IR@PKUHSC  > 北京大学第二临床医学院  > 儿科
学科主题临床医学
Localization of cystathionine beta synthase in mice ovaries and its expression profile during follicular development
Liang Rong; Yu Wei-dong; Du Jun-bao; Yang Li-jun; Shang Mei; Guo Jing-zhu
关键词cystathionine beta synthase oocytes ovarian follicles homocysteine
刊名CHINESE MEDICAL JOURNAL
2006-11-20
119期:22页:1877-1883
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Medicine, General & Internal
研究领域[WOS]General & Internal Medicine
关键词[WOS]MESSENGER-RIBONUCLEIC-ACID ; CUMULUS-OOCYTE COMPLEXES ; KIT-LIGAND EXPRESSION ; GRANULOSA-CELLS ; IN-VITRO ; MOUSE ; GLUTATHIONE ; FLUID ; HOMOCYSTEINE ; FOLLICLES
英文摘要

Background In vitro fertilization (IVF) researches have suggested that cystathionine P synthase (CBS) is involved in oocyte development. However, little is known about the regional and cellular expression patterns of CBS in the ovary. The purpose of this study was to analyze the localization of CBS in mice ovaries and to investigate the expression profile during follicular development.

Methods We used in situ hybridization and immunohistochemical analysis to determine CBS expression in the ovaries of female Balb/c mice. Then the follicles were collected from F1 (C57BL X Balb/c) mice and cultured in vitro. With the method of semi-quantitative RT-PCR, we also investigated the expression profile of CBS during follicular development.

Results CBS was absent in the oocytes, although it was ubiquitously expressed in the ovary with the strongest expression in follicular cells at all stages. In late antral follicles, CBS expression was markedly higher in granulosa cells located close to the antrum and in cumulus cells around the oocyte. The semi-quantitative RT-PCR showed that CBS mRNA was detected in follicles at all stages in vitro. In cumulus-oocyte complexes superovulated, CBS expression also increased rapidly.

Conclusions CBS was located mainly in the follicular cells in the ovaries. The level of CBS expression is high in follicles during folliculogenesis in mice. Differences in the CBS expression profile between oocyte and follicular cells suggest a role for CBS as a mediator in interactions between oocyte and granulosa cells.

语种英语
WOS记录号WOS:000242328700006
Citation statistics
Cited Times:12[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61790
Collection北京大学第二临床医学院_儿科
作者单位1.Peking Univ, Dept Pediat, Peoples Hosp, Ctr Lab, Beijing 100044, Peoples R China
2.Peking Univ, First Hosp, Dept Pediat, Beijing 100034, Peoples R China
Recommended Citation
GB/T 7714
Liang Rong,Yu Wei-dong,Du Jun-bao,et al. Localization of cystathionine beta synthase in mice ovaries and its expression profile during follicular development[J]. CHINESE MEDICAL JOURNAL,2006,119(22):1877-1883.
APA Liang Rong,Yu Wei-dong,Du Jun-bao,Yang Li-jun,Shang Mei,&Guo Jing-zhu.(2006).Localization of cystathionine beta synthase in mice ovaries and its expression profile during follicular development.CHINESE MEDICAL JOURNAL,119(22),1877-1883.
MLA Liang Rong,et al."Localization of cystathionine beta synthase in mice ovaries and its expression profile during follicular development".CHINESE MEDICAL JOURNAL 119.22(2006):1877-1883.
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