IR@PKUHSC  > 北京大学基础医学院  > 免疫学系
学科主题基础医学
Immune receptors for polysaccharides from Ganoderma lucidum
Shao, BM; Dai, H; Xu, W; Lin, ZB; Gao, XM
关键词Ganoderma lucidum polysaccharide receptor TLR4 immunomodulation
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2004-10-08
DOI10.1016/j.bbrc.2004.08.069
323期:1页:133-141
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]NF-KAPPA-B ; PLATYCODON-GRANDIFLORUM ; INNATE IMMUNITY ; CELL-CULTURE ; MACROPHAGES ; ACTIVATION ; ANTITUMOR ; STIMULATION ; LYMPHOCYTES ; MUSHROOMS
英文摘要

This study was designed to identify and characterize the immune receptors for polysaccharides from Ganoderma lucidum, a Chinese medicinal fungus that exhibits anti-tumor activities via enhancing host immunity. We herein demonstrate that G. lucidum polysaccharides (GLPS) activated BALB/c mouse B cells and macrophages, but not T cells, in vitro. However, GLPS was unable to activate splenic B cells from C3H/HeJ mice that have a mutated TLR4 molecule (incapable of signal transduction) in proliferation assays. Rat anti-mouse TLR4 monoclonal antibody (Ab) inhibited the proliferation of BALB/c mouse B cells under GLPS stimulation. Combination of Abs against mouse TLR4 and immunoglobulin (Ig) achieved almost complete inhibition of GLPS-induced B cell proliferation, implying that both membrane Ig and TLR4 are required for GLPS-mediated B cell activation. In addition, GLPS significantly inhibited the binding of mouse peritoneal macrophages with polysaccharides from Astragalus membranaceus, which is known to bind directly with TLR4 on macrophage surface. Moreover, GLPS induced IL-1beta production by peritoneal macrophages from BALB/c, but not C3H/HeJ, mice, suggesting that TLR4 is also involved in GLPS-mediated macrophage activation. We Further identified a unique 31 kDa serum protein and two intracellular proteins (ribosomal protein S7 and a transcriptional coactivator) capable of binding with GLPS in co-precipitation experiments. Our results may have important implications for our understanding on the molecular mechanisms of immunopotentiating polysaccharides from traditional Chinese medicine. (C) 2004 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000223965300021
Citation statistics
Cited Times:78[WOS]   [WOS Record]     [Related Records in WOS]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61805
Collection北京大学基础医学院_免疫学系
作者单位1.Peking Univ, Sch Basic Med Sci, Hlth Sci Ctr, Dept Immunol, Beijing 100871, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Hlth Sci Ctr, Dept Pharmacol, Beijing 100871, Peoples R China
Recommended Citation
GB/T 7714
Shao, BM,Dai, H,Xu, W,et al. Immune receptors for polysaccharides from Ganoderma lucidum[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2004,323(1):133-141.
APA Shao, BM,Dai, H,Xu, W,Lin, ZB,&Gao, XM.(2004).Immune receptors for polysaccharides from Ganoderma lucidum.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,323(1),133-141.
MLA Shao, BM,et al."Immune receptors for polysaccharides from Ganoderma lucidum".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 323.1(2004):133-141.
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