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学科主题临床医学
Human spermatozoa contain multiple targets for protein S-nitrosylation: An alternative mechanism of the modulation of sperm function by nitric oxide?
Lefievre, Linda; Chen, Yongjian; Conner, Sarah J.; Scott, Joanna L.; Publicover, Steve J.; Ford, W. Christopher L.; Barratt, Christopher L. R.
关键词human nitric oxide signalling s-nitrosylation spermatozoa
刊名PROTEOMICS
2007-09-01
DOI10.1002/pmic.200700254
7期:17页:3066-3084
收录类别SCI
文章类型Review
WOS标题词Science & Technology
类目[WOS]Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]Biochemistry & Molecular Biology
关键词[WOS]CHANNEL RYANODINE RECEPTOR ; CALCIUM-RELEASE CHANNEL ; ZONA-PELLUCIDA BINDING ; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE ; PROTEOMIC ANALYSIS ; TYROSINE PHOSPHORYLATION ; ACROSOME REACTION ; OXIDATIVE STRESS ; CYCLIC-GMP ; ARABIDOPSIS-THALIANA
英文摘要

Nitric oxide (NO) enhances human sperm motility and capacitation associated with increased protein phosphorylation. NO activates soluble guanylyl cyclase, but can also modify protein function covalently via S-nitrosylation of cysteine. Remarkably, this mechanism remains unexplored in sperm although they depend on post-translational protein modification to achieve changes in function required for fertilisation. Our objective was to identify targets for S-nitrosylation in human sperm. Spermatozoa were incubated with NO donors and S-nitrosylated proteins were identified using the biotin switch assay and a proteomic approach using MS/MS. 240 S-nitrosylated proteins were detected in sperm incubated with S-nitroso-glutathione. Minimal levels were observed in glutathione or untreated samples. Proteins identified consistently based on multiple peptides included established targets for S-nitrosylation in other cells e.g. tubulin, GST and HSPs but also novel targets including A-kinase anchoring protein (AKAP) types 3 and 4, voltage-dependent anion-selective channel protein 3 and semenogelin 1 and 2. In situ localisation revealed S-nitrosylated targets on the postacrosomal region of the head and throughout the flagellum. Potential targets for S-nitrosylation in human sperm include physiologically significant proteins not previously reported in other cells. Their identification will provide novel insight into the mechanism of action of NO in spermatozoa.

语种英语
WOS记录号WOS:000249635200005
Citation statistics
Cited Times:87[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61776
Collection北京大学第三临床医学院_生殖医学中心
作者单位1.Univ Birmingham, Sch Biosci, Birmingham, W Midlands, England
2.Univ Dundee, Ninewells Hosp & Med Sch, Div Maternal & Child Hlth Sci, Dundee DD1 95Y, Scotland
3.Univ Birmingham, Sch Med, Div Reprod & Child Hlth, Reprod Biol & Genet Grp, Birmingham, W Midlands, England
4.Peking Univ, Third Hosp, Ctr Reprod Med, Beijing 100871, Peoples R China
5.Birmingham Womens Hosp, Ctr Human Reprod Sci, Birmingham, W Midlands, England
Recommended Citation
GB/T 7714
Lefievre, Linda,Chen, Yongjian,Conner, Sarah J.,et al. Human spermatozoa contain multiple targets for protein S-nitrosylation: An alternative mechanism of the modulation of sperm function by nitric oxide?[J]. PROTEOMICS,2007,7(17):3066-3084.
APA Lefievre, Linda.,Chen, Yongjian.,Conner, Sarah J..,Scott, Joanna L..,Publicover, Steve J..,...&Barratt, Christopher L. R..(2007).Human spermatozoa contain multiple targets for protein S-nitrosylation: An alternative mechanism of the modulation of sperm function by nitric oxide?.PROTEOMICS,7(17),3066-3084.
MLA Lefievre, Linda,et al."Human spermatozoa contain multiple targets for protein S-nitrosylation: An alternative mechanism of the modulation of sperm function by nitric oxide?".PROTEOMICS 7.17(2007):3066-3084.
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