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学科主题口腔医学
The Pro-Apoptotic and Pro-Inflammatory Effects of Calprotectin on Human Periodontal Ligament Cells
Zheng, Yunfei1,2; Hou, Jianxia2; Peng, Lei3; Zhang, Xin4; Jia, Lingfei1,5; Wang, Xian′ e(2); Wei, Shicheng1; Meng, Huanxin2
刊名PLOS ONE
2014-10-22
DOI10.1371/journal.pone.0110421
9期:10
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Multidisciplinary Sciences
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
研究领域[WOS]Science & Technology - Other Topics
关键词[WOS]GINGIVAL CREVICULAR FLUID ; GENERALIZED AGGRESSIVE PERIODONTITIS ; DEPENDENT PATHWAY ; S100A8 ; PROTEIN ; NEUTROPHILS ; MRP14 ; DISEASE ; CANCER ; S100A8/S100A9
英文摘要

Calprotectin, a heterodimer of S100A8 and S100A9 subunits, is associated with inflammatory disorders such as rheumatoid arthritis and cystic fibrosis. Although calprotectin levels are increased significantly in the gingival crevicular fluid (GCF) of periodontitis patients, its effects on periodontal ligament cells (PDLCs) remain largely unknown. The aim of this study was to evaluate calprotectin levels in the GCF of generalized aggressive periodontitis (AgP) patients and to investigate the effects of recombinant human calprotectin (rhS100A8/A9) and its subunits (rhS100A8 and rhS100A9) in PDLCs. Both the concentration and amount of crevicular calprotectin were significantly higher in the AgP group compared with healthy controls. In addition, the GCF calprotectin levels were correlated positively with clinical periodontal parameters including bleeding index, probing depth, and clinical attachment loss. rhS100A8/A9 promoted cell apoptosis, whereas rhS100A8 and rhS100A9 individually exerted little effect on apoptosis in PDLCs. rhS100A9 and rhS100A8/A9 increased the activation of nuclear factor-kappa B (NF-kappa B) by promoting the nuclear translocation of p65 in PDLCs, subsequently inducing expression of the pro-inflammatory cytokines IL-6, IL-8, TNF alpha, and COX2. Treatment with an NF-kappa B inhibitor partially reversed the rhS100A9-and rhS100A8/A9-induced upregulation of the pro-inflammatory cytokines. rhS100A9, and not rhS100A8, was mainly responsible for the pro-inflammatory role of calprotectin. Collectively, our results suggest that calprotectin promotes apoptosis and the inflammatory response in PDLCs via rhS100A9. These findings might help identify novel treatments for periodontitis.

语种英语
所属项目编号30271411 ; 30471882 ; 30973319
资助者National Natural Science Foundation of China ; National Natural Science Foundation of China
WOS记录号WOS:000343674800041
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61809
专题北京大学口腔医学院_牙周科
作者单位1.Peking Univ, Sch & Hosp Stomatol, Dept Oral & Maxillofacial Surg, Beijing 100871, Peoples R China
2.Peking Univ, Sch & Hosp Stomatol, Dept Periodontol, Beijing 100871, Peoples R China
3.Capital Med Univ, Beijing Friendship Hosp, Dept Stomatol, Beijing, Peoples R China
4.Chinese Acad Med Sci, Peking Union Med Coll Hosp, Dept Stomatol, Beijing 100730, Peoples R China
5.Peking Univ, Sch & Hosp Stomatol, Mol Biol Lab, Beijing 100871, Peoples R China
推荐引用方式
GB/T 7714
Zheng, Yunfei,Hou, Jianxia,Peng, Lei,et al. The Pro-Apoptotic and Pro-Inflammatory Effects of Calprotectin on Human Periodontal Ligament Cells[J]. PLOS ONE,2014,9(10).
APA Zheng, Yunfei.,Hou, Jianxia.,Peng, Lei.,Zhang, Xin.,Jia, Lingfei.,...&Meng, Huanxin.(2014).The Pro-Apoptotic and Pro-Inflammatory Effects of Calprotectin on Human Periodontal Ligament Cells.PLOS ONE,9(10).
MLA Zheng, Yunfei,et al."The Pro-Apoptotic and Pro-Inflammatory Effects of Calprotectin on Human Periodontal Ligament Cells".PLOS ONE 9.10(2014).
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