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学科主题: 口腔医学
题名:
Preliminary X-ray crystallographic analysis of SMU.2055 protein from the caries pathogen Streptococcus mutans
作者: Zhao, Wang-Hong2,3; Zhan, Xiu-Rong3; Gao, Xiong-Zhuo1; Liu, Xiang1; Zhang, Yi-Fei2; Lin, Jiuxiang2; Li, Lan-Fen1; Wei, Shi-Cheng1; Su, Xio-Dong1
刊名: ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
发表日期: 2010-05-01
DOI: 10.1107/S1744309110010365
卷: 66, 页:530-533
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics ; Crystallography
关键词[WOS]: CRYSTALS
英文摘要:

The SMU.2055 gene from the major caries pathogen Streptococcus mutans is annotated as a putative acetyltransferase with 163 amino-acid residues. In order to identify its function via structural studies, the SMU.2055 gene was cloned into the expression vector pET28a. Native and SeMet-labelled SMU.2055 proteins with a His(6) tag at the N-terminus were expressed at a high level in Escherichia coli strain BL21 (DE3) and purified to homogeneity by Ni(2+)-chelating affinity chromatography. Diffraction-quality crystals of SeMet-labelled SMU.2055 were obtained using the sitting-drop vapour-diffusion method and diffracted to a resolution of 2.5 angstrom on beamline BL17A at the Photon Factory, Tsukuba, Japan. The crystals belong to the orthorhombic space group C222(1), with unit-cell parameters a = 92.0, b = 95.0, c = 192.2 angstrom. The asymmetric unit contained four molecules, with a solvent content of 57.1%.

语种: 英语
所属项目编号: 30530190 ; 985 ; 211
项目资助者: National Natural Science Foundation of China ; Peking University
WOS记录号: WOS:000277217700012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61756
Appears in Collections:北京大学口腔医学院_期刊论文

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作者单位: 1.Peking Univ, Coll Life Sci, Natl Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China
2.Peking Univ, Sch Stomatol, Beijing 100081, Peoples R China
3.Lanzhou Univ, Sch Stomatol, Lanzhou 730000, Peoples R China

Recommended Citation:
Zhao, Wang-Hong,Zhan, Xiu-Rong,Gao, Xiong-Zhuo,et al. Preliminary X-ray crystallographic analysis of SMU.2055 protein from the caries pathogen Streptococcus mutans[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,2010,66:530-533.
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