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Identification of the involvement of LOXL4 in generation of keratocystic odontogenic tumors by RNA-Seq analysis
Jiang, Wei-Peng1; Sima, Zi-Han1; Wang, Hai-Cheng1; Zhang, Jian-Yun1; Sun, Li-Sha2; Chen, Feng2; Li, Tie-Jun1
关键词angiogenesis keratocystic odontogenic tumor lysyl oxidase-like 4 RNA-sequencing tumor stromal fibroblast
刊名INTERNATIONAL JOURNAL OF ORAL SCIENCE
2014-03-01
DOI10.1038/ijos.2013.96
6期:1页:31-38
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Dentistry, Oral Surgery & Medicine
资助者National Natural Science Foundation of China ; Doctoral Fund of Ministry of Education of China ; National Natural Science Foundation of China ; Doctoral Fund of Ministry of Education of China
研究领域[WOS]Dentistry, Oral Surgery & Medicine
关键词[WOS]COLLAGEN MESSENGER-RNAS ; CELL CARCINOMA ; DENTIGEROUS CYSTS ; NECK-CANCER ; ANGIOGENESIS ; HEAD ; VESSELS ; ANTIGEN ; FIBERS ; WALLS
英文摘要

Keratocystic odontogenic tumors (KCOT) are benign, locally aggressive intraosseous tumors of odontogenic origin. KCOT have a higher stromal microvessel density (MVD) than dentigerous cysts (DC) and normal oral mucosa. To identify genes in the stroma of KCOT involved in tumor development and progression, RNA sequencing (RNA-Seq) was performed using samples from KCOT and primary stromal fibroblasts isolated from gingival tissues. Seven candidate genes that possess a function potentially related to KCOT progression were selected and their expression levels were confirmed by quantitative PCR, immunohistochemistry and enzyme-linked immunosorbent assay. Expression of lysyl oxidase-like 4 (LOXL4), the only candidate gene that encodes a secreted protein, was enhanced at both the mRNA and protein levels in KCOT stromal tissues and primary KCOT stromal fibroblasts compared to control tissues and primary fibroblasts (P<0.05). In vitro, high expression of LOXL4 could enhance proliferation and migration of the human umbilical vein endothelial cells (HUVECs). There was a significant, positive correlation between LOXL4 protein expression and MVD in stroma of KCOT and control tissues (r=0.882). These data suggest that abnormal expression of LOXL4 of KCOT may enhance angiogenesis in KCOT, which may help to promote the locally aggressive biological behavior of KCOT.

语种英语
所属项目编号81030018 ; 30872900 ; 30901680 ; 20120001110043
资助者National Natural Science Foundation of China ; Doctoral Fund of Ministry of Education of China ; National Natural Science Foundation of China ; Doctoral Fund of Ministry of Education of China
WOS记录号WOS:000332429800006
引用统计
被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61799
专题北京大学口腔医学院_口腔病理科
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Oral Pathol, Beijing 100081, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Cent Lab, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Jiang, Wei-Peng,Sima, Zi-Han,Wang, Hai-Cheng,et al. Identification of the involvement of LOXL4 in generation of keratocystic odontogenic tumors by RNA-Seq analysis[J]. INTERNATIONAL JOURNAL OF ORAL SCIENCE,2014,6(1):31-38.
APA Jiang, Wei-Peng.,Sima, Zi-Han.,Wang, Hai-Cheng.,Zhang, Jian-Yun.,Sun, Li-Sha.,...&Li, Tie-Jun.(2014).Identification of the involvement of LOXL4 in generation of keratocystic odontogenic tumors by RNA-Seq analysis.INTERNATIONAL JOURNAL OF ORAL SCIENCE,6(1),31-38.
MLA Jiang, Wei-Peng,et al."Identification of the involvement of LOXL4 in generation of keratocystic odontogenic tumors by RNA-Seq analysis".INTERNATIONAL JOURNAL OF ORAL SCIENCE 6.1(2014):31-38.
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