IR@PKUHSC  > 基础医学院  > 细胞生物学系
学科主题基础医学
PDGF mediates derivation of human embryonic germ cells
Li, Yang1,2,3; Hong, Wan Xing4,5; Lan, Baojin6; Xu, Xiaoyan3; Liu, Yinan1,2; Kong, Lin3; Li, Yaxuan3; Zhou, Shixin1,2; Liu, Ying7; Feng, Ruopeng8; Jiang, Sibo1,2; He, Qihua9; Tan, Jichun3
关键词PDGF Human embryonic germ cells Derivation
刊名DIFFERENTIATION
2013-11-01
DOI10.1016/j.diff.2013.11.002
86期:4-5页:141-148
收录类别SCI
文章类型Article
WOS标题词Science & Technology
类目[WOS]Cell Biology ; Developmental Biology
资助者National Natural Science Foundation of China (NSFC) Projects ; Beijing Natural Science Foundation Project ; Ph.D. Programs Foundation of Ministry of Education of China ; Inner Mongolia Natural Science Foundation ; National Natural Science Foundation of China (NSFC) Projects ; Beijing Natural Science Foundation Project ; Ph.D. Programs Foundation of Ministry of Education of China ; Inner Mongolia Natural Science Foundation
研究领域[WOS]Cell Biology ; Developmental Biology
关键词[WOS]PLURIPOTENT STEM-CELLS ; GROWTH-FACTOR ; IN-VITRO ; CULTURE ; SPHINGOSINE-1-PHOSPHATE ; BLASTOCYSTS ; EXPRESSION ; PROMOTES ; MARKERS ; ROLES
英文摘要

Human embryonic germ cells (hEGCs) are a valuable and underutilized source of pluripotent stem cells. Unlike embryonic stem cells, which have been extensively studied, little is known about the factors that regulate hEGC derivation and maintenance. This study demonstrates for the first time a central role for selective activation of PDGFR signaling in the derivation and maintenance of pluripotency in hEGCs. In the study, hEGCs were found to express PDGF receptor a at high levels compared to human embryonic stem cells (hESCs). PDGF significantly improved formation of alkaline phosphatase (AP) positive hEGC colonies. We subsequently determined that PDGF activates the phosphatidylinositol-3-kinase (P13K) pathway as phosphorylation of AKT was up-regulated in response to PDGF. Furthermore, inhibition of PI3K signaling using small molecular inhibitor LY294002 led to significantly decreased AP positive hEGC colony formation whereas inhibition of MAPK pathway using U0126 had a negligible effect. We established a primary mechanism for PDGF mediated derivation and maintenance of hEGCs by demonstrating that OCT4 was upregulated and PTEN was suppressed in a dose dependent manner in response to PDGF. (C) 2014 International Society of Differentiation. Published by Elsevier BM. All rights reserved.

语种英语
所属项目编号31000653 ; 5112019 ; 20100001120044 ; 2012MS1142
资助者National Natural Science Foundation of China (NSFC) Projects ; Beijing Natural Science Foundation Project ; Ph.D. Programs Foundation of Ministry of Education of China ; Inner Mongolia Natural Science Foundation ; National Natural Science Foundation of China (NSFC) Projects ; Beijing Natural Science Foundation Project ; Ph.D. Programs Foundation of Ministry of Education of China ; Inner Mongolia Natural Science Foundation
WOS记录号WOS:000335297100001
引用统计
被引频次:1[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.bjmu.edu.cn/handle/400002259/61818
专题基础医学院_细胞生物学系
作者单位1.Stanford Sch Med, Dept Radiol, Stanford, CA 94305 USA
2.Stanford Sch Med, Dept Surg, Stanford, CA 94305 USA
3.Fujian Coll Hlth Occupat & Technol, Fuzhou 350001, Peoples R China
4.Baotou Med Coll, Baotou 014060, Peoples R China
5.Peking Univ, Sch Basic Med Sci, Dept Cell Biol, Beijing, Peoples R China
6.Peking Univ, Sch Basic Med Sci, Stem Cell Res Ctr, Beijing, Peoples R China
7.China Med Univ, Shengjing Hosp, Shenyang 110004, Peoples R China
8.Beijing Obstet & Gynecol Hosp, Dept Reprod Med, Beijing 100026, Peoples R China
9.Peking Univ, Med & Hlth Anal Ctr, Beijing 100191, Peoples R China
推荐引用方式
GB/T 7714
Li, Yang,Hong, Wan Xing,Lan, Baojin,et al. PDGF mediates derivation of human embryonic germ cells[J]. DIFFERENTIATION,2013,86(4-5):141-148.
APA Li, Yang.,Hong, Wan Xing.,Lan, Baojin.,Xu, Xiaoyan.,Liu, Yinan.,...&Tan, Jichun.(2013).PDGF mediates derivation of human embryonic germ cells.DIFFERENTIATION,86(4-5),141-148.
MLA Li, Yang,et al."PDGF mediates derivation of human embryonic germ cells".DIFFERENTIATION 86.4-5(2013):141-148.
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