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Effects of apelin and vascular endothelial growth factor on central retinal vein occlusion in monkey eyes intravitreally injected with bevacizumab: a preliminary study
Zhao, Tong; Lu, Qiang; Tao, Yong; Liang, Xiao-Ying; Wang, Kai; Jiang, Yan-Rong
刊名MOLECULAR VISION
2011-04-27
17期:117页:1044-1054
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Ophthalmology
资助者National Natural Science Foundation of China ; Beijing Novel Program ; Beijing Natural Science Foundation ; Science and Technology Project of Beijing ; Research Fund for the Doctoral Program of Higher Education of China ; EFSD/CDS/Lilly ; National Natural Science Foundation of China ; Beijing Novel Program ; Beijing Natural Science Foundation ; Science and Technology Project of Beijing ; Research Fund for the Doctoral Program of Higher Education of China ; EFSD/CDS/Lilly
研究领域[WOS]Biochemistry & Molecular Biology ; Ophthalmology
关键词[WOS]PROLIFERATIVE DIABETIC-RETINOPATHY ; MACULAR EDEMA ; IMMUNOHISTOCHEMICAL LOCALIZATION ; IRIS NEOVASCULARIZATION ; ANGIOGENIC FACTOR ; PRIMATE EYE ; RECEPTOR ; LIGAND ; TRIAMCINOLONE ; EXPRESSION
英文摘要

Purpose: To examine the intraocular distribution of bevacizumab at four weeks after intravitreal bevacizumab (IVB) injection and to investigate the effects of IVB on apelin and vascular endothelial growth factor (VEGF) in the central retinal vein occlusion (CRVO) of monkey eyes.

Methods: Direct laser coagulation was performed on all branch retinal veins in the right eyes of six Rhesus monkeys to establish a CRVO model. The eyes of the first three monkeys were enucleated one week, two weeks, and 24 weeks after the establishment of the CRVO model; this was the CRVO group. Subsequently, IVB was injected into the eyes of the last three monkeys one week, two weeks, and 24 weeks after laser coagulation; this was the IVB group. The left eye of the first monkey was used as normal control. Immunohistochemistry and reverse-transcription PCR was used to examine the expression of apelin and VEGF. The penetration of bevacizumab into the retina and iris was investigated by fluorescence immunostaining.

Results: Immunoreactivity for bevacizumab could be detected in the vessel walls of the iris and choroid on day 28 after injecting IVB: apelin and VEGF staining had been more prominent than normal in the CRVO eye, but these decreased following IVB injection. Expression of apelin mRNA (p<0.01) was lower in the IVB group than the CRVO group and did not vary significantly between groups.

Conclusions: Bevacizumab could be detected in the iris and choroid after four weeks of intravitreal injection. Apelin may be partially suppressed by bevacizumab, and it may play a role in retinal neovascularization during the development of CRVO.

语种英语
所属项目编号30901639 ; 81041005 ; 2009B04 ; 7112142 ; Z08000303220801 ; 20100001110073
资助者National Natural Science Foundation of China ; Beijing Novel Program ; Beijing Natural Science Foundation ; Science and Technology Project of Beijing ; Research Fund for the Doctoral Program of Higher Education of China ; EFSD/CDS/Lilly ; National Natural Science Foundation of China ; Beijing Novel Program ; Beijing Natural Science Foundation ; Science and Technology Project of Beijing ; Research Fund for the Doctoral Program of Higher Education of China ; EFSD/CDS/Lilly
WOS记录号WOS:000289983700001
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61788
专题北京大学第二临床医学院_眼科
作者单位Peking Univ, Dept Ophthalmol, Peoples Hosp, Key Lab Vis Loss & Restorat,Minist Educ, Beijing 100044, Peoples R China
推荐引用方式
GB/T 7714
Zhao, Tong,Lu, Qiang,Tao, Yong,et al. Effects of apelin and vascular endothelial growth factor on central retinal vein occlusion in monkey eyes intravitreally injected with bevacizumab: a preliminary study[J]. MOLECULAR VISION,2011,17(117):1044-1054.
APA Zhao, Tong,Lu, Qiang,Tao, Yong,Liang, Xiao-Ying,Wang, Kai,&Jiang, Yan-Rong.(2011).Effects of apelin and vascular endothelial growth factor on central retinal vein occlusion in monkey eyes intravitreally injected with bevacizumab: a preliminary study.MOLECULAR VISION,17(117),1044-1054.
MLA Zhao, Tong,et al."Effects of apelin and vascular endothelial growth factor on central retinal vein occlusion in monkey eyes intravitreally injected with bevacizumab: a preliminary study".MOLECULAR VISION 17.117(2011):1044-1054.
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