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学科主题: 临床医学
题名:
Overexpression of caspase-1 in pancreatic disorders: Implications for a function besides apoptosis
作者: Ramadani, M; Gansauge, F; Schlosser, S; Yang, Y; Beger, HG; Gansauge, S
关键词: caspase-1 ; interleukin 1 beta-converting enzyme ; apoptosis ; pancreatic cancer ; chronic pancreatitis
刊名: JOURNAL OF GASTROINTESTINAL SURGERY
发表日期: 2001-07-01
卷: 5, 期:4, 页:352-358
收录类别: ISTP ; SCI
文章类型: Proceedings Paper
WOS标题词: Science & Technology
类目[WOS]: Gastroenterology & Hepatology ; Surgery
研究领域[WOS]: Gastroenterology & Hepatology ; Surgery
关键词[WOS]: EPIDERMAL GROWTH-FACTOR ; CYCLIN D1 EXPRESSION ; DEATH GENE CED-3 ; ICE-LIKE PROTEASE ; CELL-DEATH ; INTERLEUKIN-1-BETA-CONVERTING ENZYME ; IL-1-BETA-CONVERTING ENZYME ; MAMMALIAN HOMOLOG ; FACTOR RECEPTOR ; POOR-PROGNOSIS
英文摘要:

The caspases are known to play a crucial role in the triggering and execution of apoptosis in a variety of cell types. We assessed the expression of caspase-1 in 42 pancreatic cancer tissue samples, 38 chronic pancreatitis specimens, and nine normal pancreatic tissues by immunohistochemistry and Western blot analysis. We found a clear overexpression of caspase-1 in both disorders, but differences in the expression patterns in distinct morphologic compartments. Pancreatic cancer tissue showed a clear cytoplasmatic overexpression of caspase-1 in tumor cells in 71 % of the tumors, whereas normal pancreatic tissue showed only occasional immunoreactivity. In chronic pancreatitis an overexpression of caspase-1 was found in atrophic acinar cells (89%), hyperplastic ducts (87%), and dedifferentiating acinar cells (84%). Although in atrophic cells a clear nuclear expression was found, hyperplastic ducts and dedifferentiating acinar cells showed clear cytoplasmic expression. Western blot analysis revealed a marked expression of the 45 kDa precursor of caspase-1 in pancreatic cancer and chronic pancreatitis (80% and 86%, respectively). Clear bands at 30 kDa, suggested to represent the p10-p20 heterodimer of active caspase-1, were found in 60% of the cancer tissue and 14% of the pancreatitis tissue specimens. Since we found a highly significant correlation between cytoplasm overexpression of caspase-1 in pancreatic cancer and overexpression of the known prognostic factors cyclin D1, epidermal growth factor, and epidermal growth factor receptor, it is plausible that caspase-1 has a yet unknown function in proliferative processes in addition to its well-known role in the apoptotic pathway.

语种: 英语
WOS记录号: WOS:000170717300004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61817
Appears in Collections:北京大学第三临床医学院_普通外科_期刊论文

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作者单位: 1.Univ Ulm, Dept Gen Surg, Div Mol Oncol, D-89075 Ulm, Germany
2.Beijing Med Univ, Dept Surg, Beijing 100083, Peoples R China

Recommended Citation:
Ramadani, M,Gansauge, F,Schlosser, S,et al. Overexpression of caspase-1 in pancreatic disorders: Implications for a function besides apoptosis[J]. JOURNAL OF GASTROINTESTINAL SURGERY,2001,5(4):352-358.
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