IR@PKUHSC  > 北京大学基础医学院  > 病原生物学系
学科主题基础医学
Quantitative determination of HER2 expression by confocal microscopy assay in CTCs of breast cancer
Cao, Sheng1,2; Li, Yang1,3; Li, Jin4,5; Li, Chang-Fei1; Zhang, Wei6; Yang, Zeng-Qi2; Meng, Song-Dong1
关键词HER2 overexpression confocal microscopy assay circulating tumor cells quantitative measurement
刊名ONCOLOGY REPORTS
2010-02-01
DOI10.3892/or_00000651
23期:2页:423-428
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
研究领域[WOS]Oncology
关键词[WOS]CIRCULATING TUMOR-CELLS ; RESISTANT PROSTATE-CANCER ; POLYMERASE-CHAIN-REACTION ; BONE-MARROW ; PERIPHERAL-BLOOD ; GENE STATUS ; IMMUNOFLUORESCENCE ; THERAPY
英文摘要

HER2 analysis in circulating tumor cells (CTCs) may have clinical significance for HER2-targeted therapy as HER2-positive CTCs and disseminated tumor cells can be detected in patients with HER2-negative primary tumors who currently do not have access to HER2-targeted therapy. In this study, we performed quantitative analysis by confocal microscopy assay for evaluation of HER2 expression in individual tumor cells. HER2 testing by confocal microscopy assay exhibited high concordance with results of real-time PCR, Western blot analysis and FISH analysis. We found that there was a significant positive correlation between HER2 overexpression and gene amplification in individual CTCs, which provided validation of confocal microscopy assay for HER2 expression in CTCs. By using subsets of 10 consecutive cells (bins), we conclude that HER2 expression (3+) in CTCs predicts HER2 overexpression of tumor with high probability in breast cancer patients. These results may provide interpretive guidelines for HER2 status assay in CTCs and raise great opportunities for using CTCs as non-invasive and ′real-time′ biopsy to examine and monitor the status of tumor markers.

语种英语
WOS记录号WOS:000273873500016
项目编号2007CB512802 ; 2007DFC30240
资助机构Major State Basic Research Development Program of China (973 Program) ; International Science and Technology Cooperation Project
引用统计
被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61733
专题北京大学基础医学院_病原生物学系
作者单位1.Beijing Tiantan Biol Prod Co Ltd, Beijing 100024, Peoples R China
2.Chinese Acad Sci, Inst Microbiol, Key Lab Pathogen Microbiol & Immunol, Beijing 100101, Peoples R China
3.NW A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China
4.Sichuan Normal Univ, Coll Life Sci, Chengdu 610066, Peoples R China
5.Peking Univ, Hlth Sci Ctr, Dept Microbiol, Beijing 100191, Peoples R China
6.Chinese Acad Med Sci, Canc Inst & Hosp, Beijing 100021, Peoples R China
推荐引用方式
GB/T 7714
Cao, Sheng,Li, Yang,Li, Jin,et al. Quantitative determination of HER2 expression by confocal microscopy assay in CTCs of breast cancer[J]. ONCOLOGY REPORTS,2010,23(2):423-428.
APA Cao, Sheng.,Li, Yang.,Li, Jin.,Li, Chang-Fei.,Zhang, Wei.,...&Meng, Song-Dong.(2010).Quantitative determination of HER2 expression by confocal microscopy assay in CTCs of breast cancer.ONCOLOGY REPORTS,23(2),423-428.
MLA Cao, Sheng,et al."Quantitative determination of HER2 expression by confocal microscopy assay in CTCs of breast cancer".ONCOLOGY REPORTS 23.2(2010):423-428.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Cao, Sheng]的文章
[Li, Yang]的文章
[Li, Jin]的文章
百度学术
百度学术中相似的文章
[Cao, Sheng]的文章
[Li, Yang]的文章
[Li, Jin]的文章
必应学术
必应学术中相似的文章
[Cao, Sheng]的文章
[Li, Yang]的文章
[Li, Jin]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。