IR@PKUHSC  > 基础医学院  > 细胞生物学系
学科主题基础医学
Inducible expression of stem cell associated intermediate filament nestin reveals an important role in glioblastoma carcinogenesis
Lu, Wen Jing1,2; Lan, Feng1,3; He, Qihua1; Lee, Andrew3; Tang, Chad Z.3; Dong, Lin1; Lan, Baojin4; Ma, Xiaowen1; Wu, Joseph C.3; Shen, Li1
关键词nestin glioblastoma shRNA carcinogenesis
刊名INTERNATIONAL JOURNAL OF CANCER
2011-01-15
DOI10.1002/ijc.25586
128期:2页:343-351
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Oncology
资助者Fujian Ministry of Education Project ; Fujian Ministry of Education Project
研究领域[WOS]Oncology
关键词[WOS]CENTRAL-NERVOUS-SYSTEM ; DIFFERENT TUMORS ; PROTEIN ; CANCER ; MARKER ; GLIOMA ; IDENTIFICATION ; ORGANIZATION ; VIMENTIN
英文摘要

The intermediate filament nestin is transiently expressed in neural stem/progenitor cells during the development of central nervous system. Recently, increasing evidence has shown that upregulation of nestin is related to malignancy of several cancers, especially glioblastoma. However, the function of nestin in carcinogenesis remains unclear. In this study, we investigated the role of nestin in glioblastoma carcinogenesis by comparing subclones of rat C6 glioblastoma cells that were either high or low for nestin expression. We found that while nestin expression did not influence the in vitro proliferation of glioblastoma cells, subclones characterized by high levels of nestin formed tumors in vivo at significantly faster rates than subclones with low expression. Importantly, C6 subclones that expressed nestin at low levels in vitro were also found to give rise to tumors highly positive for the protein, suggesting that induction of nestin plays an important role in glioblastoma carcinogenesis. Derivation of nestin positive tumors from nestin negative human U87 glioblastoma cells in immunodeficient mice further confirmed that a switch to positive expression of nestin is fundamental to the course of glioblastoma development. Blocking the expression of nestin in glioblastoma tumors via intratumor injection of shRNA significantly slowed tumor growth and volume. These results demonstrated that nestin plays a crucial role in development of glioblastoma and may potentially be targeted for treatment of the disease.

语种英语
所属项目编号JA08257
资助者Fujian Ministry of Education Project ; Fujian Ministry of Education Project
WOS记录号WOS:000285263100010
引用统计
被引频次:12[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61810
专题基础医学院_细胞生物学系
作者单位1.Peking Univ, Hlth Sci Ctr, Dept Cell Biol, Beijing 100191, Peoples R China
2.Univ Hong Kong, Dept Surg, Li Ka Shing Fac Med, Hong Kong, Hong Kong, Peoples R China
3.Stanford Univ, Dept Med, Sch Med, Stanford, CA 94305 USA
4.Fujian Coll Med Occupat & Technol, Dept Common Basic, Fuzhou, Peoples R China
推荐引用方式
GB/T 7714
Lu, Wen Jing,Lan, Feng,He, Qihua,et al. Inducible expression of stem cell associated intermediate filament nestin reveals an important role in glioblastoma carcinogenesis[J]. INTERNATIONAL JOURNAL OF CANCER,2011,128(2):343-351.
APA Lu, Wen Jing.,Lan, Feng.,He, Qihua.,Lee, Andrew.,Tang, Chad Z..,...&Shen, Li.(2011).Inducible expression of stem cell associated intermediate filament nestin reveals an important role in glioblastoma carcinogenesis.INTERNATIONAL JOURNAL OF CANCER,128(2),343-351.
MLA Lu, Wen Jing,et al."Inducible expression of stem cell associated intermediate filament nestin reveals an important role in glioblastoma carcinogenesis".INTERNATIONAL JOURNAL OF CANCER 128.2(2011):343-351.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Lu, Wen Jing]的文章
[Lan, Feng]的文章
[He, Qihua]的文章
百度学术
百度学术中相似的文章
[Lu, Wen Jing]的文章
[Lan, Feng]的文章
[He, Qihua]的文章
必应学术
必应学术中相似的文章
[Lu, Wen Jing]的文章
[Lan, Feng]的文章
[He, Qihua]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。