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学科主题: 基础医学
题名:
PDGF mediates derivation of human embryonic germ cells
作者: Li, Yang1,2,3; Hong, Wan Xing4,5; Lan, Baojin6; Xu, Xiaoyan3; Liu, Yinan1,2; Kong, Lin3; Li, Yaxuan3; Zhou, Shixin1,2; Liu, Ying7; Feng, Ruopeng8; Jiang, Sibo1,2; He, Qihua9; Tan, Jichun3
关键词: PDGF ; Human embryonic germ cells ; Derivation
刊名: DIFFERENTIATION
发表日期: 2013-11-01
DOI: 10.1016/j.diff.2013.11.002
卷: 86, 期:4-5, 页:141-148
收录类别: SCI
文章类型: Article
WOS标题词: Science & Technology
类目[WOS]: Cell Biology ; Developmental Biology
研究领域[WOS]: Cell Biology ; Developmental Biology
关键词[WOS]: PLURIPOTENT STEM-CELLS ; GROWTH-FACTOR ; IN-VITRO ; CULTURE ; SPHINGOSINE-1-PHOSPHATE ; BLASTOCYSTS ; EXPRESSION ; PROMOTES ; MARKERS ; ROLES
英文摘要:

Human embryonic germ cells (hEGCs) are a valuable and underutilized source of pluripotent stem cells. Unlike embryonic stem cells, which have been extensively studied, little is known about the factors that regulate hEGC derivation and maintenance. This study demonstrates for the first time a central role for selective activation of PDGFR signaling in the derivation and maintenance of pluripotency in hEGCs. In the study, hEGCs were found to express PDGF receptor a at high levels compared to human embryonic stem cells (hESCs). PDGF significantly improved formation of alkaline phosphatase (AP) positive hEGC colonies. We subsequently determined that PDGF activates the phosphatidylinositol-3-kinase (P13K) pathway as phosphorylation of AKT was up-regulated in response to PDGF. Furthermore, inhibition of PI3K signaling using small molecular inhibitor LY294002 led to significantly decreased AP positive hEGC colony formation whereas inhibition of MAPK pathway using U0126 had a negligible effect. We established a primary mechanism for PDGF mediated derivation and maintenance of hEGCs by demonstrating that OCT4 was upregulated and PTEN was suppressed in a dose dependent manner in response to PDGF. (C) 2014 International Society of Differentiation. Published by Elsevier BM. All rights reserved.

语种: 英语
所属项目编号: 31000653 ; 5112019 ; 20100001120044 ; 2012MS1142
项目资助者: National Natural Science Foundation of China (NSFC) Projects ; Beijing Natural Science Foundation Project ; Ph.D. Programs Foundation of Ministry of Education of China ; Inner Mongolia Natural Science Foundation
WOS记录号: WOS:000335297100001
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.bjmu.edu.cn/handle/400002259/61818
Appears in Collections:基础医学院_细胞生物学系_期刊论文

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作者单位: 1.Stanford Sch Med, Dept Radiol, Stanford, CA 94305 USA
2.Stanford Sch Med, Dept Surg, Stanford, CA 94305 USA
3.Fujian Coll Hlth Occupat & Technol, Fuzhou 350001, Peoples R China
4.Baotou Med Coll, Baotou 014060, Peoples R China
5.Peking Univ, Sch Basic Med Sci, Dept Cell Biol, Beijing, Peoples R China
6.Peking Univ, Sch Basic Med Sci, Stem Cell Res Ctr, Beijing, Peoples R China
7.China Med Univ, Shengjing Hosp, Shenyang 110004, Peoples R China
8.Beijing Obstet & Gynecol Hosp, Dept Reprod Med, Beijing 100026, Peoples R China
9.Peking Univ, Med & Hlth Anal Ctr, Beijing 100191, Peoples R China

Recommended Citation:
Li, Yang,Hong, Wan Xing,Lan, Baojin,et al. PDGF mediates derivation of human embryonic germ cells[J]. DIFFERENTIATION,2013,86(4-5):141-148.
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