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学科主题: 临床医学
题名:
Human spermatozoa contain multiple targets for protein S-nitrosylation: An alternative mechanism of the modulation of sperm function by nitric oxide?
作者: Lefievre, Linda; Chen, Yongjian; Conner, Sarah J.; Scott, Joanna L.; Publicover, Steve J.; Ford, W. Christopher L.; Barratt, Christopher L. R.
关键词: human ; nitric oxide ; signalling ; s-nitrosylation ; spermatozoa
刊名: PROTEOMICS
发表日期: 2007-09-01
DOI: 10.1002/pmic.200700254
卷: 7, 期:17, 页:3066-3084
收录类别: SCI
文章类型: Review
WOS标题词: Science & Technology
类目[WOS]: Biochemical Research Methods ; Biochemistry & Molecular Biology
研究领域[WOS]: Biochemistry & Molecular Biology
关键词[WOS]: CHANNEL RYANODINE RECEPTOR ; CALCIUM-RELEASE CHANNEL ; ZONA-PELLUCIDA BINDING ; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE ; PROTEOMIC ANALYSIS ; TYROSINE PHOSPHORYLATION ; ACROSOME REACTION ; OXIDATIVE STRESS ; CYCLIC-GMP ; ARABIDOPSIS-THALIANA
英文摘要:

Nitric oxide (NO) enhances human sperm motility and capacitation associated with increased protein phosphorylation. NO activates soluble guanylyl cyclase, but can also modify protein function covalently via S-nitrosylation of cysteine. Remarkably, this mechanism remains unexplored in sperm although they depend on post-translational protein modification to achieve changes in function required for fertilisation. Our objective was to identify targets for S-nitrosylation in human sperm. Spermatozoa were incubated with NO donors and S-nitrosylated proteins were identified using the biotin switch assay and a proteomic approach using MS/MS. 240 S-nitrosylated proteins were detected in sperm incubated with S-nitroso-glutathione. Minimal levels were observed in glutathione or untreated samples. Proteins identified consistently based on multiple peptides included established targets for S-nitrosylation in other cells e.g. tubulin, GST and HSPs but also novel targets including A-kinase anchoring protein (AKAP) types 3 and 4, voltage-dependent anion-selective channel protein 3 and semenogelin 1 and 2. In situ localisation revealed S-nitrosylated targets on the postacrosomal region of the head and throughout the flagellum. Potential targets for S-nitrosylation in human sperm include physiologically significant proteins not previously reported in other cells. Their identification will provide novel insight into the mechanism of action of NO in spermatozoa.

语种: 英语
WOS记录号: WOS:000249635200005
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61776
Appears in Collections:北京大学第三临床医学院_生殖医学中心_期刊论文

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作者单位: 1.Univ Birmingham, Sch Biosci, Birmingham, W Midlands, England
2.Univ Dundee, Ninewells Hosp & Med Sch, Div Maternal & Child Hlth Sci, Dundee DD1 95Y, Scotland
3.Univ Birmingham, Sch Med, Div Reprod & Child Hlth, Reprod Biol & Genet Grp, Birmingham, W Midlands, England
4.Peking Univ, Third Hosp, Ctr Reprod Med, Beijing 100871, Peoples R China
5.Birmingham Womens Hosp, Ctr Human Reprod Sci, Birmingham, W Midlands, England

Recommended Citation:
Lefievre, Linda,Chen, Yongjian,Conner, Sarah J.,et al. Human spermatozoa contain multiple targets for protein S-nitrosylation: An alternative mechanism of the modulation of sperm function by nitric oxide?[J]. PROTEOMICS,2007,7(17):3066-3084.
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