IR@PKUHSC  > 基础医学院  > 免疫学系
学科主题基础医学
Immune receptors for polysaccharides from Ganoderma lucidum
Shao, BM; Dai, H; Xu, W; Lin, ZB; Gao, XM
关键词Ganoderma lucidum polysaccharide receptor TLR4 immunomodulation
刊名BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
2004-10-08
DOI10.1016/j.bbrc.2004.08.069
323期:1页:133-141
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Biochemistry & Molecular Biology ; Biophysics
研究领域[WOS]Biochemistry & Molecular Biology ; Biophysics
关键词[WOS]NF-KAPPA-B ; PLATYCODON-GRANDIFLORUM ; INNATE IMMUNITY ; CELL-CULTURE ; MACROPHAGES ; ACTIVATION ; ANTITUMOR ; STIMULATION ; LYMPHOCYTES ; MUSHROOMS
英文摘要

This study was designed to identify and characterize the immune receptors for polysaccharides from Ganoderma lucidum, a Chinese medicinal fungus that exhibits anti-tumor activities via enhancing host immunity. We herein demonstrate that G. lucidum polysaccharides (GLPS) activated BALB/c mouse B cells and macrophages, but not T cells, in vitro. However, GLPS was unable to activate splenic B cells from C3H/HeJ mice that have a mutated TLR4 molecule (incapable of signal transduction) in proliferation assays. Rat anti-mouse TLR4 monoclonal antibody (Ab) inhibited the proliferation of BALB/c mouse B cells under GLPS stimulation. Combination of Abs against mouse TLR4 and immunoglobulin (Ig) achieved almost complete inhibition of GLPS-induced B cell proliferation, implying that both membrane Ig and TLR4 are required for GLPS-mediated B cell activation. In addition, GLPS significantly inhibited the binding of mouse peritoneal macrophages with polysaccharides from Astragalus membranaceus, which is known to bind directly with TLR4 on macrophage surface. Moreover, GLPS induced IL-1beta production by peritoneal macrophages from BALB/c, but not C3H/HeJ, mice, suggesting that TLR4 is also involved in GLPS-mediated macrophage activation. We Further identified a unique 31 kDa serum protein and two intracellular proteins (ribosomal protein S7 and a transcriptional coactivator) capable of binding with GLPS in co-precipitation experiments. Our results may have important implications for our understanding on the molecular mechanisms of immunopotentiating polysaccharides from traditional Chinese medicine. (C) 2004 Elsevier Inc. All rights reserved.

语种英语
WOS记录号WOS:000223965300021
引用统计
被引频次:77[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
版本出版稿
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61805
专题基础医学院_免疫学系
作者单位1.Peking Univ, Sch Basic Med Sci, Hlth Sci Ctr, Dept Immunol, Beijing 100871, Peoples R China
2.Peking Univ, Sch Basic Med Sci, Hlth Sci Ctr, Dept Pharmacol, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Shao, BM,Dai, H,Xu, W,et al. Immune receptors for polysaccharides from Ganoderma lucidum[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,2004,323(1):133-141.
APA Shao, BM,Dai, H,Xu, W,Lin, ZB,&Gao, XM.(2004).Immune receptors for polysaccharides from Ganoderma lucidum.BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,323(1),133-141.
MLA Shao, BM,et al."Immune receptors for polysaccharides from Ganoderma lucidum".BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 323.1(2004):133-141.
条目包含的文件 下载所有文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
1-s2.0-S0006291X0401(1255KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 下载
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Shao, BM]的文章
[Dai, H]的文章
[Xu, W]的文章
百度学术
百度学术中相似的文章
[Shao, BM]的文章
[Dai, H]的文章
[Xu, W]的文章
必应学术
必应学术中相似的文章
[Shao, BM]的文章
[Dai, H]的文章
[Xu, W]的文章
相关权益政策
暂无数据
收藏/分享
文件名: 1-s2.0-S0006291X04018005-main.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。