北京大学医学部机构知识库
Advanced  
IR@PKUHSC  > 北京大学第二临床医学院  > 眼科  > 期刊论文
学科主题: 临床医学
题名:
Effects of apelin and vascular endothelial growth factor on central retinal vein occlusion in monkey eyes intravitreally injected with bevacizumab: a preliminary study
作者: Zhao, Tong; Lu, Qiang; Tao, Yong; Liang, Xiao-Ying; Wang, Kai; Jiang, Yan-Rong
刊名: MOLECULAR VISION
发表日期: 2011-04-27
卷: 17, 期:117, 页:1044-1054
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Biochemistry & Molecular Biology ; Ophthalmology
研究领域[WOS]: Biochemistry & Molecular Biology ; Ophthalmology
关键词[WOS]: PROLIFERATIVE DIABETIC-RETINOPATHY ; MACULAR EDEMA ; IMMUNOHISTOCHEMICAL LOCALIZATION ; IRIS NEOVASCULARIZATION ; ANGIOGENIC FACTOR ; PRIMATE EYE ; RECEPTOR ; LIGAND ; TRIAMCINOLONE ; EXPRESSION
英文摘要:

Purpose: To examine the intraocular distribution of bevacizumab at four weeks after intravitreal bevacizumab (IVB) injection and to investigate the effects of IVB on apelin and vascular endothelial growth factor (VEGF) in the central retinal vein occlusion (CRVO) of monkey eyes.

Methods: Direct laser coagulation was performed on all branch retinal veins in the right eyes of six Rhesus monkeys to establish a CRVO model. The eyes of the first three monkeys were enucleated one week, two weeks, and 24 weeks after the establishment of the CRVO model; this was the CRVO group. Subsequently, IVB was injected into the eyes of the last three monkeys one week, two weeks, and 24 weeks after laser coagulation; this was the IVB group. The left eye of the first monkey was used as normal control. Immunohistochemistry and reverse-transcription PCR was used to examine the expression of apelin and VEGF. The penetration of bevacizumab into the retina and iris was investigated by fluorescence immunostaining.

Results: Immunoreactivity for bevacizumab could be detected in the vessel walls of the iris and choroid on day 28 after injecting IVB: apelin and VEGF staining had been more prominent than normal in the CRVO eye, but these decreased following IVB injection. Expression of apelin mRNA (p<0.01) was lower in the IVB group than the CRVO group and did not vary significantly between groups.

Conclusions: Bevacizumab could be detected in the iris and choroid after four weeks of intravitreal injection. Apelin may be partially suppressed by bevacizumab, and it may play a role in retinal neovascularization during the development of CRVO.

语种: 英语
所属项目编号: 30901639 ; 81041005 ; 2009B04 ; 7112142 ; Z08000303220801 ; 20100001110073
项目资助者: National Natural Science Foundation of China ; Beijing Novel Program ; Beijing Natural Science Foundation ; Science and Technology Project of Beijing ; Research Fund for the Doctoral Program of Higher Education of China ; EFSD/CDS/Lilly
WOS记录号: WOS:000289983700001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61788
Appears in Collections:北京大学第二临床医学院_眼科_期刊论文

Files in This Item:

There are no files associated with this item.


作者单位: Peking Univ, Dept Ophthalmol, Peoples Hosp, Key Lab Vis Loss & Restorat,Minist Educ, Beijing 100044, Peoples R China

Recommended Citation:
Zhao, Tong,Lu, Qiang,Tao, Yong,et al. Effects of apelin and vascular endothelial growth factor on central retinal vein occlusion in monkey eyes intravitreally injected with bevacizumab: a preliminary study[J]. MOLECULAR VISION,2011,17(117):1044-1054.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[Zhao, Tong]'s Articles
[Lu, Qiang]'s Articles
[Tao, Yong]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[Zhao, Tong]‘s Articles
[Lu, Qiang]‘s Articles
[Tao, Yong]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Copyright © 2007-2017  北京大学医学部 - Feedback
Powered by CSpace