp, Beijing 100021, Peoples R China

Recommended Citation:
Cao, Sheng,Li, Yang,Li, Jin,et al. Quantitative determination of HER2 expression by confocal microscopy assay in CTCs of breast cancer[J]. ONCOLOGY REPORTS,2010,23(2):423-428.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Cao, Sheng]'s Articles
[Li, Yang]'s Articles
[Li, Jin]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Cao, Sheng]‘s Articles
[Li, Yang]‘s Articles
[Li, Jin]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace