北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学口腔医学院  > 期刊论文
学科主题: 口腔医学
题名:
Preliminary X-ray crystallographic analysis of SMU.2055 protein from the caries pathogen Streptococcus mutans
作者: Zhao, Wang-Hong2,3; Zhan, Xiu-Rong3; Gao, Xiong-Zhuo1; Liu, Xiang1; Zhang, Yi-Fei2; Lin, Jiuxiang2; Li, Lan-Fen1; Wei, Shi-Cheng1; Su, Xio-Dong1
刊名: ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS
发表日期: 2010-05-01
DOI: 10.1107/S1744309110010365
卷: 66, 页:530-533
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology ; Biophysics ; Crystallography
研究领域[WOS]: Biochemistry & Molecular Biology ; Biophysics ; Crystallography
关键词[WOS]: CRYSTALS
英文摘要:

The SMU.2055 gene from the major caries pathogen Streptococcus mutans is annotated as a putative acetyltransferase with 163 amino-acid residues. In order to identify its function via structural studies, the SMU.2055 gene was cloned into the expression vector pET28a. Native and SeMet-labelled SMU.2055 proteins with a His(6) tag at the N-terminus were expressed at a high level in Escherichia coli strain BL21 (DE3) and purified to homogeneity by Ni(2+)-chelating affinity chromatography. Diffraction-quality crystals of SeMet-labelled SMU.2055 were obtained using the sitting-drop vapour-diffusion method and diffracted to a resolution of 2.5 angstrom on beamline BL17A at the Photon Factory, Tsukuba, Japan. The crystals belong to the orthorhombic space group C222(1), with unit-cell parameters a = 92.0, b = 95.0, c = 192.2 angstrom. The asymmetric unit contained four molecules, with a solvent content of 57.1%.

语种: 英语
所属项目编号: 30530190 ; 985 ; 211
项目资助者: National Natural Science Foundation of China ; Peking University
WOS记录号: WOS:000277217700012
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61756
Appears in Collections:北京大学口腔医学院_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: 1.Peking Univ, Coll Life Sci, Natl Lab Prot Engn & Plant Genet Engn, Beijing 100871, Peoples R China
2.Peking Univ, Sch Stomatol, Beijing 100081, Peoples R China
3.Lanzhou Univ, Sch Stomatol, Lanzhou 730000, Peoples R China

Recommended Citation:
Zhao, Wang-Hong,Zhan, Xiu-Rong,Gao, Xiong-Zhuo,et al. Preliminary X-ray crystallographic analysis of SMU.2055 protein from the caries pathogen Streptococcus mutans[J]. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS,2010,66:530-533.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhao, Wang-Hong]'s Articles
[Zhan, Xiu-Rong]'s Articles
[Gao, Xiong-Zhuo]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhao, Wang-Hong]‘s Articles
[Zhan, Xiu-Rong]‘s Articles
[Gao, Xiong-Zhuo]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace